Mikroprzepływowe immunoczujniki z detekcją amperometryczną

• Autorzy: Baraniecka A. , Górska M. , Zaborowski M. , Prokaryn P. , Szczypiński R. , Skwara K. , Szmigiel D. , Sierakowski A. , Nieprzecki M.

Warianty tytułu

EN

Microfluidic immunosensors with amperometric detection

• Języki publikacji: PL

Abstrakty

PL

Artykuł obejmuje przekrój zagadnień związanych z mikroprzepływowymi amperometrycznymi immunoczujnikami. Wymienione zostały przykładowe technologie i materiały konstrukcyjne używane do budowy mikroprzepływowych modułów i elektrod oraz immunoenzymatyczne metody analityczne stosowane w immunoczujnikach, takie jak ELISA i ELISPOT. Zostały również przedstawione zagadnienia związane z przepływem w systemach mikroprzepływowych. Opisano technologię matryc z SU-8, struktur mikroprzepływowych wykonanych z PDMS-u oraz laminowanych elektrod Au/Ti na podłożu polimerowym. Uzyskano szczelne mikroprzepływowe układy ze złotymi elektrodami na podłożu z PDMS. Dzięki zastosowaniu trawienia jonowego podłoża przed napyleniem warstw metali oraz zastosowaniu pośredniej warstwy tytanowej, elektrody wykazują dobrą adhezję do podłoża. Dodatkowo została zastosowana warstwa PDMS-u, chroniąca ścieżki metalizacji przed pękaniem, w której plazmowo wytworzono okienka kontaktów elektrycznych i elektrod aktywnych elektrochemicznie. Elektrody charakteryzowały się małą rezystancją elektryczną, choć nie uzyskano zadawalającej powtarzalności ich wykonania. Struktury mikroprzepływowe wraz z elektrodami mogą być zastosowane w amperometrycznym immunoczujniku do pomiaru różnych antygenów, w zależności od użytych immunoreagentów m.in. do pomiaru stężenia fibrynogenu we krwi, w celu określenia ryzyka wystąpienia udaru niedokrwiennego mózgu oraz chorób układu krążenia.

EN

This paper describes some problems related with microfluidic amperometric immunosensors. It contains brief review of technology and constructive materials for microfluidic systems and electrodes. In addition, immunoenzymatic analytical methods like: ELISA and ELISPOT as well as some flow phenomena in microfluidic environment are presented. Polymeric SU-8 masters, PDMS-based microfluidic structures and laminated Au/Ti electrodes on polymeric substrates were fabricated. The microfluidic structures were successfully bonded after oxygen plasma surface activation. Thanks to applying reactive ion etching prior Au sputtering and Ti adhesive layer deposition, the Au/Ti electrodes exhibited a very good adhesion. After patterning, the electrodes were protected by a thin PDMS layer. Openings for electrodes and electrical contact pads were etched by (SF₆ + O₂) plasma. The electrodes had a good electrical conductivity but rather poor reproducibility. The microfluidic structures can be applied in amperometric immunosensor to measure concentration of different antigens e.g. concentration of fibrinogen in blood for evaluation of brain stroke and cardiovascular diseases risk.

Słowa kluczowe

PL

immunoczujnik; amperometria; mikrokanały; PDMS; monoklonalne przeciwciała; test ELISA; test ELISPOT

EN

immunosensor; amperometry; microchannels; PDMS; monoclonal antibodies; ELISA test; ELISPOT test

• Wydawca: Wydawnictwo SIGMA-NOT
• Czasopismo: Elektronika : konstrukcje, technologie, zastosowania
• Rocznik: 2009
• Tom: Vol. 50, nr 12
• Strony: 100--106
• Opis fizyczny: Bibliogr. 67 poz., il., rys.

Twórcy

autor

Baraniecka A.

autor

Górska M.

autor

Zaborowski M.

autor

Prokaryn P.

autor

Szczypiński R.

autor

Skwara K.

autor

Szmigiel D.

autor

Sierakowski A.

autor

Nieprzecki M.

• Instytut Technologii Elektronowej, Warszawa

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