Computational and experimental model of electroporation for human aorta

• Autorzy: Filipovic N. , Saveljic I. , Jovicic N. , Tanaskovic I. , Zdravkovic N.

Identyfikatory

DOI

10.5277/ABB-00444-2015-02

• Języki publikacji: EN

Abstrakty

EN

Purpose: In this study the computational and experimental electroporation model with human aorta tissue is made in order to examine the reduction of smooth muscle cells. Methods. The segments in native state of the aorta are treated by electroporation method through a series of electrical impulses from 50 V/cm to 2500 V/cm. For each patient we analyzed one sample with and one sample without electroporation as a control. In the computational study, electrical field distribution is solved by the Laplace equation. The Pennes Bioheat equation without metabolism and blood perfusion heating is used to solve heat transfer problems. Different conductivity values are used in order to fit the experimental results. Results: Experimental histology has shown us that there are a smaller number of vascular smooth muscle cells (VSMC) nuclei at the tunica media, while the elastic fibre morphology is maintained 24 h after electroporation. In the computational model, heat generation coupled with electrical field is included. The fitting procedure is applied for conductivity values in order to make material properties of the aorta tissue. The fitting procedure gives tissue conductivity of 0.44 [S/m] for applied electrical field of 2500 V/cm. Conclusions: Future studies are necessary for investigation of a new device for in-vivo ablation with electroporation of plaque stenosis. It will open up a new avenue for stenosis treatment without stent implantation.

Słowa kluczowe

EN

electroporation; heat generation; ablation; VSMC reduction; fitting; conductivity

PL

elektroporacja; wytwarzanie ciepła; ablacja; kształtka wodociągowa; przewodnictwo cieplne

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2016
• Tom: Vol. 18, no. 4
• Strony: 15--20
• Opis fizyczny: Bibliogr. 14 poz., rys., wykr.

Twórcy

autor

Filipovic N.

• Faculty of Engineering, University of Kragujevac, 34000 Kragujevac, Serbia

autor

Saveljic I.

• Faculty of Engineering, University of Kragujevac, 34000 Kragujevac, Serbia

autor

Jovicic N.

• Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia

autor

Tanaskovic I.

• Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia

autor

Zdravkovic N.

• Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia

Bibliografia

• [1] BANCROFT J.D., GAMBLE M., Theory and practice of histological techniques, 5th ed., Churchill Livingstone, Edinburgh, London, New York, Oxford, 2002.
• [2] DAVALOS R., MIR L., RUBINSKY B., Tissue ablation with irreversible electroporation, Annals Biomed. Eng., 2005, 33, 223–231.
• [3] DENG Z.S., LIU J., Blood Perfusion-Based Model for Characterizing the Temperature Fluctuations in Living Tissue, Phys. A STAT Mech. Appl., 2001, 300, 521–530.
• [4] DUCK F.A., Physical Properties of Tissues: A Comprehensive Reference Book, San Diego, Academic Press, 1990.
• [5] EDD J.F., DAVALOS R.V., Mathematical Modeling of Irreversible Electroporation for Treatment Planning, Technology in Cancer Research and Treatment, ISSN 1533-0346, August 2007, 6(4).
• [6] HAMILTON W.A., SALE J.H., Effects of high electrical fields on microorganisms. II. Mechansims of action of the lethal effect, Biochimica et Biophysica Acta, 1967, 148, 789–800.
• [7] LACKOVIC I., MAGJAREVIC R., MIKLAVCIC D., Threedimensional Finite-element Analysis of Joule Heating in Electrochemotherapy and in vivo Gene Electrotransfer, IEEE Transactions on Dielectrics and Electrical Insulation, 2009, 16(5), 1338–1347.
• [8] MAOR E., IVORRA A., LEOR J., RUBINSKY B., The Effect of Irreversible Electroporation on Blood Vessels, Technology in Cancer Research and Treatment, ISSN 1533-0346, August 2007, 6(4), 307–312.
• [9] NELDER J., MEAD R., A simplex method for function minimization, Computer Journal, 1965, 7, 308–313.
• [10] SALE A.J.H., HAMILTON W.A., Effects of high electri fields on microorganisms III. Lysis of erythrocytes and protoplasts, Biochimica et Biophysica Acta, 1968, 163, 37–43.
• [11] SALE J.H., HAMILTON W.A., Effects of high electrical fields on microorganisms. I. Killing of bacteria and yeast, Biochimica and Biophysisca Acta, 1967, 148, 781–788.
• [12] SANO M.B., NEAL R.E., GARCIA P.A., GERBER D., ROBERTSON J., DAVALOS RV., Towards the creation of decellularized organ constructs using irreversible electroporation and active mechanical perfusion, BioMedical Engineering OnLine, 2010, 9: http://www.biomedical-engineeringonline.com/content/9/1/83 (2010).
• [13] SEL D., CUKJATI D., BATIUSKAITE D., SLIVNIK T., MIR L.M., MIKLAVCIC D., Sequential Finite Element Model of Tissue Electropermeabilization, IEEE Trans. Biomed. Eng., 2005, 52, 816–827.
• [14] STARY H.C., CHANDLER A.B., DINSMORE R.E., FUSTER V., GLAGOV S., INSULL W.Jr., ROSENFELD M.E., SCHWARTZ C.J., WAGNER W.D., WISSLER R.W., A definition of advanced types of atherosclerotic lesions and a histological classification of atherosclerosis, American Heart Association, A report from the Committee on Vascular Lesions of the Council on Arteriosclerosis, Circulation, 1995, 92, 1355–1374.