HPLC determination of enantiomeric thiazolidine-2-carboxylic acid on chiral stationary phase with pre-column derivatization

• Autorzy: Zhang Y. , Peng Q. , Zeng H. , Yao S , Zhang Y. , Song H.
• Języki publikacji: EN

Abstrakty

EN

A new high-performance liquid chromatography (HPLC) method has been developed and validated for determination of enantiomeric purity of thiazolidine-2-carboxylic acid within a short run time of less than 10 min. The method was based on pre-column derivatization of thiazolidine-2-carboxylic acid with aniline, and complete separation of enantiomers has been achieved on a Chiralcel OD-H analytical column (250 × 4.6 mm) using n-hexane-isopropanol (85:15 v/v) as mobile phase at a flow rate of 1.0 mL min^-1 under UV and optical rotation (OR) detection. Detection wavelength was set at 254 nm. Then the effects of mobile phase and temperature on enantioselectivity were further evaluated. The method was validated with respect to precision, accuracy, linearity, limit of detection (LOD), limit of quantification (LOQ), and robustness. The recoveries were between 98.5 and 101.3% with percentage relative standard deviation less than 1.16%. The LOD and LOQ for the aniline derivatives of (+)-thiazolidine-2-carboxylic acid were 4.9 and 16.4 μg mL^-1 and for the aniline derivatives (−)-thiazolidine-2-carboxylic acid were 5.1 and 17.2 μg mL^-1, respectively.

Słowa kluczowe

EN

liquid chromatography; enantiomeric purity; thiazolidine-2-carboxylic acid; pre-column derivatization; method validation

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2013
• Tom: Vol. 25, no. 2
• Strony: 287--296
• Opis fizyczny: Bibliogr. 23 poz., rys., tab.

Twórcy

autor

Zhang Y.

• Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China

autor

Peng Q.

• Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China

autor

Zeng H.

• Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China

autor

Yao S

• Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China

autor

Zhang Y.

• Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China

autor

Song H.

• Sichuan University Department of Pharmaceutical and Biological Engineering Chengdu 610065 China

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