Application of Mucor lipases for synthesis of saccharide esters

• Autorzy: Antczak T. , Szczęsna-Antczak M. , Patura J. , Hiler D. , Bielecki S.

Warianty tytułu

PL

Zastosowanie lipaz Mucor do syntezy estrów sacharydów

• Języki publikacji: EN

Abstrakty

EN

Produced in micro technical scale preparations of mycelium-bound Mucor circinelloides and M. racemosus lipases were used for synthesis of saccharide esters. The immobilized in situ Mucor lipases were additionally entrapped in a composite carrier composed of a mixture of poly(vinyl alcohol), gelatin, sodium alginate and chitozan, which was cross-linked with glutardialdehyde and polyphosphate. pH of lipase micro-environment was regulated in a range from 4 to 8 using buffer solutions. Synthesis of esters of glucose or sucrose and fatty acids was carried out at 50°C in a microreactor equipped with a stirrer and the water activity digital sensor. Either di-n-pentyl ether or its mixtures with petroleum ether were applied as reaction media. It was found that the natural antioxidant, carotene, activated these lipases in organic solvents what could be beneficial for the synthesis of esters of polyunsaturated fatty acids.

PL

Otrzymywane w skali mikrotechnicznej związane z mycelium lipazy Mucor circinelloides i Mucor racemosus zastosowano do syntezy estrów sacharydów. Immobilizowane in situ lipazy dodatkowo unieruchamiano w nośniku kompozytowym będącym mieszaniną PVA, żelatyny, alginianu sodu i chitozanu usieciowanego aldehydem glutarowym i polifosforanem. pH mikrootoczenia lipazy regulowano za pomocą buforu w zakresie pH 4-8. Syntezy estrów glukozy i sacharozy oraz kwasów tłuszczowych prowadzono w temp. 50°C w mikroreaktorze wyposażonym w mieszadło i czujnik aktywności wody. Jako środowisko reakcji stosowano eter di-n-pentylowy oraz jego mieszaniny z eterem naftowym. Stwierdzono, że naturalny antyoksydant karoten aktywuje stosowane lipazy w środowisku organicznym, co może być korzystne podczas syntezy estrów wielonienasyconych kwasów tłuszczowych.

Słowa kluczowe

EN

Mucor lipase; synthesis of saccharide esters; synthesis of fatty acids; microreactor

PL

lipaza Mucor; synteza estrów sacharydów; synteza kwasów tłuszczowych; mikroreaktor

• Wydawca: Wydawnictwo Politechniki Łódzkiej
• Czasopismo: Zeszyty Naukowe. Chemia Spożywcza i Biotechnologia / Politechnika Łódzka
• Rocznik: 2005
• Tom: Z. 69
• Strony: 23--37
• Opis fizyczny: Bibliogr. 31 poz.

Twórcy

autor

Antczak T.

• Technical University of Łódź, Faculty of Biotechnology and Food Sciences, Institute of Technical Biochemistry

autor

Szczęsna-Antczak M.

• Technical University of Łódź, Faculty of Biotechnology and Food Sciences, Institute of Technical Biochemistry

autor

Patura J.

• Technical University of Łódź, Faculty of Biotechnology and Food Sciences, Institute of Technical Biochemistry

autor

Hiler D.

• Technical University of Łódź, Faculty of Biotechnology and Food Sciences, Institute of Technical Biochemistry

autor

Bielecki S.

• Technical University of Łódź, Faculty of Biotechnology and Food Sciences, Institute of Technical Biochemistry

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