Determination of the binding mechanism of cobalt(II) meso-tetraphenyl porphyrin with plant-esterase

• Autorzy: Liu Zishan , Dong Liang , Li Feifeng , Hou Changjun , He Kun , Huo Danqun

Identyfikatory

DOI

10.2478/pjct-2021-0004

• Języki publikacji: EN

Abstrakty

EN

Plant-esterase (EC 3.1.1.X) has received much attention because plant esterase and acetylcholinesterase (AChE) share a similar sensitivity towards organophosphorus (OP) pesticides detection with the same inhibition mechanism. To improve the analytical performance, tetraphenyl metal porphyrin, as an indicator was introduced to combine with plant-esterase. The time of reach equilibrium in PBS solution was shortened after adding plant-esterase by assaying the intensify change of the porphyrin spectrum. Meanwhile, intensify of porphyrin spectrum with plant-esterase was increased compared with that of only the porphyrin spectrum in solution. Tetraphenyl metal porphyrin, such as cobalt(II) meso-tetraphenyl porphyrin, is a mixed reversible inhibitor of plant-esterase from kinetic parameters. The combination ratio of plant-esterase and porphyrin is 2:1. On the other hand, the interaction between CoTPPCl and plant-esterase is the strongest among all tested tetraphenyl metal porphyrin. And the mixed system (CoTPPCl-plant-esterase) showed the best sensitivity towards the tested pesticide. All these results indicated that a complex system composed of tetraphenyl metal porphyrin and plant-esterase was fit for detecting pesticides. They make meaningful guidance on the further design of sensing material in monitoring pesticides.

Słowa kluczowe

EN

plant-esterasete; traphenyl metal porphyrin; binding mechanism

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2021
• Tom: Vol. 23, nr 1
• Strony: 25--30
• Opis fizyczny: Bibliogr. 12 poz., rys., tab.

Twórcy

autor

Liu Zishan

• Sichuan University of Science & Engineering Zigong, China
• Key Laboratory of Biorheology Science and Technology Ministry of Education, College of Bioengineering, Chongqing University Chongqing, China

autor

Dong Liang

• Sichuan University of Science & Engineering Zigong, China

autor

Li Feifeng

• Sichuan University of Science & Engineering Zigong, China

autor

Hou Changjun

• Key Laboratory of Biorheology Science and Technology Ministry of Education, College of Bioengineering, Chongqing University Chongqing, China

autor

He Kun

• Key Laboratory of Biorheology Science and Technology Ministry of Education, College of Bioengineering, Chongqing University Chongqing, China

autor

Huo Danqun

• Key Laboratory of Biorheology Science and Technology Ministry of Education, College of Bioengineering, Chongqing University Chongqing, China

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2021).