Determination of glyphosate and glufosinate in human serum by monospin TiO extraction and liquid chromatography–tandem mass spectrometry

• Autorzy: Saito Takeshi , Namera Akira , Tsuji Tomoatsu , Noguchi Wataru , Inokuchi Sadaki

Identyfikatory

DOI

10.1556/1326.2018.00513

• Języki publikacji: EN

Abstrakty

EN

We developed and validated an assay for determination of glyphosate (GLYP) and glufosinate (GLUF) in human serum. Serum samples were extracted by using a MonoSpin® TiO column and analyzed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). MonoSpin® TiO tends to specifically bind to phosphate groups. The assay was linear over a concentration range of 1–250 μg/mL. The recoveries for the 2 compounds were 1.6%–2.3%. The intra- and inter-day variations were <15%. Precision and accuracy were 5.6%–12.7% and 97.0%–103.9%, respectively. The validated method was applied to quantify the GLYP and GLUF content in the serum of GLYP and GLUF-poisoned patients. In conclusion, the method was successfully applied for accurate determination of GLYP and GLUF in serum obtained from patients with GLYP and GLUF poisoning.

Słowa kluczowe

EN

glyphosate; glufosinate; MonoSpin TiO kolumn; serum

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2020
• Tom: Vol. 32, no. 1
• Strony: 10--15
• Opis fizyczny: Bibliogr. 19 poz., rys., tab.

Twórcy

autor

Saito Takeshi

• Tokai University School of Medicine, Isehara, Kanagawa 259-1143, Japan

autor

Namera Akira

• Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8553, Japan

autor

Tsuji Tomoatsu

• Tokai University School of Medicine, Isehara, Kanagawa 259-1143, Japan

autor

Noguchi Wataru

• Department of Emergency, Kagoshima City Hospital, Uearata, Kagoshima, Kagoshima 890 8760, Japan

autor

Inokuchi Sadaki

• Tokai University School of Medicine, Isehara, Kanagawa 259-1143, Japan

Bibliografia

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Uwagi

PL

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).