Exhaled breath analysis - quantifying the storage of lipophilic compounds in the human body

• Autorzy: Amann A. , King J. , Kupferthaler A. , Uterkofler K. , Koc H. , Teschl S. , Hinterhuber H.

Warianty tytułu

PL

Analiza wydechu - oznaczanie zawartości związków lipofilowych w organizmie człowieka

• Języki publikacji: EN

Abstrakty

EN

Real-time analysis of exhaled breath is a promising new method to get quantitative information on lipophilic compounds stored in the human body. Some pilot results are presented on isoprene, which is produced as a by-product of the cholesterol synthesis and appears in exhaled breath at concentrations of about 100 parts-per-billion (ppb). The results have been obtained using proton transfer reaction mass spectrometry (PTR-MS) from healthy volunteers undergoing an ergometer challenge test (with 75 W). Peak exhalation flow of isoprene is about 400 nmol/min shortly after start of the challenge. The setup holds great potential in capturing continuous dynamics of non-polar, low-soluble VOCs over a wide measurement range with simultaneous recording of physiological parameters affecting exhalation kinetics.

PL

Przedstawiono wstępne wyniki oznaczenia stężenia izoprenu w wydechu zdrowego człowieka. Izopren jest produktem ubocznym reakcji syntezy cholesterolu. Do oznaczenia zastosowano metodę reakcji przeniesienia protonu przy wykorzystaniu spektrometrii mas (PTR-MS).

Słowa kluczowe

EN

exhaled breath analysis; isoprene; proton transfer reaction mass spectrometry (PTR-MS)

PL

analiza wydechu człowieka; izopren; metoda reakcji przeniesienia protonu wraz ze spektrometrią mas (PTR-MS)

• Wydawca: Towarzystwo Chemii i Inżynierii Ekologicznej
• Czasopismo: Proceedings of ECOpole
• Rocznik: 2009
• Tom: Vol. 3, No. 1
• Strony: 9--13
• Opis fizyczny: Bibliogr. 4 poz., rys.

Twórcy

autor

Amann A.

• Innsbruck Medical University, Department of Operative Medicine, Anichstr. 35, A-6020 Innsbruck
• Breath Research Unit of the Austrian Academy of Sciences, Dammstr. 22, A-6850 Dornbirn

autor

King J.

• Innsbruck Medical University, Department of Operative Medicine, Anichstr. 35, A-6020 Innsbruck
• Breath Research Unit of the Austrian Academy of Sciences, Dammstr. 22, A-6850 Dornbirn
• Vorarlberg University of Applied Sciences, Hochschulstr. 1, A-6850 Dornbirn

autor

Kupferthaler A.

• Innsbruck Medical University, Department of Operative Medicine, Anichstr. 35, A-6020 Innsbruck
• Breath Research Unit of the Austrian Academy of Sciences, Dammstr. 22, A-6850 Dornbirn

autor

Uterkofler K.

• Breath Research Unit of the Austrian Academy of Sciences, Dammstr. 22, A-6850 Dornbirn
• Vorarlberg University of Applied Sciences, Hochschulstr. 1, A-6850 Dornbirn

autor

Koc H.

• Breath Research Unit of the Austrian Academy of Sciences, Dammstr. 22, A-6850 Dornbirn
• Vorarlberg University of Applied Sciences, Hochschulstr. 1, A-6850 Dornbirn

autor

Teschl S.

• University of Applied Sciences Technikum Wien, Höchstädtplatz, A-1120 Wien

autor

Hinterhuber H.

• Breath Research Unit of the Austrian Academy of Sciences, Dammstr. 22, A-6850 Dornbirn
• Innsbruck Medical University, Department of Psychiatry, Anichstr. 35, A-6020 Innsbruck

Bibliografia

• [1] Kushch I., Arendacka B., Stolc S., Mochalski P., Filipiak W., Schwarz K., Schwentner L., Schmid A., Dzien A., Lechleitner M., Witkovsky V., Miekisch W., Schubert J., Unterkofler K. and Amann A.: Breath isoprene - aspects of normal physiology related to age, gender and cholesterol profile as determined in a proton transfer reaction mass spectrometry study. Clin. Chem. Lab. Med., 2009, 47(5), 550-560.
• [2] Filser J.G., Csanady G.A., Denk B., Hartmann M., Kauffmann A., Kessler W., Kreuzer P.E., Putz C., Shen J.H. and Stei P.: Toxicokinetics of isoprene in rodents and humans. Toxicology, 1996, 113(1-3), 278-287.
• [3] West J.B.: Respiratory Physiology. The Essentials. 7th ed. Lippincott Williams & Wilkins, Baltimore 2005.
• [4] Mork A.K. and Johanson G.: A human physiological model describing acetone kinetics in blood and breath during various levels of physical exercise. Toxicol. Lett., 2006, 164(1), 6-15.