Genotoxicity of cyclopentha[c]phenanthrene and its two derivatives based on an in vitro micronucleus test

• Autorzy: Rudnicka K. , Łuczyński M. K. , Góra M.
• Języki publikacji: EN

Abstrakty

EN

Environmental pollution causes a variety of health problems, including cancer. Many known pollutants have carcinogenic properties and polycyclic aromatic hydrocarbons (PAH) belong to this group. In this study, an in vitro culture of V79 cells of the Chinese hamster was subjected to three tested PAHs: 5-amino-2,3-dihydro-1H-cyclopenta-[c]phenanthrene (ACP[c]Ph), 5-amino-9-methoxy-2,3-dihydro-1H-cyclopenta[c]phenanthrene (AMCP[c]Ph) and cyclopenta[c]phenanthrene (CP[c]Ph). The in vitro micronucleus (MN) assay was applied in order to evaluate the genotoxic properties of the studied compounds. The highest genotoxic effect was observed for AMCP[c]Ph in a concentration of 0.02μg·ml-1. The genotoxic effect of the other two compounds was slightly lower.

Słowa kluczowe

EN

derivatives; genotoxicity; in vitro micronucleus test; PAHs; V79

PL

pochodne; genotoksyczność; PAHs; test mikrojądrowy in vitro; V79

• Wydawca: University of Warmia and Mazury in Olsztyn, Poland
• Czasopismo: Environmental Biotechnology
• Rocznik: 2012
• Tom: Vol. 8, No. 2
• Strony: 63--66
• Opis fizyczny: Bibliogr. 17 poz., rys.

Twórcy

autor

Rudnicka K.

• Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Słoneczna 45G, 10-718 Olsztyn, Poland

autor

Łuczyński M. K.

• Department of Chemistry, University of Warmia and Masury in Olsztyn, Plac Łódzki 4, 10-957 Olsztyn, Poland

autor

Góra M.

• Department of Organic Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland

Bibliografia

• Ahmad, K., Jaber Saleh. 2010. Clastogenic studies on Tandaha Dam water in Asserian Black Sea. Mediterranean Environment 16: 33-42.
• Coombs, M.M., T.S. Bhatt, C.J. Croft. 1973. Correlation between carcinogenicity and chemical structure in cyclopenta[c]phenanthrenes. Cancer Research 33: 832-837.
• Doehmer, J. 1993. V79 Chinese hamster cells genetically engineered for cytochrome P450 and their use in mutagenicity and metabolism studies. Toxicology 82: 105-118.
• Fenech, M. 1993. The cytokinesis-block micronucleus technique and its application to genotoxicity studies in human populations. Environmental Health Perspectives Supplements 101: 101-107.
• Góra, M., M.K. Łuczyński, J.J. Sepioł. 2005. A convenient synthesis of 2-naphthylcyclopentanones and 2-naphthyl-cyclohexanones from 1-naphthylcycloalkenes. Synthesis 10: 1625-1630.
• Heddle, J.A., M.F Salamone, M. Hite, B. Kirkhart, K. Mavournin, J.G. MacGregor, G.W. Newell. 1983. The induction of micronuclei as a measure of genotoxicity. Mutation Research 123: 61-118.
• Heddle, J.A., M.C. Cimino, M. Hayashi, F. Romagna, M.D. Shelby, J.D. Tucker, P. Vanparys, J.T. MacGregor. 1991. Micronuclei as an index of cytogenetic damage: past, present and future. Environmental Molecular Mutagenesis 18: 277-291.
• Kirsch-Volders, M., G. Plas, A. Elhajouji, M. Lukamowicz, L. Gonzalez, K.V. Loock, I. Decordier. 2011. The in vitro MN assay in 2011: origin and fate, biological significance, protocols, high throughput methodologies and toxicological relevance. Archives of Toxicology 85: 873-899.
• ISO 21427-2: 2006. Water Quality-Evaluation of Genotoxicity by Measurement of the Induction of Micronuclei-Part 2: Mixed Population Method Using the Cell Line V79.
• Łuczyński, M.K., M. Góra, D. Mielżyńska, P. Brzuzan, S. Tejs, P. Pirogowicz, M. Luczynski. 2007. Preliminary evaluation of mutagenic activity of two amino derivatives of cyclopenta[c]phenanthrene. Environmental Biotechnology 3: 15-19.
• Marrocchi, A., L. Minuti, G. Morozzi, L. Pampanella, A. Taticchi. 1996. Synthesis and mutagenicity of some cyclopenta[c]phenanthrenes and indo[c]phenanthrenes. Carcinogenesis 17: 2009-2012.
• Rudnicka, K., Z. Krzyżewski. 2011. Selection and optimization of staining techniques of Chinese hamster lung fibroblasts V79 cell preparations. Environmental Biotechnology 7: 84-87.
• Shaw, G.R., D.W. Conell. 1994. Prediction and monitoring of the carcinogenicity of polycyclic aromatic compounds (PACs). Environmental Contamination Toxicology 135: 1-62.
• Villarini, M., M. Moretti, R. Pasquini, G. Scassellati-Sforzolini, C. Fatigoni, M. Marcarelli, S. Monarca, A.V. Rodriguez. 1998. In vitro genotoxic effects of the insecticide deltamethrin in human peripheral blood leukocytes: DNA damage ('comet' assay) in relation to the induction of sister-chromatid exchanges and micronuclei. Toxicology 130: 129-139.
• Wise, A.S., B.A. Benner, S.N. Chesler, L.R. Hilpert, C.R. Vogt, W.E. May. 1986. Characterization of the polycyclic aromatic hydrocarbons from two standard reference material air particulate samples. Analytical Chemistry 58: 3067-3077.
• Wood, A.W., R.L. Chang, W. Levin, D.E. Ryan, P.E. Thomas, He Duck Mah, J.M. Karie, H. Yagi, D.M. Jerina, A.H. Conney. 1979. Mutagenicity and tumorgenicity of phenanthrene and chrysene epoxides and diol epoxides. Cancer Research 39: 4069-4077.
• Żelazna, K., K. Rudnicka, S. Tejs. 2011. In vitro micronucleus test assessment of polycyclic aromatic hydrocarbons. Environmental Biotechnology 7: 70-80.