Investigation of the toxicity of the ionic liquid 1-butyl-3-methylimidazolium chloride to Saccharomyces cerevisiae AY93161 for lignocellulosic ethanol production

• Autorzy: Zhu Shengdong , Yu Pei , Lei Mingke , Tong Yanjie , Zheng Lu , Zhang Rui , Ji Jun , Chen Qiming , Wu Yuanxin
• Języki publikacji: EN

Abstrakty

EN

Ionic liquid (IL) pretreatment of lignocellulosic materials has provided a new technical tool to improve lignocellulosic ethanol production. To evaluate the influence of the residual IL in the fermentable sugars from enzymatic hydrolysis of IL pretreatment of lignocellulosic materials on the subsequent ethanol fermentation, the toxicity of the IL 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) to Saccharomyces cerevisiae AY93161 was investigated. Firstly, the morphological structure, budding and metabolic activity of Saccharomyces cerevisiae AY93161 at different [BMIM]Cl concentrations were observed under an optical microscope. The results show that its single cell morphology remained unchanged at all [BMIM]Cl concentrations, but its reproduction rate by budding and its metabolic activity decreased with the [BMIM]Cl concentration increasing. The half effective concentration (EC50) and the half inhibition concentration (IC50) of [BMIM]Cl to Saccharomyces cerevisiae AY93161 were then measured using solid and liquid suspension culture and their value were 0.53 and 0.39 g.L-1 respectively. Finally, the influence of [BMIM]Cl on ethanol production was investigated. The results indicate that the [BMIM]Cl inhibited the growth and ethanol production of Saccharomyces cerevisiae AY93161. This toxicity study provides useful basic data for further development in lignocellulosic ethanol production by using IL technology and it also enriches the IL toxicity data.

Słowa kluczowe

EN

Ionic liquid; [bmim]Cl; toxicity; Saccharomyces cerevisiae AY93161; lignocellulosic ethanol production

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2013
• Tom: Vol. 15, nr 2
• Strony: 94–98
• Opis fizyczny: Bibliogr. 18 poz., rys., tab., wykr.

Twórcy

autor

Zhu Shengdong

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

autor

Yu Pei

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

autor

Lei Mingke

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

autor

Tong Yanjie

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

autor

Zheng Lu

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

autor

Zhang Rui

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

autor

Ji Jun

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

autor

Chen Qiming

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

autor

Wu Yuanxin

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

Bibliografia

• 1. Kokorin, A. (2011). Ionic Liquids: Applications and Perspectives. InTech. DOI: 10.5772/1782.
• 2. Wasserscheid, P. & Stark, A. (2010). Handbook of Green Chemistry: Green Solvents, Vol. 6-Ionic Liquid. Wiley-VCH, Germany.
• 3. Zhu, S. (2008). Use of ionic liquids for the efficient utilization of lignocel-lulosic materials. J. Chem. Technol. Biotechnol. 83, 777-779, DOI: 10.1002/jctb.1884.
• 4. Wang, Q., Wu, Y. & Zhu, S. (2011). Use of ionic liquids for improvement of cellulosic ethanol production. Bioresources. 6 (1), 1-2.
• 5. Mora-Pale, M., Meli, L., Doherty, T.V., Linhardt, R.J. & Dordick, J.S. (2011). Room temperature ionic liquids as emerging solvents for the pretreatment of lignocellulosic biomass. Biotechnol. Bioeng. 108 (6): 1229-1245, DOI: 10.1002/bit.23108.
• 6. Simmons, B.A., Singh, S., Holmes, B.M. & Blanch, H.W. (2010). Ionic liquid pretreatment. Chem. Eng. Prog., 116 (3), 50-55.
• 7. Tadesse, H. & Luque, R. (2011). Advances on biomass pretreatment using ionic liquid: an overview. Energy Environ. Sci., 4, 3913-3929, DOI: 10.1039/C0EE00667J.
• 8. Zhu, S., Yu, P., Tong, Y., Chen, R., Lv, Y., Zhang, R., Lei, M., Ji, J., Chen, Q. & Wu, Y. (2012). Effects of the ionic liquid 1-butyl-3-methylimidazolium chloride on the growth and ethanol fermentation of Saccharomyces cerevisiae AY92022. Chem. Biochem. Eng. Q., 26, 105-109.
• 9. Pham, T.P.T., Cho, C.W. & Yun, Y.S. (2010). Environmental fate and toxicity of ionic liquids: a review. Water Res., 44, 352-372, DOI: 10.1016/j.watres.2009.09.030.
• 10. Zhu, S., Wu, Y., Chen, Q., Chi, R., Shen, X. & Yu, Z. (2009). A mini-review on greenness of ionic liquids. Chem. Biochem. Eng. Q., 23, 207-211.
• 11. Zhao, D., Liao, Y. & Zhang, Z. (2007). Toxicity of ionic liquids. Clean-Soil, Air, Water, 35, 42-48, DOI: 10.1002/ clen.200600015.
• 12. Docherty, K.M. & Kulpa, C.F. (2005). Toxicity and antimicrobial activity of imidazolium and pyridinium ionic liquids. Green Chem., 7, 185-189, DOI: 10.1039/B419172B.
• 13. Painting, K. & Kirsop, B. (1990). A quick method for estimating the percentage of viable cells in a yeast population, using methylene blue staining. World J. Microbiol. Biotechnol., 6 (3), 346-347, DOI: 10.1007/BF01201311.
• 14. Atala, D.I.P., Costa, A.C., Filho, M.R. & Maugeri, F. (2001). Kinetics of ethanol fermentation with high biomass concentration considering the effect of temperature. Appl. Biochem. Biotechnol., 91-93, 353-365, DOI: 10.1385/ABAB:91-93:1-9:353.
• 15. Zhu, S., Wu, Y., Yu, Z., Zhang, X., Wang, C., Yu, F., Jin, S., Zhao, Y., Tu, S. & Xue, Y. (2005). Simultaneous saccharification and fermentation of microwave/alkali pretreated rice straw to ethanol. Biosys. Eng., 92 (2), 229-235, DOI: 10.1016/j.biosystemseng.2005.06.012.
• 16. Miller, G.L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31, 426-428.
• 17. Lee, S.M., Chang, W.J., Choi, A.R. & Koo, Y.M. (2005). Influence of ionic liquids on the growth of Escherichia coli. Korean J. Chem. Eng., 22, 687-690, DOI: 10.1007/BF02705783.
• 18. Tong, Y., Wang, Q., Bi, Y., Lei, M., Lv, Y., Liu, Y., Liu, J., Lu, L., Ma, Y., Wu, Y. & Zhu, S. (2012). Influence of ionic liquid 1- butyl-3-methylimidazolium chloride on the soil micro-ecological system. Open Biotechnol. J., 6, 1-4.