New long-term action insulin formulations obtained using polycations for heparin neutralization

• Autorzy: Kamiński Kamil , Kaczor-Kamińska Marta , Irska Izabela , Popiołek Iwona , Szczubiałka Krzysztof , Nowakowska Maria

Identyfikatory

DOI

10.1515/bams-2019-0029

• Języki publikacji: EN

Abstrakty

EN

Phenomena that occur between an insulin and four different positively charged polymers (protamine, cationic dextran, chitosan, and poliallylamine derivatives) were studied by dynamic light scattering and fluorescence measurements (using fluorescein-labeled polymers). These processes were compared to the reaction of polycations with heparin that is responsible for the neutralization of anticoagulant activity in blood stream.The nature of polycations interaction with heparin is electrostatic, while the interaction with insulin is more complicated.We observed that the presence of zinc atoms (and its complexing by nitrogen from macromolecules) is critical for insulin suspensions formation and stability. The differences between the nature of these two reactions were revealed. The highly immunogenic action of protamine present in long-acting insulin products makes it reasonable to develop similar systems based on the nonprotein polycations.

Słowa kluczowe

EN

heparin; insoluble insulin; polycations; protamine

• Wydawca: Uniwersytet Jagielloński - Collegium Medicum ; Index Copernicus Sp. z o.o.
• Czasopismo: Bio-Algorithms and Med-Systems
• Rocznik: 2019
• Tom: Vol. 15, no. 3
• Strony: art. no. 20190029
• Opis fizyczny: Bibliogr. 29 poz., rys.

Twórcy

autor

Kamiński Kamil

• Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

autor

Kaczor-Kamińska Marta

• Chair of Medical Biochemistry, Jagiellonian University, Collegium Medicum, Kopernika 7C, 31-034, Krakow, Poland

autor

Irska Izabela

• Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

autor

Popiołek Iwona

• Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

autor

Szczubiałka Krzysztof

• Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

autor

Nowakowska Maria

• Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).