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Impedance Sensors Made in PCB and LTCC Technologies for Monitoring Growth and Degradation of Pseudomonal Biofilm

Treść / Zawartość
Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The suitability of low-cost impedance sensors for microbiological purposes and biofilm growth monitoring was evaluated. The sensors with interdigitated electrodes were fabricated in PCB and LTCC technologies. The electrodes were golden (LTCC) or gold-plated (PCB) to provide surface stability. The sensors were used for monitoring growth and degradation of the reference ATCC 15442 Pseudomonas aeruginosa strain biofilm in invitro setting. During the experiment, the impedance spectra of the sensors were measured and analysed using electrical equivalent circuit (EEC) modelling. Additionally, the process of adhesion and growth of bacteria on a sensor’s surface was assessed by means of the optical and SEM microscopy. EEC and SEM microscopic analysis revealed that the gold layer on copper electrodes was not tight, making the PCB sensors susceptible to corrosion while the LTCC sensors had good surface stability. It turned out that the LTCC sensors are suitable for monitoring pseudomonal biofilm and the PCB sensors are good detectors of ongoing stages of biofilm formation.
Rocznik
Strony
369--380
Opis fizyczny
Bibliogr. 33 poz., fot., rys., tab., wykr.
Twórcy
autor
  • Wrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, Z. Janiszewskiego 11/17, 50-372 Wrocław, Poland
autor
  • Medical University of Wrocław, Department of Pharmaceutical Microbiology and Parasitology, Borowska 211a, 50-556 Wrocław, Poland
autor
  • Wrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, Z. Janiszewskiego 11/17, 50-372 Wrocław, Poland
autor
  • Wrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, Z. Janiszewskiego 11/17, 50-372 Wrocław, Poland
autor
  • Wrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, Z. Janiszewskiego 11/17, 50-372 Wrocław, Poland
autor
  • Medical University of Wrocław, Department of Pharmaceutical Microbiology and Parasitology, Borowska 211a, 50-556 Wrocław, Poland
  • Medical University of Wrocław, Department of Pharmaceutical Microbiology and Parasitology, Borowska 211a, 50-556 Wrocław, Poland
autor
  • Wrocław University of Science and Technology, Faculty of Microsystem Electronics and Photonics, Z. Janiszewskiego 11/17, 50-372 Wrocław, Poland
autor
  • Wrocław University of Science and Technology Centre for Advanced Manufacturing Technologies, I. Łukasiewicza 5, 50-371 Wrocław, Poland
Bibliografia
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  • [28] Zheng, L.Y., Congdon, R.B., Sadik, O.A., Marques, C.N.H., Davies, D.G., Sammakia, B.G., Turner, J.N. (2013). Electrochemical measurements of biofilm development using polypyrrole enhanced flexible sensors, Sensor. Actuat. B-Chem., 182, 725-732.
  • [29] Munoz-Berbel, X., Garcia-Aljaro, C., Munoz, F.J. (2008). Impedimetric approach for monitoring the formation of biofilms on metallic surfaces and the subsequent application to the detection of bacteriophages, Electrochim. Acta, 53, 5739-5744.
  • [30] Piasecki, T., Chabowski, K., Nitsch, K. (2016). Design, calibration and tests of versatile low frequency impedance analyser based on ARM microcontroller. Measurement, 91, 155-161.
  • [31] Piasecki, T. (2015). Fast impedance measurements at very low frequencies using curve fitting algorithms. Meas. Sci. Technol., 26, 065002.
  • [32] Babauta, J.T., Beyenal, H. (2014). Mass transfer studies of Geobacter sulfurreducens biofilms on rotating disk electrodes, Biotechnol. Bioeng., 111, 285-294.
  • [33] Bozkurt, A., Lal, A. (2011). Low-cost flexible printed circuit technology based microelectrode array for extracellular stimulation of the invertebrate locomotory system. Sensor. Actuat. A-Phys., 169, 89-97.
Uwagi
EN
This work was supported by Wrocław University of Technology statutory grant.
PL
Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-b517bc96-7013-4690-97e8-d259731c7b24
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