Concept and realization of backpack-type system for multichannel electrophysiology in freely behaving rodents

• Autorzy: Chaikovska Olga , Ponomarenko Olexandr , Dovgan Olexandr , Rokunets Igor , Pavlov Sergii , Kryvoviaz Olena , Vlasenko Oleg

Identyfikatory

DOI

10.35784/IAPGOS.688

Warianty tytułu

PL

Koncepcja i realizacja systemu plecakowego dla wielokanałowej elektrofizjologii u swobodnie zachowujących się gryzoni

• Języki publikacji: EN

Abstrakty

EN

Technologies for multichannel electrophysiology are experiencing astounding growth. Numbers of channels reach thousands of recording sites, systems are often combined with electrostimulations and optic stimulations. However, the task of design the cheap, flexible system for freely behaving animals without tethered cable are not solved completely. We propose the system for multichannel electrophysiology for both rats and mice. The system allows to record unit activity and local field potential (LFP) up to 32 channels with different types of electrodes. The system was constructed using Intan technologies RHD 2132 chip. Data acquisition and recordings take place on the DAQ-card, which is placed as a back-pack on the animal. The signal is amplified with amplifier cascade and digitalized with 16-bit ADC. Instrumental filters allow to filter the signal in 0.1–20000 Hz bandwidth. The system is powered from the mini-battery with capacity 340 mA/hr. The system was validated with generated signals, in anaesthetized rat and showed a high quality of recordings.

PL

Technologie elektrofizjologii wielokanałowej odnotowują zdumiewający wzrost. Liczba kanałów dociera do tysięcy miejsc rejestracji, systemy często łączone są z elektrostymulacjami i stymulacjami optycznymi. Jednak zadanie zaprojektowania taniego, elastycznego systemu pozwalającego na swobodne zachowania zwierząt bez przywiązanego kabla nie zostało całkowicie rozwiązane. Zaproponowano system wielokanałowej elektrofizjologii zarówno dla szczurów, jak i myszy. System pozwala rejestrować aktywność jednostki i potencjał pola lokalnego (LFP) do 32 kanałów z różnymi rodzajami elektrod. System został zbudowany przy użyciu technologii Intan RHD 2132. Akwizycja danych i nagrania odbywają się na karcie DAQ, która została umieszczona w plecaku zwierzęcia. Sygnał jest wzmacniany kaskadą wzmacniaczy i digitalizowany za pomocą 16-bitowego przetwornika ADC. Filtry pozwalają filtrować sygnał w paśmie 0,1–20000 Hz. Zasilany jest z mini-baterii o wydajności 340 mA/godz. System został zwalidowany generowanymi sygnałami u znieczulonego szczura i wykazał wysoką jakość nagrań.

Słowa kluczowe

EN

multichannel electrophysiology; freely behaving rodent; unit activity; local field potential

PL

elektrofizjologia wielokanałowa; gryzoń swobodnie zachowujący się; aktywność jednostkowa; potencjał pola lokalnego

• Wydawca: Wydawnictwo Politechniki Lubelskiej
• Czasopismo: Informatyka, Automatyka, Pomiary w Gospodarce i Ochronie Środowiska
• Rocznik: 2019
• Tom: T. 9, nr 4
• Strony: 64--68
• Opis fizyczny: Bibliogr. 29 poz., rys., tab.

Twórcy

autor

Chaikovska Olga

• National Pirogov Memorial Medical University, Vinnytsia, Ukraine

autor

Ponomarenko Olexandr

• National Pirogov Memorial Medical University, Vinnytsia, Ukraine

autor

Dovgan Olexandr

• National Pirogov Memorial Medical University, Vinnytsia, Ukraine

autor

Rokunets Igor

• National Pirogov Memorial Medical University, Vinnytsia, Ukraine

autor

Pavlov Sergii

• Vinnytsia National Technical University, Vinnytsia, Ukraine

autor

Kryvoviaz Olena

• National Pirogov Memorial Medical University, Vinnytsia, Ukraine

autor

Vlasenko Oleg

• National Pirogov Memorial Medical University, Vinnytsia, Ukraine

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).