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Investigation of biosensor potential component stability caused by influence of external condition

Identyfikatory
Warianty tytułu
PL
Badanie wpływu warunków zewnętrznych na stabilność potencjalnego komponentu biosensorów
Języki publikacji
EN
Abstrakty
EN
The analysis of UV-VIS spectrum was used for testing changes through 36 days and the impact of external conditions (reduced temperature (R), microwave radiation (M) and normal conditions (NC) for comparisons) on the stability of: BSA and BSA-Fe2+ complexes after different treating procedure. The increase of BSA absorption with increasing concentration of Fe2+ was observed. Increased absorption of BSA, and BSA-Fe2+ with time, related probably to conformational changes present in the protein and changes in electrostatic reactions within the BSA-Fe2+ complexes. Examination of the impact of an external factor on stabilization of the protein and complexes showed a tendency to keep the stability under reduced temperature and a trend with accelerated protein and complex aging under microwave radiation. The observed tendencies to changes under the influence of external factors became more significant in time. These effects were most probably related to changes of the protein structure and time, while the observed trend of accelerating the changes was impacted by the applied external factors.
Słowa kluczowe
EN
biosensor   BSA   UV-Vis  
PL
biosensor   BSA   UV-Vis  
Rocznik
Strony
665--674
Opis fizyczny
Bibliogr. 49 poz., wykr., tab.
Twórcy
  • Department of Computer Science and Automatics, Faculty of Mechanical Engineering and Computer Science, University of Bielsko-Biala, ul. Willowa 2, 43-309 Bielsko-Biała, Poland
  • Department of Computer Science and Automatics, Faculty of Mechanical Engineering and Computer Science, University of Bielsko-Biala, ul. Willowa 2, 43-309 Bielsko-Biała, Poland
Bibliografia
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Uwagi
PL
Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-b8744f94-520a-491d-ae4b-f5030a7b108b
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