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Differences in populations of filamentous bacteria involved in foaming and bulking of activated sludge

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Bulking and foaming of activated sludge are related to excessive proliferation of a specific group of activated sludge biocenosis – filamentous bacteria. The research was carried out to compare filamentous bacteria populations in foam and activated sludge in a full-scale municipal wastewater treatment plant located near Warsaw (Poland). Fluorescent in situ hybridization (FISH) – a quantitative, culture-independent, molecular method was applied to evaluate the structure of filamentous populations. Activated sludge and foam were examined for the abundance of eleven groups of these microorganisms, which occur in wastewater treatment plants in Europe. Filamentous bacteria constituted 18% and 24% of all bacteria detected in sludge and foam, respectively. The structures of filamentous bacteria populations in sludge and foam were different, although the same bacteria were found in both types of samples. The most abundant filaments belonged to phylum Chloroflexi (targeted by the CFXmix probe) and genus Microthrix (targeted by the MPAmix probe) in sludge and foam, respectively. The third significantly abundant bacteria was Haliscomenobacter hydrossis (targeted by HHY654).
Rocznik
Strony
30--33
Opis fizyczny
Bibliogr. 22 poz., wykr.
Twórcy
  • Warsaw University of Technology, Department of Biology Faculty of Environmental Engineering, Nowowiejska 20, 00-653 Warsaw, Poland
  • Warsaw University of Technology, Department of Biology Faculty of Environmental Engineering, Nowowiejska 20, 00-653 Warsaw, Poland
Bibliografia
  • [1] Nielsen, P. H., Mielczarek, A. T., Kragelund, C., Nielsen, J. L., Saunders, A. M., Kong, Y., Hansen, A. A., Vollertsen J. “A conceptual ecosystem model of microbial communities in enhanced biological phosphorus removal plants.” Water research, 44(17) (2010): 5070 – 5088.
  • [2] Eikelboom D.H. “Process control of activated sludge plants by microscopic investigation.” London, IWA Publishing, 2000.
  • [3] Jenkins D., Richard M., Daigger G. „Manual on the causes and control of activated sludge bulking, foaming and other solids separation problems.” London, IWA Publishing, CRC Press, 2004.
  • [4] Seviour, R.J. “The current taxonomic status of the filamentous bacteria found in activated sludge plants.” In: “Microbial ecology of activated sludge” (Seviour R.J., Nielsen P.H., Eds.), pp. 169-190. London, IWA Publishing, 2009.
  • [5] Kragelund C., Levantesi C., Borger A., Thelen K., Eikelboom D., Thandoi V., Kong Y., van der Waarde J., Krooneman J., Rossetti J., Thomsen T.R., Nielsen P.R. “Identity, abundance and ecophysiology of filamentous Chloroflexi species present in activated sludge treatment plants.” FEMS Microbiology Ecology 59 (2007a): 671-682.
  • [6] Kragelund C., Thomsen T.R., Mielczarek A.T., Nielsen P.H. “Eikelboom’s morphotype 0803 in activated sludge belongs to the genus Caldilinea in the phylum Chloroflexi.” FEMS Microbiology Ecology 76 (2011): 451-462.
  • [7] Miura, Y., Watanabe, Y., Okabe, S. “Significance of Chloroflexi in performance of submerged membrane bioreactors (MBR) treating municipal wastewater.” Environmental Science and Technology 41 (22) (2007): 7787 - 7794.
  • [8] Blackall L.L., Parlett J.H., Hayward A.C., Minnikin D.E., Greenfield P.F. and Harbers A.E. “Nocardia pinensis sp.nov. an actinomycete found in activated sludge foams in Australia.” J. Gen. Microbiol. 135 (1989): 1547–1558.
  • [9] Soddell J.A., Seviour R.J., Blackall L.L., Hugenholtz P. “New foam-forming nocardioforms found in activated sludge.” Water Sci. Technol. 37 (1998): 495–502.
  • [10] Kragelund, C., Remesova, Z., Nielsen, J. L., Thomsen, T. R., Eales, K., Seviour, R., Wanner, J., Nielsen P.H. “Ecophysiology of mycolic acid-containing Actinobacteria (Mycolata) in activated sludge foams.” FEMS Microbiology Ecology, 61(1) (2007b): 174–84.
  • [11] Fiałkowska E., Fyda J., Pajdak-Stoś A., Wiąckowski K. “Osad czynny: biologia i analiza mikroskopowa.” Poland, Seidel i Przywecki, 2010.
  • [12] Nielsen P.H., Daims H., Lemmer H. (eds.) “FISH Handbook for Biological Wastewater Treatment. London”. IWA Publishing, 2009a.
  • [13] Nielsen P.H., Kragelund C., Seviour R.J., Nielsen J.L. “Identity and ecophysiology of filamentous bacteria in activated sludge.” FEMS Microbiol Rev. 33(2009b): 969-998.
  • [14] Mielczarek, A. T., Kragelund, C., Eriksen, P. S., & Nielsen, P. H. “Population dynamics of filamentous bacteria in Danish wastewater treatment plants with nutrient removal.” Water research, 46(12) (2012): 3781–3795.
  • [15] Loy, A., Horn, M., Wagner, M.: “ProbeBase: an online resource for rRNA-targeted oligonucleotide probes.” Nucleic Acids Res., 31(2003): 514–516.
  • [16] Muszyński, A., Łebkowska, M., Tabernacka, A., Miłobędzka, A. “From macro to lab-scale: Changes in bacterial community led to deterioration of EBPR in lab reactor.” Central European Journal of Biology, 8(2) (2012): 130–142.
  • [17] Collins T.J.: “ImageJ for microscopy.” Biotechniques, 43 (2007): 25–30.
  • [18] Eikelboom D.H. “Identification and Control of Filamentous Microorganisms in Industrial Wastewater Treatment Plants.” London, IWA Publishing, 2006.
  • [19] Eikelboom D., Geurkink B. “Filamentous microorganisms observed in industrial activated sludge plants.” Water Science and Technology 46(1-2) (2002): 535 - 542.
  • [20] Miłobędzka, A., Muszyński, A., “Filamentous Bacteria in Polish Full-scale Wastewater Treatment Plants with Nutrients Removal”. In: Proceedings of the IWA 6th Eastern European Young Water Professionals Conference ‘East Meets West’, Istanbul (2014): 903-912.
  • [21] Wanner J., Kragelund C., Nielsen P. H. “Microbiology of bulking.” In: “Microbial ecology of activated sludge” (Seviour R.J., Nielsen P.H., Eds.). London IWA Publishing, 2009, p 191-214.
  • [22] Nielsen, P. H., Kragelund, C., Nielsen, J. L., Tiro, S., Lebek, M., Rosenwinkel, K.-H., Gessesse, A. “Control of Microthrix parvicella in activated sludge plants by dosage of polyaluminium salts: possible mechanisms.” Acta Hydro- chim. Hydrobiol. 33 (2005): 255-261.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-b1d08eed-853a-4f3b-827e-1002cb53f94d
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