New multiplex PCR assays for estimating genetic diversity in rainbow trout (Oncorhynchus mykiss) by polymorphism of microsatellite DNA

• Autorzy: Kaczmarczyk D. , Kaczor A.
• Języki publikacji: EN

Abstrakty

EN

Multiplex PCR is a useful technique for estimating genetic diversity. This paper presents 3 new sets of primer pairs for effectively amplifying 10 microsatellite DNA loci from rainbow trout (Oncorhynchus mykiss). Unlike other sets of primer pairs that have been developed for amplifying rainbow trout microsatellite loci, ours do not require the hot-start PCR technique. In the paper, we describe the steps taken to choose the loci for each multiplex assay and to verify the genotyping results. We provide the compositions of the PCR mixture and the characteristics of the PCR thermal profile recommended for amplification.

Słowa kluczowe

EN

microsatellite DNA; multiplex PCR; population genetics; rainbow trout

PL

DNA mikrosatelitarne; genetyka populacyjna; pstrąg tęczowy

• Wydawca: University of Warmia and Mazury in Olsztyn, Poland
• Czasopismo: Environmental Biotechnology
• Rocznik: 2013
• Tom: Vol. 9, No. 1
• Strony: 19--24
• Opis fizyczny: Bibliogr. 14 poz., tab., wykr.

Twórcy

autor

Kaczmarczyk D.

• Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Słoneczna 45G, 10-718 Olsztyn, Poland; Phone: [phone]

autor

Kaczor A.

• Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Poland

Bibliografia

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