The analysis of chromatin condensation state and transcriptional activity using DNA microarrays

• Autorzy: Widłak P. , Fujarewicz K.
• Języki publikacji: EN

Abstrakty

EN

The DNA microarray-based technique has been developed to semi-quantitatively measure the in vivo global chromatin condensation state at the resolution of a gene. Chromatin was fractionated due to the differential solubility of histone H1-containing and histone H1-free nucleosomes. A set of genes non-randomly distributed between histone H1-free (uncondensed or open) and histone H1-containing (condensed or closed) chromatin fractions has been identified. The transcript levels have been measured for the same group of genes. The correlation between transcriptional activity and chromatin fraction distribution of particular genes has been established.

Słowa kluczowe

EN

DNA microarrays; chromatin; transcription

PL

mikromacierz DNA; chromatyny; transkrypcja

• Wydawca: University of Silesia, Institute of Informatics, Computer Systems Department
• Czasopismo: Journal of Medical Informatics & Technologies
• Rocznik: 2003
• Tom: Vol. 6
• Strony: IP13--19
• Opis fizyczny: Bibliogr. 5 poz., rys.

Twórcy

autor

Widłak P.

• Department of Experimental and Clinical Radiobiology, Center of Oncology, Gliwice, Poland

autor

Fujarewicz K.

• Department of Automatic Control, Institute of Automation, Silesian Technical University, Gliwice, Poland

Bibliografia

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• [2] CHURCHILL GA., Fundamentals of experimental design for cDNA microarrays. Nature Genetics, supplement, vol. 32, pp. 490-495, 2002.
• [3] GARRARD WT., Histone H1 and the conformation of transcriptionally active chromatin. Bioessays, vol. 13, pp. 87-88, 1991.
• [4] HUANG SY., GARRARD WT., Electrophoretic analyses of nucleosomes and other protein-DNA complexes. Methods in Enzymology, vol. 170, pp. 116-142, 1989.
• [5] POLLACK JR., Parallel analysis of gene copy number and expression using cDNA microarrays. Methods in Molecular Biology, vol. 224, pp. 89-97, 2003.