Development of validated UHPLC–PDA with ESI–MS-MS method for concurrent estimation of magnoflorine, berbamine, columbamine, jatrorrhizine, palmatine and berberine in Berberis aristata

• Autorzy: Basera Ishita A. , Girme Aboli , Bhatt Vijay P. , Saste Ganesh , Pawar Sandeep , Hingorani Lal , Shah Mamta B.

Identyfikatory

DOI

10.1556/1326.2021.00960

• Języki publikacji: EN

Abstrakty

EN

A validated UHPLC-PDA with an ESI-MS/MS method has been developed for simultaneous estimation of six bioactive alkaloids (magnoflorine, berbamine, columbamine, jatrorrhizine, palmatine and berberine) in the different extracts of the roots of Berberis aristata DC (Family:Berberdiaceae). It is an important medicinal herb native to Northern Himalaya and commonly known as ‘daruharidra’, ‘daruhaldi’, ‘Indian barberry’ or ‘tree turmeric’. An insight into the research literature uncovered reports on isoquinoline alkaloids like magnoflorine, berbamine, columbamine, jatrorrhizine, palmatine, and berberine as major bioactives in B. aristata roots, possessing different pharmacological and therapeutic effects. In the present study, these aforementioned alkaloids were separated on Phenomenex Luna®, 5 µm-C8 analytical column. The HPLC-MS analysis was performed at a flow rate of 0.90 mL min⁻¹. Each alkaloid that is resolved was characterized by precursor ions and fragment ions with electrospray ionization (ESI) source in both positive and negative ionization using scan mode. The limit of detections (LODs) were 0.087, 0.727, 0.035, 0.124, 0.782 and 0.794 μg mL⁻¹ for magnoflorine, berbamine, columbamine, jatrorrhizine, palmatine and berberine, respectively. The proposed UHPLC-PDA method was fully validated according to international (ICH) guidelines and was found to be selective, sensitive and highly accurate for the concomitant estimation of the aforementioned symbolic bio-markers of B. aristata roots.

Słowa kluczowe

EN

berberine; berbamine; columbamine; simultaneous UHPLC-MS/MS; jatrorrhizine; magnoflorine; palmatine

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2022
• Tom: Vol. 34, no. 4
• Strony: 412--421
• Opis fizyczny: Bibliogr. 32 poz., tab., rys., wykr.

Twórcy

autor

Basera Ishita A.

• Department of Pharmacognosy, L. M. College of Pharmacy, Gujarat Technological University, Ahmedabad, Gujarat, India

• https://orcid.org/0000-0001-6199-2267

autor

Girme Aboli

• Pharmanza Herbal Pvt. Ltd., Anand, 388435, Gujarat, India

• https://orcid.org/0000-0002-1536-1656

autor

Bhatt Vijay P.

• Herbal Research & Development Institute, Mandal, Gopeshwar, Chamoli, Uttrarakhand, India

autor

Saste Ganesh

• Pharmanza Herbal Pvt. Ltd., Anand, 388435, Gujarat, India

• https://orcid.org/0000-0003-2310-4956

autor

Pawar Sandeep

• Pharmanza Herbal Pvt. Ltd., Anand, 388435, Gujarat, India

• https://orcid.org/0000-0001-6524-7045

autor

Hingorani Lal

• Pharmanza Herbal Pvt. Ltd., Anand, 388435, Gujarat, India

• https://orcid.org/0000-0003-0371-0633

autor

Shah Mamta B.

• Department of Pharmacognosy, L. M. College of Pharmacy, Gujarat Technological University, Ahmedabad, Gujarat, India

• https://orcid.org/0000-0002-8611-2315

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).