Simultaneous analysis of phenylephrine hydrochloride, guaiphenesin, ambroxol hydrochloride, and salbutamol (as salbutamol sulphate) by use of a validated high-performance liquid chromatographic method

• Autorzy: Joshi S , Bhatia C , Bal C. S , Rawat M. S. M
• Języki publikacji: EN

Abstrakty

EN

A simple, reversed-phase HPLC method has been developed for rapid, simultaneous quantification of phenylephrine hydrochloride, guaiphenesin, ambroxol hydrochloride, and salbutamol (as salbutamol sulphate) in a commercial cough-cold liquid formulation. The compounds were separated on a 250 mm × 4.6 mm C8 column with a gradient prepared from pH 3.0 phosphate buffer and 1:1 methanol-acetonitrile as mobile phase at a flow rate of 1.0 mL min ^-1. Elution of the analytes was achieved in less than 15 min. Detection was by UV absorbance at 273 nm for phenylephrine hydrochloride and guaiphenesin and 225 nm for ambroxol hydrochloride and salbutamol. Percentage recovery and RSD were, respectively, 100.09% and 0.22% for phenylephrine hydrochloride, 100.43% and 0.50% for guaiphenesin, 100.91% and 0.70% for ambroxol hydrochloride, and 100.54% and 0.55% for salbutamol. The components of the syrup formulation were quantified on the basis of the peak areas obtained from freshly prepared standard solutions. The method was validated in accordance with ICH guidelines.

Słowa kluczowe

EN

simultaneous quantification; validation; cough-cold syrup; RP-HPLC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2011
• Tom: Vol. 23, no. 1
• Strony: 109--119
• Opis fizyczny: Bibliogr. 18 poz., rys., tab.

Twórcy

autor

Joshi S

• K.L.D.A.V. (P.G.) College Department of Chemistry Roorkee, Uttarakhand India 247667

autor

Bhatia C

• K.L.D.A.V. (P.G.) College Department of Chemistry Roorkee, Uttarakhand India 247667

autor

Bal C. S

• Talwar Pharma Jhabrera Road Manglore, Roorkee, Uttarakhand India 247656

autor

Rawat M. S. M

• H.N.B. Garhwal University Department of Chemistry Srinagar, Uttarakhand India

Bibliografia

• [1] R. Bhushan, S. Joshi, M. Arora, and M. Gupta, J. Planar Chromatogr., 18, 164 (2005)
• [2] S. Joshi, A. Sharma, C.S. Bal, and M.S.M. Rawat, Asian J. Pharm., 3, 274–277 (2009)
• [3] W.E. Serafin, Drugs used in the treatment of asthma, in: P.B. Molinof and R.W. Ruddon (eds), The Goodman & Gilman’s pharmacological basis of therapeutics, McGraw-Hill, New York, 9th edn, 1996
• [4] B. Olmo, A. Garcia, A. Marin, and C. Barbas, J. Chromatogr. B, 817, 159 (2005)
• [5] A.E. Gindy, S. Emara, and A. Mostafa, J. Pharm. Biomed. Anal., 41, 421 (2006)
• [6] A.E. Gindy, S. Emara, and H. Shaaban, J. Pharm. Biomed. Anal., 43, 973 (2007)
• [7] M.S. Ali, M. Ghori, S. Rafiuddin, and A.R. Khatri, J. Pharm. Biomed. Anal., 43, 158 (2007)
• [8] G.M. Hadad, A.E. Gindy, and W.M.M. Mahmoud, Spectrochim. Acta, 70, 655 (2008)
• [9] S.M. Amer, S.S. Abbas, M.A. Shehata, and N.M. Ali, J. AOAC Int., 91, 27 (2008)
• [10] İ.M. Palabıyık and F. Onur, Chromatographia, 66, 93 (2007)
• [11] M.R. Louhaichi, S. Jebali, M.H. Loueslati, N. Adhoum, and L. Monser, Talanta, 78, 991 (2009)
• [12] X. Xu, Xiao Lui, and J.T. Stewart, J. Liq. Chromatogr. Relat. Technol., 23, 1 (2000)
• [13] Y. Dong, X. Chen, Y. Chen, and Z. Hu, J. Pharm. Biomed. Anal., 39, 285 (2005)
• [14] E. Mario, L.G. Meehan, J. Pharm. Sci., 59, 538 (2006)
• [15] H.N. Dave, R.C. Mashru, and A.R. Thakkar, Anal. Chim. Acta, 597, 113 (2007)
• [16] International Conference on Harmonization on Technical Requirements for Registration of Pharmaceuticals for Human Use, Validation of Analytical Procedures: Text and Methodology Q2 (R1), Geneva, 2005
• [17] P.D. Sethi, High Performance Liquid Chromatography, CBS Publishers and Distributors, New Delhi, 2001, pp. 105–109
• [18] L.R. Snyder, J.L. Glajch, and J. Kirkland, Practical HPLC Method Development, Wiley-Interscience Publication, New York, 1988, pp. 126–127