Ultrasonic-assisted extraction process and method validation for deoxypodophyllotoxin from the roots of Anthriscus sylvestris: Application of response surface methodology and UPLC–PDA–QDa

• Autorzy: Kim Hyo Seon , Lee A Yeong , Moon Byeong Cheol , Kim Wook Jin , Choi Goya

Identyfikatory

DOI

10.1556/1326.2018.00397

• Języki publikacji: EN

Abstrakty

EN

Deoxypodophyllotoxin (DPT), or anthricin, is a lignan isolated from the roots of Anthriscus sylvestris and is reported to exhibit anti-inflammatory, anti-oxidant, and anti-asthmatic effects. Herein, the conditions for the extraction of DPT from A. sylvestris are optimized using a Box–Behnken design (BBD) method based on response surface methodology (RSM). DPT was detected by ultra-performance liquid chromatography coupled with photodiode array and quadrupole detector (UPLC–PDA–QDa) and analytical validation methods based on International Conference on Harmonization (ICH) guidelines. In preliminary experiments, the experimental conditions of extraction time, solvent percentage, and temperature were selected for optimization. The adequacy of the experimental model was statistically evaluated, and the regression coefficient (R²), adjusted regression coefficient (R²adjust), and p-value of the lack-of-fit were determined as 97.86%, 94.02%, and 0.124, respectively. The maximum yield of DPT was estimated to be 2.341 mg/g for 30 min in 100% methanol at 60 °C, and the actual yield was measured as 2.295 mg/g (±0.023) under the same conditions.

Słowa kluczowe

EN

Anthriscus sylvestris; deoxypodophyllotoxin; optimization; response surface methodology; UPLC–PDA–QDa

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2019
• Tom: Vol. 31, no. 2
• Strony: 126--132
• Opis fizyczny: Bibliogr. 24 poz., rys., tab.

Twórcy

autor

Kim Hyo Seon

• K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Republic of Korea

autor

Lee A Yeong

• K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Republic of Korea

autor

Moon Byeong Cheol

• K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Republic of Korea

autor

Kim Wook Jin

• K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Republic of Korea

autor

Choi Goya

• K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Republic of Korea

Bibliografia

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).