Studies on interaction between (m-AMSA) and DNA by viscometric and melting profiles methods

• Autorzy: Leszczyński T. , Duński H.

Warianty tytułu

PL

Badania oddziaływań pomiędzy m-AMSA a DNA metodami wiskozymetrycznymi oraz profili topnienia DNA

• Języki publikacji: EN

Abstrakty

EN

We have examined the interactions between m-AMSA and CT-DNA, using melting profiles and viscometric techniques methods. We observed the increase of thermal stability of DNA after binding m-AMSA, depending on the concentration of the ligand in the solution. The maximal increase of the melting temperature of the m-AMSA/DNA system in comparison to pure DNA, was 6 o C for the concentration of m-AMSA to DNA of 0,5. Viscometric examination of interaction of m-AMSA with DNA unambiguously indicate intercalation type of bounds. In the saturation state, 2,76 base pairs occur for one ligand molecule.

PL

W pracy badano oddziaływania pomiędzy m-AMSA i CT-DNA metodami wiskozymetrycznymi oraz profili topnienia DNA. Zaobserwowano wzrost stabilnoci termicznej DNA po związaniu m-AMSA, która zależała od stosunku stężeń m-AMSA/DNA. Największy wzrost temperatury topnienia badanego układu względem roztworu DNA wyniósł 6°C dla stosunku m-AMSA/DNA = 0,5. Pomiary wiskozymetryczne wskazały jednoznacznie na interkalacyjny sposób wiązania m-AMSA z DNA. W stanie wysycenia jedna cząsteczka m-AMSA przypadała średnio na 2,76 par zasad DNA.

Słowa kluczowe

EN

m-amsa; DNA; viscometry; melting profiles

PL

m-amsa; DNA; wiskozymetria; profile topnienia dna

• Wydawca: Wydawnictwo Politechniki Łódzkiej
• Czasopismo: Zeszyty Naukowe. Chemia Spożywcza i Biotechnologia / Politechnika Łódzka
• Rocznik: 2006
• Tom: Z. 70
• Strony: 13--22
• Opis fizyczny: Bibliogr. 27 poz.

Twórcy

autor

Leszczyński T.

autor

Duński H.

• Wydział Biotechnologii i Nauk o Żywności, Politechnika Łódzka

Bibliografia

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