16S rRNA Gen Analysis of Plastic Destruction Bacteries, South Sumatra, Indonesia

• Autorzy: Meiyerani Jeni , Melki Melki , Aryawati Riris , Rozirwan Rozirwan , Ningsih Ellis Nurjuliasti , Wulandari Tuah Nanda Merlia , Nugroho Redho Yoga

Identyfikatory

DOI

10.12911/22998993/178272

• Języki publikacji: EN

Abstrakty

EN

Rivers are the main route for plastic entering the ocean, including the Musi River Estuary. Characteristics of bacteria that are able to degrade plastic waste through polymerase enzymes. The aim of this research is to determine the ability of bacterial isolates to degrade plastic and identify the types of bacteria that degrade plastic waste. This study used plastic bottles, nylon nets, and snack wrappers as objects for degradation measurement. Identification analysis of the 16S rRNA gene using universal PCR primers for bacteria in the form of forward primer 63f (5’-CAG GCC TAA CAC ATG CAA GTC-3’) and reverse primer 1387r (5’-GGG CGG WGT GTA CAA GGC-3’). The type of bacteria with the highest percentage of degradation over 20 days, amounting to 7.75%, was Bacillus amyloliquefaciens. Identification of types of plastic degrading bacteria using 16S rRNA gene analysis showed 11 bacteria with 8 types including Staphylococcus hominis, Pseudomonas aeruginosa, Acinetobacter sp., Acinetobacter baumannii, Acinetobacter variabilis, Shewanella sp., Micrococcus luteus, and Bacillus amyloliquefaciens. The percentage of plastic degradation by bacteria is relatively small, so it is best to look for times where there is potential for bacterial growth.

Słowa kluczowe

EN

16S rRNA; gene analysis; bacteria; degradation; plastic

• Wydawca: Polskie Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology
• Czasopismo: Journal of Ecological Engineering
• Rocznik: 2024
• Tom: Vol. 25, nr 3
• Strony: 85--95
• Opis fizyczny: Bibliogr. 55 poz., rys., tab.

Twórcy

autor

Meiyerani Jeni

• Environmental Management Study Program, Graduate Program, Universitas Sriwijaya, Palembang 30139, South Sumatra, Indonesia

autor

Melki Melki

• Department of Marine Science, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya, Indralaya 30862, South Sumatra, Indonesia

autor

Aryawati Riris

• Department of Marine Science, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya, Indralaya 30862, South Sumatra, Indonesia

autor

Rozirwan Rozirwan

• Department of Marine Science, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya, Indralaya 30862, South Sumatra, Indonesia

• https://orcid.org/0000-0001-8415-3343

autor

Ningsih Ellis Nurjuliasti

• Department of Marine Science, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya, Indralaya 30862, South Sumatra, Indonesia

autor

Wulandari Tuah Nanda Merlia

• National Research and Innovation Agency, Earth and Maritime Research Organization, Research Center for Marine Resources and Inland Aquatic Resources Conservation, Palembang 30137, South Sumatra, Indonesia

autor

Nugroho Redho Yoga

• Department of Marine Science, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya, Indralaya 30862, South Sumatra, Indonesia

• https://orcid.org/0000-0002-7945-4982

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