Simultaneous determination of eleven alkaloids in Corydalis decumbens by HPLC with diode-array detection

• Autorzy: Gu G. H. , Yang D. J. , Wang S. Y. , Zeng W. , Wang W. , Yuan J. S.

Identyfikatory

DOI

10.1556/1326.2017.29.1.11

• Języki publikacji: EN

Abstrakty

EN

A high-performance liquid chromatography—diode-array detection method was developed and validated to determine simultaneously eleven major alkaloids in Corydalis decumbens (Thunb.) Pers. The alkaloids detected were corlumidine, protopine, coptisine, tetrahydrojatrorrhizine, palmatine, berberine, sanguinarine, papaverine hydrochloride, tetrahydropalmatine, bicuculline, and corydaline. Chromatographic separation was achieved using a C-18 column with a mobile phase composed of A (0.2% acetic acid solution, adjusted with triethylamine to pH 5.0) and B (acetonitrile), with stepwise gradient elution. Ultraviolet diode-array detection was used; chromatograms were examined at the wavelength of 280 nm. The regression equations showed a good linear relationship between the peak area of each marker and concentration (r = 0.9994–0.9999). The recovery values ranged between 93.66% and 100.54%. The method was fully validated with respect to detection and quantification limits, precision, reproducibility, and accuracy. The described high-performance liquid chromatography (HPLC) method was successfully used for the differentiation and quantification of the eleven major alkaloids in C. decumbens (Thunb.) Pers. and can be considered an effective procedure for the analyses of this important class of natural compounds.

Słowa kluczowe

EN

Corydalis decumbens; alkaloids; HPLC-DAD; quality control

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2017
• Tom: Vol. 29, no. 1
• Strony: 121--134
• Opis fizyczny: Bibliogr. 34 poz., rys.

Twórcy

autor

Gu G. H.

• Peking University Shenzhen Hospital, Shenzhen, 518036, China

autor

Yang D. J.

• State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen, 518057, China

autor

Wang S. Y.

• State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen, 518057, China

autor

Zeng W.

• Peking University Shenzhen Hospital, Shenzhen, 518036, China

autor

Wang W.

• Hunan University of Chinese Medicine, Changsha, 410208, China

autor

Yuan J. S.

• Peking University Shenzhen Hospital, Shenzhen, 518036, China

Bibliografia

• [1] Committee of National Pharmacopoeia, Pharmacopoeia of P.R. China, Part 1, Chemical Industry Press, Beijing, 2010, pp. 262
• [2] F.N. Ko, T.S. Wu, S.T. Lu, Y.C. Wu, T.F. Huang, and C.M. Teng, Thromb. Res., 56, 289 (1989)
• [3] H. Shiomoto, H. Matsuda, and M. Kubo, Chem. Pharm. Bull., 38, 2320 (1990)
• [4] X.Y. Hu, A.S. Sun, L.M. Yu, and Q. Wu, J. Chin. Integr. Med., 3, 46 (2005)
• [5] L.H. Xu, Z.L. Gu, X.G. Jiang, R. Sheng, X.Q. Wang, and M.L. Xie, Acta Pharm. Sin., 37, 902 (2002)
• [6] K.H. Janbaz, S.A. Saeed, and A.H. Gilani, Pharmacol. Res., 38, 215 (1998)
• [7] H.L. Wei and G.T. Liu, Acta Pharm. Sin., 32, 331 (1997)
• [8] J. Liao, W.Z. Liang, and G.S. Tu, J. Chin. Pharm. Sci., 4, 57 (1995)
• [9] R. Chen, S.H. Yang, and X.L. Tang, Chin. Tradit. Herb. Drugs, 31, 948 (2000)
• [10] D.S. Bae, Y.H. Kim, C.H. Pan, C.W. Nho, J. Samdan, J. Yansan, et al., BMB Rep., 45, 108 (2012)
• [11] C.K. Lai and A.Y. Chan, Clin. Chem., 45, 229 (1999)
• [12] C. Wang, S. Wang, G. Fan, and H. Zou, Anal. Bioanal. Chem., 396, 1731 (2010)
• [13] F.Y. Tang, A.G. Lie, and Y.L. Li, J. Clin. Exp. Med., 5, 185 (2006)
• [14] M. Sun, W. Lou, J.Y. Chun, D.S. Cho, N. Nadiminty, C.P. Evans, et al., Genes Cancer, 1, 283 (2010)
• [15] H. Tsukamoto, S. Kondo, Y. Mukudai, T. Nagumo, A. Yasuda, Y. Kurihara, et al., Anticancer Res., 31, 2841 (2011)
• [16] M. Druzin, D. Haage, and S. Johansson, Neuropharmacology, 46, 285 (2004)
• [17] X.Y. Cheng, Y. Shi, S.L. Zhen, H. Sun, and W. Jin, J. Chromatogr. Sci., 48, 441 (2010)
• [18] J. Ou, L. Kong, C. Pan, X. Su, X. Lei, and H. Zou, J. Chromatogr. A, 1117, 163 (2006)
• [19] C. Wang, Z. Guo, J. Zhang, J. Zeng, X. Zhang, and X. Liang, J. Sep. Sci., 34, 53 (2011)
• [20] J. Zhang, Y. Jin, Y. Liu, Y. Xiao, J. Feng, X. Xue, et al., J. Sep. Sci., 32, 1401 (2009)
• [21] B. Ding, T. Zhou, G. Fan, Z. Hong, and Y. Wu, J. Pharm. Biomed. Anal., 45, 219 (2007)
• [22] H. Ma, Y. Wang, T. Guo, Z. He, X. Chang, and X. Pu, J. Pharm. Biomed. Anal., 49, 440 (2009)
• [23] J. Zhang, Y. Jin, J. Dong, Y. Xiao, J. Feng, X. Xue, et al., Talanta, 78, 513 (2009)
• [24] J. Koyama, I. Morita, A. Kino, K. Iwasa, and K. Tagahara, Chem. Pharm. Bull., 48, 1790 (2000)
• [25] S. Sturm, C. Seger, and H. Stuppner, J. Chromatogr. A, 1159, 42 (2007)
• [26] Q. Yu, S. Tong, J. Yan, C. Hong, W. Zhai, and Y. Li, J. Sep. Sci., 34, 278 (2011)
• [27] S. Kadota, X.L. Sun, P. Basnet, Y. Namba, and Y. Momose, Phytother. Res., 10, 18 (1996)
• [28] Y. Shen, C. Han, Y. Jiang, X. Zhou, Z. Zhu, and X. Lei, Talanta, 84, 1026 (2011)
• [29] X. Wang, Y. Geng, F. Li, X. Shi, and J. Liu, J. Chromatogr. A, 1115, 267 (2006)
• [30] D.J. Wang, J.H. Liu, Y.L. Geng, X. Wang, W.J. Duan, and M.R. Fu, Chin. J. Anal. Chem., 38, 783 (2010)
• [31] S.P. Li, P. Li, C.M. Lai, Y.X. Gong, K.K. Kan, T.T. Dong, et al., J. Chromatogr. A, 1036, 239 (2004)
• [32] R.A. Shaalan and T.S. Belal, J. Chromatogr. Sci., 48, 647 (2010)
• [33] H. Ma, Q. Zhao, Y. Wang, T. Guo, Y. An, and G. Shi, Drug Dev. Ind. Phar., 38, 1200 (2012)
• [34] Z.G. Mei, C.G. Cheng, and J.F. Zheng, J. Tradit. Chin. Med., 31, 311 (2011)

Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).