Pomiary profilu czasowego nagromadzenia fotouczulacza w komórkach rakowych jako narzędzie do optymalizacji terapii fotodynamicznej

• Autorzy: Bednarkiewicz A. , Gacek P. , Jellonek K. , Osiecka B. , Sadowski P. , Ziółkowski P.
• Konferencja: Inżynieria Biomedyczna i Telemedycyna; Sympozjum( 7.09.2007; Warszawa, Polska)
• Języki publikacji: PL

Abstrakty

EN

Monitoring of relevant parameters during therapy (PDT), further correlating with the treatment outcomes is highly necessary to deliver an effective and reproducible treatment modality. As far as ALA-PDT is concerned the timing of the contrast factor conditions the right moment for irradiations. We aim to deliver a portable system, based on surface fluorescence detection, for non invasive determination of issue pPIX level after topical administration of ALA. This method is better adapted then other absolute measurements of the tissue pPIX concentration. The tumor surface fluorescence intensity correlates with the tumor pPIX concetration. This method has been used to measure the fluorescence intensity form ALA- induced pPIX in lesions and adjacent normal skin. The mesaurements were carried out for 3-4 hours during PDT. We describe the results after using our system, guidelines for ALA-PDT timing and time-profile of pPIX tissue concentration.

Słowa kluczowe

PL

terapia fotodynamiczna; fluoroscencja; fotouczulacze

EN

photodynamic therapy

• Wydawca: Oficyna Wydawnicza Politechniki Warszawskiej
• Czasopismo: Prace Naukowe Politechniki Warszawskiej. Elektronika
• Rocznik: 2007
• Tom: z. 161
• Strony: 37--42
• Opis fizyczny: Bibliogr. 8 poz., wykr., schem.

Twórcy

autor

Bednarkiewicz A.

autor

Gacek P.

autor

Jellonek K.

autor

Osiecka B.

autor

Sadowski P.

autor

Ziółkowski P.

• Instytut Niskich Temperatur i Badań Strukturalnych PAN Wrocław,

Bibliografia

• [1] Nicolas Fotinos, 5-Aminolevulinic Acid Derivatives in Photomedicine: Characteristic, Application and Perspectives. Photochemistry and Photobiology, 2006, 82: 994-1015
• [2] A. Orenstein, The kinetics of protoporfiryn fluorescence during ALA-PDT in human malignant skin tumors, Cancer Letters (1997) 120: 229-234
• [3] A. L. Golub, The monitoring of ALA-induced Protoporphyrin IX Accumulation and Clearance in Patients with Skin Lesions by in Vivo Surface-Detected Fluorescence Spectroscopy, Lasers Med Sci (1999) 14: 112-122
• [4] M. B. Ericson, A spectroscopic study of the photobleaching of protoporphyrin IX in solution, Lasers Med Sci (2003) 18: 56-62
• [5] W.F. Cheong, S.A. Prahl, A. J. Welch, "A Review of the Optical Properties of Biological Tissues", IEEE J. Quantum Electronics 26, 1990, 2166-2185
• [6] Kennedy, J. C. and R. H. Pottier, Endogenous protoporphyrin IX, a clinically useful photosensitizer for photodynamic therapy J. Photochem. Photobiol. B (1992) 14: 275-292
• [7] A. Bednarkiewicz, P. Gacek, K. Jakubczyk, K. Jellonek, W. Stręk, "Protoporphyrin IX accumulation kinetics monitoring: Preliminary results", Acta Bio-Optica Et Informatica Medica, nr 1-2/2004 vol. 10, pg 43
• [8] Michail A. Ilias, Johan Richter, Evaluation of a Fiber-Optic Fluorescence Spectroscopy System to Assist Neurosurgical Tumor Resections, European Conference on Biomedical Optics 2007, Munich, Germany, SPIE-OSA Vol. 6631 66310W