Immunosensors for human cardiac troponins and CRP, in particular amperometric cTnI immunosensor

• Autorzy: Kazimierczak B. , Pijanowska D. G. , Baraniecka A. , Dawgul M. , Kruk J. , Torbicz W.

Identyfikatory

DOI

10.1016/j.bbe.2015.11.008

• Języki publikacji: EN

Abstrakty

EN

In this paper, the review of immunosensors for selected cardiovascular disease markers: human cardiac troponins and human C-reactive protein (CRP) is presented. In particular, (cTnI) amperometric immunosensor for cTnI measurements in the concentration range useful in medical diagnostics, based on the developed earlier human CRP amperometric immunosensor, is described. The human cTnI is recommended as one of specific myocardial damage biomarkers, and is considered as the ‘‘gold standard’’, whereas the human CRP is used as the powerful, nonspecific, supplementary biomarker of cardiovascular disease. Carbon, graphite and platinum pastes, used for fabrication of our immunosensor working electrode (WE), were investigated. In the developed simple measuring procedure, based on a direct solid phase enzyme-linked immunosorbent assay (ELISA), for the first time ascorbic acid monophosphate was used for cTnI detection as a substrate in enzymatic reaction of alkaline phosphatase labelling antibodies. Disposable amperometric graphite immunosen- sors, made on polyester film by means of microdispensing robot, suitable for determination of cTnI in the concentration range 0–35 μg/L with the sensitivity 0.67 μA/(μg/L) and linear correlation coefficient 0.91 were obtained.

Słowa kluczowe

EN

cardiac troponin I; human CRP; immunosensor; amperometry; ascorbic acid monophosphate

PL

troponina sercowa; CRP; immunoczujnik; amperometria

• Wydawca: Nałęcz Institute of Biocybernetics and Biomedical Engineering of the Polish Academy of Sciences ; Elsevier
• Czasopismo: Biocybernetics and Biomedical Engineering
• Rocznik: 2016
• Tom: Vol. 36, no. 1
• Strony: 29--41
• Opis fizyczny: Bibliogr. 107 poz., rys., tab., wykr.

Twórcy

autor

Kazimierczak B.

• Nałęcz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences, Trojdena 4, Warsaw 02-109, Poland

autor

Pijanowska D. G.

• Nałęcz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences, Trojdena 4, Warsaw 02-109, Poland; Institute of Electron Technology, Warsaw, Poland

autor

Baraniecka A.

• Nałęcz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences, Trojdena 4, Warsaw 02-109, Poland; Institute of Electron Technology, Warsaw, Poland

autor

Dawgul M.

• Nałęcz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences, Trojdena 4, Warsaw 02-109, Poland

autor

Kruk J.

• Nałęcz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences, Trojdena 4, Warsaw 02-109, Poland

autor

Torbicz W.

• Nałęcz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences, Trojdena 4, Warsaw 02-109, Poland

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Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.