Simultaneous quantitation of aconitum alkaloids from You-Gui-yin in rat plasma by UPLC–ESI–MS and its application to a pharmacokinetic study

• Autorzy: Zhang JianHua , Wang Ling , Zhu Xi , Bai Xue , Yin Hua

Identyfikatory

DOI

10.1556/1326.2017.00316

• Języki publikacji: EN

Abstrakty

EN

You-Gui-Yin (YGY), a famous traditional Chinese medicine, has been widely used in clinics for the treatment of kidney-yang deficiency, yang deficiency caused by excessive yin, and osteoporosis. A rapid and sensitive ultraperformance liquid chromatography–electrospray ionization–mass spectrometry (UPLC–ESI–MS) method for simultaneous determination of six Aconitum alkaloids including aconitine (AC), hypaconitine (HA), mesaconitine (MA), benzoylaconine (BAC), benzoylhypaconine (BHA), and benzoylmesaconine (BMA) in rat plasma after oral administration of YGY was developed in this study. Chromatographic separation was performed on an ACQUITY UPLC™ BEH C18 column (2.1 × 100 mm, 1.7 μm) using gradient elution with the mobile phase consisting of 2 mmol/L ammonium formate in 0.05% formic acid aqueous solution and 0.05% formic acid methanol solution, at a flow rate of 0.20 mL/min. MS detection was performed in the positive ion mode. The calibration curves were linear in the concentration range of 0.04160–41.60 ng/mL, 0.1070–107.0 ng/mL, 0.07358–73.50 ng/mL, 0.03228–32.28 ng/mL, 0.01809–18.09 ng/mL, and 0.1320–132.0 ng/mL for AC, HA, MA, BAC, BHA, and BMA, respectively. The intra- and inter-day precisions (relative standard deviation [RSD]) were less than 11.6% and 12.6%, respectively. The accuracies Relative Error (RE) ranged from −10.2% to 5.6%, while the recoveries ranged from 70.4% to 99.3%. The method for simultaneous quantitation of Aconitum alkaloids of You-Gui-Yin in rat plasma is accurate and repeatable, and this method was successfully applied to investigate the pharmacokinetics of the six Aconitum alkaloids in rat plasma after oral administration of YGY. For the pharmacokinetic study, the pharmacokinetics of the six Aconitum alkaloids were best described by a two-compartment open model.

Słowa kluczowe

EN

UPLC–ESI–MS; Aconitum alkaloids; pharmacokinetics; You-Gui-Yin

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2019
• Tom: Vol. 31, no. 1
• Strony: 23--27
• Opis fizyczny: Bibliogr. 11 poz., rys., tab.

Twórcy

autor

Zhang JianHua

• The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China

autor

Wang Ling

• Standardization of Chinese Medicine Research Laboratory, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China

autor

Zhu Xi

• Standardization of Chinese Medicine Research Laboratory, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China

autor

Bai Xue

• Standardization of Chinese Medicine Research Laboratory, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China
• College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China

autor

Yin Hua

• Standardization of Chinese Medicine Research Laboratory, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China
• College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China

Bibliografia

• [1] Zhao, D. D.; Wang, J.; Cui, Y. J.; Wu, X. J. Tradit. Chin. Med. 2012, 32, 308.
• [2] Zhao, W. J.; Xiao, L. W.; Tong, P. J.; Zhou, H.; Pan, H.; Hu, Q. F. Chin. J. Trad. Med. Traum. Orthop. (Chin) 2000, 8, 9.
• [3] Wang, L. L. Nei. Mongol. J. Trad. Chin. Med. 2010, 29, 42.
• [4] Hikino, H.; Konno, C.; Takata, H.; Yamada, Y.; Yamada, C.; Ohizumi, Y.; Sugio, K.; Fujimura, H. J. Pharmacobiodyn. 1980, 3, 514.
• [5] Singhuber, J.; Zhu, M.; Prinz, S.; Kopp, B. J. Ethnopharmacol. 2009, 126, 18.
• [6] Gao, F.; Li, Y. Y.; Wang, D.; Huang, X.; Liu, Q. Molecules 2011, 17, 5187.
• [7] Xiao, P. G.; Wang, F. P.; Gao, F.; Yan, L. P.; Chen, D. L.; Liu, Y. Acta Phytotaxon. Sin. 2006, 44, 1.
• [8] Yang, Y.; Chen, J.; Shi, Y. P. J. Chromatogr. B Biomed. Sci. Appl. 2010, 878, 2811.
• [9] Wang, X. Z.; Li, X. W.; Li, L. J.; Li, M.; Liu, Y.; Wu, Q.; Li, P.; Jin, Y. R. J. Sep. Sci. 2016 39, 1971.
• [10] Liu, J. J.; Li, Q.; Yin, Y. D.; Liu, R.; Xu, H. R.; Bi, K. S. J. Sep. Sci. 2014, 37, 171.
• [11] Godfrey, K. R.; Arundel, P. A.; Dong, Z. M.; Bryant, R. Comput. Methods Programs Biomed. 2011, 104, 62.

Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).