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Densitometry, video-scanning and capillary electrophoresis for determination of valsartan and amlodipine in a combined dosage form: A comparative study

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Comparison of classical densitometry, video-scanning, and capillary electrophoresis was performed for determination of angiotensin II receptor antagonist, valsartan, and calcium channels blocker, amlodipine, in a combined dosage form. Thin layer chromatography was performed on RP8F254 TLC plates with a mobile phase consisting of acetonitrile-phosphate buffer at pH 9.0 (5:5, v/v) and temperature 20 °C. Densitometry was done in the reflectance mode at 217 nm for valsartan and in the absorbance mode at 370 nm for amlodipine. Video-scanning was elaborated at 254 and 366 nm for valsartan and amlodipine, respectively. For chromatographic analysis, calibration plots were constructed in the range of 0.4–2.8 μg per spot for valsartan and 0.02–0.14 μg per spot for amlodipine. Capillary electrophoresis (CE) was performed using a 75 μm × 94 cm fused silica capillary (72 cm effective length), 0.01 mol L-1 borate buffer at pH 8.0, 20 kV voltage, 30 °C temperature, hydrodynamic injection (10 mbar, 6 s) and UV detection at 237 nm. Calibration plots were constructed in the range of 0.1–0.6 mg mL-1 for valsartan and 0.005–0.03 mg mL-1 for amlodipine. All methods were validated in respect to robustness, specificity, stability, linearity, precision, and accuracy. Generally, statistical comparison between the methods did not show significant differences so all procedures are suitable for pharmaceutical analysis.
Rocznik
Strony
47--58
Opis fizyczny
Bibliogr. 12 poz., rys., tab.
Twórcy
autor
  • Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland
  • Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland
autor
  • Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland
autor
  • Medical University of Lublin Department of Medicinal Chemistry, Faculty of Pharmacy Jaczewskiego 4 20-090 Lublin Poland
Bibliografia
  • [1] C. Ferri, G. Croce and G. Desideri, Arch. Ther., 25, 300 (2008)
  • [2] N.K. Ramadan, H.M. Mohamed and A.A. Moustafa, Anal Lett. 43, 570 (2010)
  • [3] D. Kul, B. Dogan-Topal and S.A. Ozkan, J. AOAC Int. 93, 882 (2010)
  • [4] S.B. Patel, B.G. Chaudhari, M.K. Buch and A.B. Patel, Int. J. Chem. Tech. Res. 1, 1257 (2009)
  • [5] S.S. Chitlange, K. Bagri and D.M. Sakarkar, Asian J. Research Chem. 1, 15 (2008)
  • [6] A.V. Ramani, P. Sengupta and R. Mullangi, Biomed. Chromatogr. 23, 615 (2009)
  • [7] L. Kristofferson, E.L. Oiestad, M.S. Opdal, M. Krogh, E. Lundanes and A.S. Christophersen, J. Chromatogr. B 850, 147–160 (2007)
  • [8] S.R. Dhaneshwar, N.G. Patre and M.V. Mahadik, Chromatographia 69, 157 (2009)
  • [9] I. Vovk and M. Prošek, J. Chromatogr. A 768, 329 (2007)
  • [10] A.O. Alnajjar, J. AOAC Int. 94, 498 (2011)
  • [11] K.D. Altria, J. Chromatogr. A 856, 443 (1999)
  • [12] L. Geiser, S. Rudaz and J.L. Veuthey, Electrophoresis 24, 3049 (2003)
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-ba9038fe-3758-4a17-a3ad-1d97d85c3d47
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