Wpływ produktów utlenienia lipidów na właściwości oraz proces elektroporacji dwuwarstwowych płaskich błon lipidowych

• Autorzy: Misiak H. , Kubica K.

Warianty tytułu

EN

Influence of the lipid peroxidation products on properties and electroporation process of the planar bilayer lipid membranes

• Języki publikacji: PL

Abstrakty

PL

Alternatywnym sposobem zwiększenia biernej przepuszczalności błon biologicznych dla cząsteczek biologicznie aktywnych, w tym leków, jest elektroporacja. Pod wpływem pola elektrycznego o odpowiednich parametrach w fazie lipidowej błon biologicznych pojawiają się pory. Podatność błony na elektroporację zależy od wielu czynników, w tym otoczenia komórki oraz od stanu lipidów tworzących błony. Wyniki badań doświadczalnych oraz teoretycznych dotyczące wpływu wybranych produktów utlenienia lipidów na podatność błony na elektroporację nie prowadzą do jednoznacznych konkluzji. Celem niniejszej pracy jest zbadanie wpływu wszystkich produktów utlenienia lecytyny jajecznej obecnych w utlenionej lecytynie na przebieg elektroporacji. Produkty utlenienia lecytyny zbadano techniką spektroskopii podczerwieni. Pomiary wartości parametrów elektrycznych i elektroporacyjnych utworzonych błon przeprowadzono za pomocą potencjostatu-galwanostatu w układzie 4-elektrodowym. Porównanie wyników badań z użyciem błon utworzonych z lecytyny utlenionej oraz nieutlenionej wykazało spadek wartości pojemności właściwej, wzrost rezystancji oraz stabilności błony zawierającej produkty utlenionej lecytyny. Uzyskane wyniki badań mogą być przydatne w optymalizacji protokołów elektroporacyjnych u pacjentów szczególnie narażonych na czynniki utleniające np. większą ekspozycję na UV.

EN

An alternative way of increasing the passive permeability of biological membranes to biologically active molecules, including drugs, is electroporation. Electric field of the relevant parameters applied to biological membranes creates pores in lipid part of membranes. Membrane susceptibility to electroporation depends on many factors: external environment of the cell, as well as a condition of membrane lipids. The results of experimental and theoretical research on influence of selected products of lipid oxidation on membrane susceptibility to electroporation do not lead to clear conclusions. The aim of this study is to examine the impact of all the products of oxidation of egg lecithin on the electroporation process. The oxidation of lecithin was examined by infrared spectroscopy. Measurements of the electrical membrane characteristics and parameters of electroporation were performed using a potentiostat-galvanostat in a four-electrode system. Comparison of the results obtained in experiments performed on oxidized and unoxidized lecithin have revealed a decline in the value of the specific capacity, an increase in the resistance, and stability of the membrane containing the oxidized lipids. These results may be useful in optimizing protocols of electroporation for patients, particularly those exposed to oxidizing agents, e.g. exposure to UV.

Słowa kluczowe

PL

elektroporacja; utlenianie lipidów; parametry elektroporacyjne

EN

electroporation; lipid oxidation; parameters of electroporation

• Wydawca: Jacek Doskocz
• Czasopismo: Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna
• Rocznik: 2017
• Tom: Vol. 23, nr 4
• Strony: 229--245
• Opis fizyczny: Bibliogr. 111 poz.

Twórcy

autor

Misiak H.

• Politechnika Wrocławska, Wydział Podstawowych Problemów Techniki, Katedra Inżynierii Biomedycznej, 50-370 Wrocław, Wybrzeże Wyspiańskiego 27

autor

Kubica K.

• Politechnika Wrocławska, Wydział Podstawowych Problemów Techniki, Katedra Inżynierii Biomedycznej, 50-370 Wrocław, Wybrzeże Wyspiańskiego 27

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