Implementation of different separation techniques for resolving ternary mixture of Paracetamol, Pseudoephedrine Hydrochloride and Chlorpheniramine maleate with further quantification

• Autorzy: Farid Joliana F. , Mostafa Nadia M. , Essam Hebatallah M. , Fayez Yasmin M.

Identyfikatory

DOI

10.1556/1326.2021.00954

• Języki publikacji: EN

Abstrakty

EN

Due to the wide applicability of separation techniques that rely on the property of differential migration in pharmaceutical formulations analysis, different analytical strategies have been proposed to resolve mixtures of multi-components pharmaceuticals. Three separation methods were developed and validated for the simultaneous determination of Paracetamol (PAR), Pseudoephedrine HCl (PSE) and Chlorpheniramine maleate (CHP). The first method is a thin-layer chromatographic (TLC) separation, followed by densitometric measurement. The separation was carried out on aluminium sheet of silica gel 60 F₂₅₄ using ethanol:chloroform:ammonia (1:7:0.4, by volume) as the mobile phase. Determination of PAR, PSE and CHP was successfully applied over the concentration ranges of 3–25 µg/band, 0.5–10 µg/band and 0.1–6 µg/band, respectively. The second method is HPLC separation that was achieved on C18 column using the mobile phase acetonitrile:phosphate buffer pH 5 (10:90, v/v) at a flow rate 1 mL min⁻¹. PAR, PSE and CHP were determined by HPLC in concentration ranges of 5–400 μg mL⁻¹, 2–40 μg mL⁻¹ and 0.5–16 μg mL⁻¹, respectively. The third method is a capillary electrophoresis (CE) separation. The electrophoretic separation was achieved using 20 mM phosphate buffer (pH 6.5) at 20 kV. The linearity was reached over concentration ranges of 30–250 μg mL⁻¹, 5–50 μg mL⁻¹ and 0.8–20 μg mL⁻¹ for PAR, PSE and CHP, respectively. The developed methods were validated with respect to linearity, precision, accuracy and system suitability. The proposed methods were successfully applied for bulk powder and dosage form analysis with RSD of precision <2%. Moreover, statistical comparison with the official methods confirms the methods' validity.

Słowa kluczowe

EN

HPLC/TLC; capillary electrophoresis; Paracetamol; Pseudoephedrine HCl; Chlorpheniramine maleate

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2022
• Tom: Vol. 34, no. 3
• Strony: 351--360
• Opis fizyczny: Bibliogr. 13 poz., rys., tab., wykr.

Twórcy

autor

Farid Joliana F.

• Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El-Aini, 11562, Cairo, Egypt

• https://orcid.org/0000-0002-4888-1359

autor

Mostafa Nadia M.

• Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El-Aini, 11562, Cairo, Egypt

autor

Essam Hebatallah M.

• Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El-Aini, 11562, Cairo, Egypt

autor

Fayez Yasmin M.

• Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-El-Aini, 11562, Cairo, Egypt

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).