The influence of selected ionic liquids on activity of chitin deacetylase

• Autorzy: Aspras I. , Kamińska M. , Karzyński K. , Kawka M. , Jaworska M. M.

Identyfikatory

DOI

10.1515/cpe-2016-0008

• Języki publikacji: EN

Abstrakty

EN

Chitin deacetylase is the only known enzyme which is able to deacetylate N-acetyl-D-glucosamine units in chitin or chitosan chains. As chitin can hardly be dissolved in organic/inorganic solvents, new solvents are still searched. Ionic liquids are promising for that application and for homophase enzymatic deacetylation. The aim of the work was to investigate the influence of selected ionic liquids on activity of chitin deacetylase. It has been shown that [Amim] ionic liquids increase the activity of chitin deacetylase. The highest activity was observed for [Amim][Cl]. Ionic liquids with shorter (ethyl (C2)) and longer side chain (buthyl (C4)) only insignificantly influenced the activity of the enzyme. All tested ionic liquids with [Br] anion increased the activity of chitin deacetylase while the [Emim] and [Bmim] cation in combination with [Cl] anion inhibited the activity of the enzyme.

Słowa kluczowe

EN

chitin deacetylase; enzyme activity; imidasolium ionic liquids; pyridinium ionic liquids

PL

aktywność enzymu; ciecze jonowe

• Wydawca: Komitet Inżynierii Chemicznej i Procesowej Polskiej Akademii Nauk
• Czasopismo: Chemical and Process Engineering
• Rocznik: 2016
• Tom: Vol. 37, nr 1
• Strony: 77--82
• Opis fizyczny: Bibliogr. 6 poz., wykr.

Twórcy

autor

Aspras I.

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, Poland

autor

Kamińska M.

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, Poland

autor

Karzyński K.

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, Poland

autor

Kawka M.

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, Poland

autor

Jaworska M. M.

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, Poland

Bibliografia

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• 2. Gorke J., Srienc F., Kazlauskas R., 2010. Toward advanced ionic liquids. Polar, enzyme-friendly solvents for biocatalysis. Biotech. Bioproc. Eng., 15, 40-53. DOI: 10.1007/s12257-009-3079-z.
• 3. Jaworska M.M., Konieczna E., 2001. The influence of supplemental components in nutrient medium on chitosan formation by the fungus Absidia orchidis. Appl. Microbiol. Biotechnol., 56, 220-224. DOI: 10.1007/s002530000591.
• 4. Jaworska M.M., 2012. Kinetics of enzymatic deacetylation of chitosan. Cellulose, 19, 363-369. DOI: 10.1007/s10570-012-9650-3.
• 5. Jaworska M.M., Koźlecki T., Górak A., 2012. Review of the application of ionic liquids as solvents for chitin. J. Polym. Eng., 32, 67-69. DOI: 10.1515/polyeng-2011-0145.
• 6. Naushada M., Al Othman Z.A., Khan A.B., Maroof A., 2012. Effect of ionic liquid on activity, stability, and structure of enzymes: A review. Int. J Biol. Macromol., 51, 555–560. DOI: 10.1016/j.ijbiomac.2012.06.020.