Heavy Metals Chelating Ability and Antioxidant Activity of Phragmites australis Stems Extracts

• Autorzy: Sellal Abdelhakim , Belattar Rima , Bouzidi Abdelouahab

Identyfikatory

DOI

10.12911/22998993/96276

• Języki publikacji: EN

Abstrakty

EN

In this study, the ability of hexane (HSE), chloroform (CSE), ethyl acetate (EASE) and methanolic (MSE) stems extracts from Phragmites australis or EDTA (as standard) to chelate iron using ferrozine method or zinc and copper using the murexide method is carried out in vitro. When the increased volumes of the HSE studied were taken from a stock solution of a fixed concentration 1 mg/ml at 25–175 μl for the iron and zinc chelating assay, 1–7 mg/ml for the copper chelating assay gave a significant (p≤ 0.01) activity. The obtained results showed that the HSE have the highest capacity to chelate ferrous ions below the EDTA (standard chelator) with absorbance arrive to the lesser extent 0.24±0.005, 0.04±0.013 which expresses 86% and 97% (compared to the control) of inhibition, respectively. For the murexide chelation, the results obtained also showed that the HSE and EDTA have a good (p≤ 0.01) chelating dose dependent effect towards zinc and copper ions with increased absorbance 0.45±0.02 and 0.42±0.02 with 54% and 56% of inhibition, respectively, for the zinc chelation and 0.66±0.03, 0.13±0.005 represent 44% and 88% for copper chelation. In contrast to the antioxidant capacity, the extract of hexane, ethyl acetate, chloroform and methanol from leaves, stems and roots of the Phragmites australis plant have a very low scavenger effect to the radical DPPH where the maximum inhibition is approximately 13.79%, obtained with the maximum volume. Finally, the HPLC analysis of effective extract (HSE) confirmed the presence of oxalic, citric, malic, succinic, fumaric, formic, acetic, propionic and butyric or iso butyric acid.

Słowa kluczowe

EN

chelation; Phragmites australis; common reed; DPPH; heavy metal

• Wydawca: Polskie Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology
• Czasopismo: Journal of Ecological Engineering
• Rocznik: 2019
• Tom: Vol. 20, nr 2
• Strony: 116--123
• Opis fizyczny: Bibliogr. 19 poz., rys., tab.

Twórcy

autor

Sellal Abdelhakim

• Department of biochemistry, Faculty of nature sciences and life, Ferhat Abbas University, Setif 1, Algeria

autor

Belattar Rima

• Department of Biology and Plant Ecology, Faculty of nature sciences and life, Ferhat Abbas University, Setif 1, Algeria

autor

Bouzidi Abdelouahab

• Department of biochemistry, Faculty of nature sciences and life, Ferhat Abbas University, Setif 1, Algeria

Bibliografia

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).