Effect of Lambda-Cyhalothrin - An Insecticide from the Group of Synthetic Pyrethroids - on the Concentrations of NF-ĸB and VEGFR2 in the Liver of Albino Swiss Mice as Markers of its Damage

Świerszcz, Łukasz; Roszkowska, Anna; Ruszel, Kinga; Wójciak-Czuła, Marta; Borzęcki, Andrzej; Nieradko-Iwanicka, Barbara; Siermontowski, Piotr

doi:10.2478/phr-2021-0011

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Effect of Lambda-Cyhalothrin - An Insecticide from the Group of Synthetic Pyrethroids - on the Concentrations of NF-ĸB and VEGFR2 in the Liver of Albino Swiss Mice as Markers of its Damage

Autorzy

Świerszcz Łukasz , Roszkowska Anna , Ruszel Kinga , Wójciak-Czuła Marta , Borzęcki Andrzej , Nieradko-Iwanicka Barbara , Siermontowski Piotr

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DOI

10.2478/phr-2021-0011

Warianty tytułu

Wpływ lambda-cyhalothrin - insektycydu z grupy syntetycznych pyretroidów - na stężenia NF-ĸB i VEGFR2 w wątrobie myszy Albino Swiss jako markerów jego uszkodzenia

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Abstrakty

Background: Lambda-cyhalothrin (LCH) is a one of the type II synthetic pyrethroids which is widely used in veterinary medicine and in agriculture to protect crops from pest insects. In previous studies, there are few reports about the influence of pyrethroids on the liver and its damage. Analyzing numerous publications, nuclear factor-ĸB (NF-ĸB) and vascular endothelial growth factor 2 (VEGFR2) seem to be sensitive indicators of microdamages occurring at the cellular level in the liver. The aim of the study was to investigate the effect of subacute poisoning with LCH on the concentration of NFĸB and VEGFR2 in the livers. Methods: The experiment was carried on 32 Albino Swiss mice (16 females and 16 males). The animals were divided into 4 groups. Controls received canola oil, the rest received LCH orally in oil at a dose of 2 mg/kg bw for 7 days. The NF-ĸB and VEGFR2 were mesuredin mice livers with ELISA kits. Results: The mean NF-ĸB concektration in control femals’ livers was 3.27ng/mL and after LCH it was 6.12ng/mL (p<0.05). In control males it was 5.49ng/mL and it did not significantly differ after LCH when it was 5.27ng/mL. The mean VEGFR2 in control females was 84.28ng/mL and after LCH it was 173.81ng/mL (p<0.05). In control males it was 170.61ng/mL and after LCH 170.06ng/mL. Conclusion:The NF-ĸB and VEGFR2 can be used as markers of liver damage after subacute poisoning with LCH on female mice. Females are more sensitive to LCH than males.

Wstęp: Lambda-cyhalotryna (LCH) jest jednym z syntetycznych pyretroidów typu II, szeroko stosowanym w weterynarii i rolnictwie do ochrony upraw przed owadami. We wcześniejszych badaniach niewiele jest doniesień o wpływie pyretroidów na wątrobę i jej uszkodzenia. Analizując liczne publikacje, czynnik jądrowy-ĸB (NF-ĸB) i czynnik wzrostu śródbłonka naczyniowego 2 (VEGFR2) wydają się być czułymi wskaźnikami mikrouszkodzeń występujących na poziomie komórkowym w wątrobie. Celem pracy było zbadanie wpływu podostrego zatrucia LCH na stężenie NFĸB i VEGFR2 w wątrobie. Metody: Doświadczenie przeprowadzono na 32 myszach Albino Swiss (16 samic i 16 samców). Zwierzęta podzielono na 4 grupy. Kontrolne grupy samic i samców otrzymywały olej rzepakowy, pozostałe otrzymywały LCH doustnie w oleju w dawce 2 mg/kg mc przez 7 dni. NF-ĸB i VEGFR2 były w wątrobach myszy były mierzone z użyciem zestawów ELISA. Wyniki: Średnie stężenie NF-ĸB w wątrobach kontrolnych samic wynosiło 3,27 ng/ml, a po LCH 6,12 ng/ml (p<0,05). U samców kontrolnych wynosił on 5,49 ng/ml i nie różnił się istotnie po LCH, gdzie wynosił 5,27 ng/ml. Średnie stężenie VEGFR2 u samic z grupy kontrolnej wynosił 84,28 ng/ml, a narażonych na LCH 173,81 ng/ml (p<0,05). U samców z grupy kontrolnej wynosiło 170,61 ng/ml, a narażonych na LCH 170,06 ng/ml. Wniosek: NF-ĸB i VEGFR2 można stosować jako markery uszkodzenia wątroby po podostrym zatruciu LCH u samic myszy. Samice są bardziej wrażliwe na LCH niż samce.

Słowa kluczowe

lambda-cyhalothrin nuclear factor ĸB vascular endothelial growth factor2 liver damage

lambda-cyhalotryna czynnik jądrowy ĸB czynnik wzrostu śródbłonka naczyniowego 2 uszkodzenie wątroby

Wydawca

Polskie Towarzystwo Medycyny i Techniki Hiperbarycznej

Czasopismo

Polish Hyperbaric Research

Rocznik

2021

Tom

nr 2(75)

Strony

57--68

Opis fizyczny

Bibliogr. 77 poz., tab.

Twórcy

autor

Świerszcz Łukasz

Department of Obstetrics and Perinatology in Lublin, Poland

autor

Roszkowska Anna

3rd Chair and Department of Gynecology in Lublin, Poland

autor

Ruszel Kinga

Students’ Scientific Association at The Chair and Department of Hygiene, Medical University in Lublin, Poland

autor

Wójciak-Czuła Marta

Team of Ophthalmology Departments, Mazowiecki Szpital Bródnowski in Warsaw, Poland

autor

Borzęcki Andrzej

Chair and Department of Hygiene and Epidemiology, Medical University in Lublin, Poland

autor

Nieradko-Iwanicka Barbara

barbara.nieradko-iwanicka@umlub.pl

Chair and Department of Hygiene and Epidemiology, Medical University in Lublin, Poland

autor

Siermontowski Piotr

Department of Underwater Works Technology, Naval Academy in Gdynia, Poland

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