Biodegradable and antimicrobial polycaprolactone nanofibers with and without silver precipitates

• Autorzy: Dobrzański L. A. , Hudecki A. , Chladek G. , Król W. , Mertas A.
• Języki publikacji: EN

Abstrakty

EN

Purpose: The purpose of the paper is to present the results of own researches, including the study of the structure and the properties of new obtained single- and doublecomponent polycaprolactone polymer nanofibers as well as of composite nanofibers with and without silver precipitates produced by electrospinning including the results of biological research, proving the usefulness of the newly developed nano-engineering materials and their applicability in regenerative medicine, as well as tissue engineering. Design/methodology/approach: On the basis of the data available from the fundamental literature and based on the criteria of potential and attractiveness, polycaprolactone was selected for research from among a number of polymer materials, using a method of procedural benchmarking and weighted scores. The obtained nanomaterials undergone the following examinations to confirm the assumed aim of the work: infrared spectroscopy FTIR, Wide-angle X-ray scattering (WAXS), BET, Langmuir specific surface area and DTF porosity assessed with the gas adsorption method, in a transmission electron microscope (TEM), a scanning electron microscope (SEM), a fluorescence microscope, antibacterialness and antifungalness investigations and examinations of biological properties in vitro. Findings: The applicability of polymer fibers in medicine depends on biocompatibility and non-toxicity of the applied material, which is influenced by the chemical purity of the materials applied and the toxicity of the input solvents. The potential toxicity of nanofibers should therefore be eliminated, starting with selection of materials used for obtaining solutions. Many other factors fundamental for the quality and properties of polycaprolactone nanofibers need to be taken into account to create single- and doublecomponent and composite nanofibers. Practical implications: The obtained composite materials, due to their non-toxicity resulting from the components applied, including solvents, bacteriocidity and bioactivity, may find their applications in tissue engineering as membranes in controlled regeneration of bone tissue, as carriers of medicinal agents in bone surgery, as implantable surgical meshes and as scaffolds for a tissue culture. In turn, the composite core-shell nanofibers, by combining the antibacterial properties of the coating with bioactive properties of the core, are attractive materials for three-dimensional tissue scaffold. Such materials can be used as a carrier of medicine, a treatment of hard healing wounds, invasive surgery, neurosurgery, as substrate for the culturing of a retina, material to reconstruct nerves and in dentistry or oncology, to replace the natural tissue removed because of a cancer with the possibility of applying a therapeutic agent, e.g., an antibiotic or a medicine used in cancer therapies, released after the dissolution of the coating of nanofibers. Originality/value: The present paper is the original report from a personal own research and explains the concept and scope of own research of a new obtained single- and doublecomponent polycaprolactone polymer nanofibers as well as of composite nanofibers produced by electrospinning for application in regenerative medicine, the presentation of technological conditions and methodology of own research into polymer nanofibers, and above all very detailed description of the results of own investigations

Słowa kluczowe

EN

nanocomposite; polymer nanofibers; electrospinning; biocompatibility; non-toxicity; regenerative medicine; tissue engineering

PL

nanokompozyt; nanowłókna polimerowe; elektroprzędzenie; biozgodność; nietoksyczność; medycyna regeneracyjna; inżynieria tkankowa

• Wydawca: International OCSCO World Press
• Czasopismo: Archives of Materials Science and Engineering
• Rocznik: 2015
• Tom: Vol. 76, nr 1
• Strony: 5--26
• Opis fizyczny: Bibliogr. 30 poz.

Twórcy

autor

Dobrzański L. A.

• Institute of Engineering Materials and Biomaterials, Silesian University of Technology, ul. Konarskiego 18a, 44-100 Gliwice, Poland

autor

Hudecki A.

• Institute of Engineering Materials and Biomaterials, Silesian University of Technology, ul. Konarskiego 18a, 44-100 Gliwice, Poland

autor

Chladek G.

• Institute of Engineering Materials and Biomaterials, Silesian University of Technology, ul. Konarskiego 18a, 44-100 Gliwice, Poland

autor

Król W.

• Department of Microbiology and Immunology, Medical University of Silesia in Katowice, ul. Jordana 19, 41-808 Zabrze, Poland

autor

Mertas A.

• Department of Microbiology and Immunology, Medical University of Silesia in Katowice, ul. Jordana 19, 41-808 Zabrze, Poland

Bibliografia

• [1] L.A. Dobrzański, Overview and general ideas of the development of constructions, materials, technologies and clinical applications of scaffolds engineering for regenerative medicine, Archives of Materials Science and Engineering 69/2 (2014) 53-80.
• [2] L.A. Dobrzański, Obtaining of porous high-strength engineering materials for scaffolds assuring the synergy of classical prosthetics/implantation and tissue engineering methods, 2015, unpublished work.
• [3] L.A. Dobrzański, M Pawlyta, A. Hudecki, Conceptual study on a new generation of the high-innovative advanced porous and composite nanostructural functional materials with nanofibers. Journal of Achievements in Materials and Manufacturing Engineering 49/2 (2011) 550-565.
• [4] L.A. Dobrzański (ed.), My links with medicine, a book prepared for publishing.
• [5] L.A. Dobrzański et al., Determining the importance of the effect of the onedimensional nanostructural materials on the structure and properties of newly developed functional nanocomposite and nanoporous materials, Project DEC-2012/07/B/ST8/04070, Gliwice, 2013-2016.
• [6] A. Hudecki (Advisor L.A. Dobrzański), Composite nanofibers with a bioactive core and an antibacterial coating on the tissue scaffold, PhD theses, Silesian University of Technology; Gliwice: 2015 (in Polish).
• [7] A. Arsiwala, P. Desai, V. Patravale, Recent advances in micro/nanoscale biomedical implants, Journal of Controlled Release 189 (2014) 25-45.
• [8] D. Lambrick, World Artificial Organs Market by 2017, MarketandMarkets, 2014, Available from: http://www.powershow.com/view0/608c4e-YjNiM/ World_Artificial_Organs_Market_by_2017_powerpoi nt_ppt_presentation, Access in: 15.09.2015.
• [9] P. Zorlutuna, N. Annabi, G. Camci-Unal, M. Nikkhah, J.M. Cha, J.W. Nichol, A. Manbachi, H. Bae, S. Chen, A. Khademhosseini, Microfabricated Biomaterials for Engineering 3D Tissues, Advanced Materials 24/14 (2012) 1782-1804.
• [10] F. Yang, W.L. Neeley, M.J. Moore, J.M. Karp, A. Shukla, R. Langer, Tissue Engineering: The Therapeutic Strategy of the Twenty-First Century, in: C.T. Laurencin, L.S. Nair (eds.), Nanotechnology and Tissue Engineering: The Scaffold, CRC Press Taylor & Francis Group, Boca Raton, FL, 2008, 3-32.
• [11] Y.C. Fung, A Proposal to the National Science Foundation for An Engineering Research Center at UCSD, Center for the Engineering of Living Tissues, UCSD #865023, 2001.
• [12] R.P. Lanza, R. Langer, J. Vacanti (eds.), Principles of Tissue Engineering. San Diego: Academic Press, 2000.
• [13] A. Atala, R.P. Lanza (eds.), Methods of Tissue Engineering, Academic Press, San Diego, 2002.
• [14] J. Kay, P.D.J. Weitzman (eds.), Krebs' citric acid cycle: half a century and still turning, Biochemical Society Symposium 54, The Biochemical Society, London, 1987.
• [15] L.A. Dobrzański, A. Hudecki, Structure, geometrical characteristics and properties of biodegradable microand polycaprolactone nanofibers, Archives of Materials Science and Engineering 70/1 (2014) 5-13.
• [16] L.A. Dobrzański, A. Hudecki, G. Chladek, W. Król, A. Mertas, Surface properties and antimicrobial activity of composite nanofibers of polycaprolactone with silver precipitations. Archives of Materials Science and Engineering 70/2 (2014) 53-60.
• [17] L.A. Dobrzański (ed.). Polymer nanofibers produced by electrospinning applied in regenerative medicine, Open Access Library V/3 (2015) 1-168.
• [18] L.A. Dobrzański, E. Hajduczek, A. Hudecki, Materials Challenges in Regenerative Medicine, in: L.A. Dobrzański (ed.), Polymer nanofibers produced by electrospinning applied in regenerative medicine, Open Access Library V/3 (2015) 12-82.
• [19] L.A. Dobrzański, A. Hudecki, Polymer Nanofibers Materials, Fabrication Technologies and Research Methods, in: L.A. Dobrzański (ed.), Polymer nanofibers produced by electrospinning applied in regenerative medicine, Open Access Library V/3 (2015) 83-126.
• [20] L.A. Dobrzański, A. Hudecki, Polymer Nanofibers Applied in Regenerative Medicine, in: L.A. Dobrzański (ed.). Polymer nanofibers produced by electrospinning applied in regenerative medicine, Open Access Library V/3 (2015) 127-168.
• [21] L.A. Dobrzański, A. Hudecki, A way of producing composite material with bioactive and bactericidal properties, Patent application P 410427, 08.12.2014.
• [22] L.A. Dobrzański, A. Hudecki, A way of producing composite material with bioactive and bactericidal properties, Patent application P 410452, 08.12.2014.
• [23] L.A. Dobrzański, A. Hudecki, G. Chladek, W. Król, A. Mertas, Biodegradable and antimicrobial polycaprolactone nanofibers with and without silver precipitates, XXIV International Materials Research Congress, IMRC’2015, Cancun, Mexico; 2015.
• [24] L.A. Dobrzański, A. Hudecki, G. Chladek, W. Król, A. Mertas, Fabrication, Structure and Properties of the Electrospinned Nanofibers and Nanocomposites for Medical Applications, BIT’s 5 th Annual World Congress of Nano Science and Technology on “Small Size, Big World”, Nano S&T-2015, Xi’an, China, 2015.
• [25] L.A. Dobrzański, Advanced electron microscopy methods aiding the development of surface engineering of materials, World Congress on Microscopy: Instrumentation, Techniques and Applications in Life Sciences and Materials Sciences, WCM 2015, Kottayam, India, 2015.
• [26] L.A. Dobrzański, A. Hudecki, G. Chladek, W. Król, A. Mertas, Electrospinned nanofibers and nanocomposites for regenerative medicine, BIT’s 6th Annual World Gene Convention-2015 on “More Advanced, More Healthy and More Safety”, WGC-2015, Qingdao, China, 2015.
• [27] L.A. Dobrzański, A. Hudecki, Composite nanofibers with bioactive core and antibacterial coating for tissue scaffolds produced by electrospinning, 22nd International Scientific Conference on "Achievements in Mechanical and Materials Engineering", AMME’2015, Zakopane, 2015.
• [28] A.D. Dobrzańska-Danikiewicz, Foresight methods for technology validation, roadmapping and development in the surface engineering area, Archives of Materials Science Engineering 44/2 (2010) 69-86.
• [29] A.D. Dobrzańska-Danikiewicz, Computer integrated development prediction methodology in materials surface engineering, Open Access Library 1/7 (2012) 1-289 (in Polish).
• [30] European Commission, Press Announcement, Towards a Strategy on Serious Road Traffic Injuries – Frequently asked questions, Brussels, European Union, 19 March 2013, Available from: http://europa.eu/rapid/press-release_MEMO-13-232_ en.htm, Access in: 15.09.2015.