Zastosowanie technik immunoenzymatycznych i mikrofluidycznych do amperometrycznego oznaczania stężenia białka C-reaktywnego

• Autorzy: Baraniecka A. , Kazimierczak B. , Kruk J. , Pijanowska D. G. , Torbicz W.

Warianty tytułu

EN

Application of immunoenzymatic and microfluidic techniques for amperometric determination of C-reactive protein concentration

• Języki publikacji: PL

Abstrakty

PL

CRP jest białkiem ostrej fazy i jednym z najlepszych niespecyficznych markerów określającym ryzyko oraz zaawansowanie choroby niedokrwiennej serca. Bardzo przydatne stają się w tym przypadku wielofunkcyjne urządzenia diagnostyczne z szybką amperometryczną detekcją oraz funkcją wstępnego rozdziału próbki. Układy mikroprzepływowe umożliwiają taki rozdział przy małej objętości badanych próbek i reagentów, co znacząco obniża koszty testu. Z wyjątkiem nowych aptaczujników, jedynie immunoczujniki zapewniają specyficzność wobec poszukiwanego analitu w próbce. W nieprzezroczystych próbkach, jakimi są płyny fizjologiczne, niemożliwe jest zastosowanie optycznych metod detekcji, stąd konieczne jest zastosowanie metody znakowania enzymem przeciwciał w immunoczujnikach i użycie innego rodzaju detekcji np. elektrochemicznej. Ze względu na niski poziom szumów tła i możliwość szybkiej detekcji, najczęściej stosuje się detekcję amperometryczną. Celem naszych prac jest opracowanie amperometrycznych immunoczujników mikroprzepływowych do diagnostyki medycznej do oznaczania CRP.

EN

CRP is acute phase protein and one of the best nonspecific diagnostic markers in heart diseases. Microfluidic systems offer function of preliminary probe separation with small consumption of probes and reagents, which decrease cost of unit test. Except newly developed aptasensors, only immunosensors provide specificity to determined analit in composed analyzed probe. In the case of nontransparent samples, like physiological fluids, immunosensors with electrochemical detection are sensors of choice, e.g. amperometric. For electrochemical detection enzyme labeled antibody is used. In order to decrease background noise and obtain fast response, amperometric sensors are mostly applied. An aim of our research is development of microfluidic type amperometric immunosensors for medical diagnostics, in particular for CRP determination.

Słowa kluczowe

PL

białko C-reaktywne; immunoczujnik; układ mikro-fluidyczny; detekcja amperometryczna; test ELISA

EN

C-reactive protein; immunosensor; microfluidic system; amperometric detection; ELISA test

• Wydawca: Wydawnictwo SIGMA-NOT
• Czasopismo: Elektronika : konstrukcje, technologie, zastosowania
• Rocznik: 2009
• Tom: Vol. 50, nr 11
• Strony: 79--83
• Opis fizyczny: Bibliogr. 61 poz., wykr.

Twórcy

autor

Baraniecka A.

autor

Kazimierczak B.

autor

Kruk J.

autor

Pijanowska D. G.

autor

Torbicz W.

• Instytut Technologii Elektronowej, Warszawa

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