Phytochelatins of Cladophora rupestris in Pb2+ absorption and its detoxification

• Autorzy: Zhang Lu-Sheng , Yang Liu , Li Lingsheng , Feng Xiaoyu , Cao De-Ju , Liu Zhao-Wen

Identyfikatory

DOI

10.37190/epe230303

• Języki publikacji: EN

Abstrakty

EN

Phytochelatins (PCs) exist widely in plants and are closely related to plant resistance to heavy metals (HMs). Glutathione (GSH) and nonprotein thiols (NPTs) are the major components of PCs. This study investigates the role of the PCs of Cladophora rupestris in Pb²⁺ accumulation and detoxification. The distribution of Pb²⁺ in the PCs of C. rupestris was studied. FTIR and XPS are used to characterize the chelating power of Pb²⁺ with PCs in C.rupestris. The curve fitting of the secondary protein structure is used to identify the functional groups with Pb²⁺. Results showed that the content of Pb²⁺ in the PCs of C. rupestris increased with an increase in Pb²⁺ stress. Pb content increased to 352 and 314 mg/kg in NPTs and GSH, respectively, when Pb stress concentration reached 7.5 mg/dm³ . The Pb²⁺ fraction of C. rupestris PCs reached a maximum of 10.8 and 9.3% in NPTs and GSH, respectively. The Pb²⁺ uptake by GSH and NPTs was 40–48% and 52–60%, respectively. Pb2+ bound with the PCs of C. rupestris, forming complexes that contained Pb–OOC, Pb–C=O, CO–Pb, –N=Pb, Pb–NH2, Pb–O, Pb–N–, Pb–C–, Pb–S, and Pb2+ with multiple groups of PCs as bridging ligand atoms.

Słowa kluczowe

EN

phytochelatins; heavy metals; detoxification; Cladophora rupestris; Pb+

PL

fitochelatyny; metale ciężkie; detoksykacja; Cladophora rupestris; Pb+

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Environment Protection Engineering
• Rocznik: 2023
• Tom: Vol. 49, nr 3
• Strony: 31--42
• Opis fizyczny: Bibliogr. 25 poz., rys., tab.

Twórcy

autor

Zhang Lu-Sheng

• Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China

autor

Yang Liu

• Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China

autor

Li Lingsheng

• Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China

autor

Feng Xiaoyu

• Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China

autor

Cao De-Ju

• Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China

autor

Liu Zhao-Wen

• Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
• School of Materials and Environmental Engineering, Chizhou University, Chizhou 247000, China

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