Simultaneous quantitative determination of active components in Tetrastigma hemsleyanum by RP-HPLC coupled with diode array detection

• Autorzy: Zhu Y. , Yin Q. , Du P. , Huang S. , Yang Y.

Identyfikatory

DOI

10.1556/1326.2017.00231

• Języki publikacji: EN

Abstrakty

EN

An effective, reliable, and sensitive reversed-phase high-performance liquid chromatography (RP-HPLC) with diode array detector (DAD) method was investigated for simultaneous determination of polydatin, isoquercitrin, resveratrol, and nicotiflorin in Tetrastigma hemsleyanum. The chromatographic separation of the four compounds was carried out on a Welchrom ODS column (4.6 mm × 250 mm, 5 μm) by gradient elution with phosphoric acid (H3PO4) aqueous solution (0.4%)–methanol as the mobile phase, at the temperature of 30 °C and a flow rate of 1.0 mL/min. The detection wavelength was set at 270 nm. Under optimum conditions, the baseline separation of these four compounds can be performed within 30 min. The developed method was validated in terms of detection limit, quantification limit, linearity, precision, and recovery tests. Eventually, the established HPLC–DAD method was successfully applied to the simultaneous determination of polydatin, isoquercitrin, resveratrol, and nicotiflorin in the extract of herb T. hemsleyanum.

Słowa kluczowe

EN

active component; Tetrastigma hemsleyanum; RP-HPLC; diode array detection

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2018
• Tom: Vol. 30, no. 3
• Strony: 186--190
• Opis fizyczny: Bibliogr. 26 poz., rys.

Twórcy

autor

Zhu Y.

• Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, Yunnan, China
• Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan, China
• Research Center for Analysis and Measurement, Kunming University of Science and Technology, Kunming 650093, China

autor

Yin Q.

• Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, Yunnan, China
• Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan, China

autor

Du P.

• Research Center for Analysis and Measurement, Kunming University of Science and Technology, Kunming 650093, China

autor

Huang S.

• Research Center for Analysis and Measurement, Kunming University of Science and Technology, Kunming 650093, China

autor

Yang Y.

• Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan, China

Bibliografia

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).