A validated densitometric TLC method for analysis of cefuroxime axetil and potassium clavulanate in combined tablet dosage forms

• Autorzy: Sengar M. R , Gandhi S. V. , Patil U. P. , Rajmane V. S , Bothara K. G.
• Języki publikacji: EN

Abstrakty

EN

A simple, specific, accurate, and precise high-performance thin-layer chromatographic method for analysis of cefuroxime axetil and potassium clavulanate in a combined tablet dosage form is reported. The compounds were separated on aluminium foil plates precoated with silica gel 60 F 254 , with chloroform-methanol-toluene 4:3:3 ( v / v ) as mobile phase. Densitometric evaluation of the separated bands was performed at 225 nm. The two drugs were satisfactorily separated with R F 0.77 ± 0.0114 and 0.29 ± 0.0114 for cefuroxime axetil and potassium clavulanate, respectively. Response was a linear function of amount over the calibration ranges 500–2500 and 2000–10 000 ng per band, respectively. The method was successfully validated and used for analysis of the drugs in a pharmaceutical formulation. Recovery was 100.05 ± 0.98% for cefuroxime axetil and 99.94 ± 0.538% for potassium clavulanate (mean ± RSD).

Słowa kluczowe

EN

high-performance thin-layer chromatography; potassium clavulanate; cefuroxime axetil

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2010
• Tom: Vol. 22, no. 1
• Strony: 91--97
• Opis fizyczny: Bibliogr. 14 poz., rys., tab.

Twórcy

autor

Sengar M. R

• A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 India

autor

Gandhi S. V.

• A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 India

autor

Patil U. P.

• A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 India

autor

Rajmane V. S

• A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 India

autor

Bothara K. G.

• A.I.S.S.M.S. College of Pharmacy Department of Pharmaceutical Analysis Kennedy Road Pune 411001 India

Bibliografia

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