Enzymatyczne bioczujniki oparte na dehydrogenazach wykorzystujących NAD⁺

• Autorzy: Kossakowska A. , Pijanowska D. , Torbicz W.

Warianty tytułu

EN

Enzymatic biosensors based on NAD⁺ : dependent dehydrogenases

• Języki publikacji: PL

Abstrakty

PL

Zaprezentowano przykłady oraz omówiono zastosowanie bioczujników enzymatycznych opartych na dehydrogenazach, wykorzystujących koenzym NAD+. Opisano różne technologie wytwarzania bioczujników oraz różne metody detekcji. Porównano dwa sposoby regeneracji koenzymu za pomocą dodatkowego systemu enzymatycznego przy wykorzystaniu diaforazy oraz mediatora przenoszenia elektronów. Przedstawiono jedną z metod ulepszania bioczujników amperometrycznych, polegającą na zastosowaniu elektroprzewodzącego polipirolu domieszkowanego elektroaktywnym mediatorem (żelazocyjanek).

EN

This paper presents examples and application of enzymatic biosensors based on NAD+ -dependent dehydrogenases. Different technologies of biosensors fabrication and different methods of detection are described. Two methods of the coenzyme regeneration with additional enzymatic system using diaphorase and with the electron transfer mediator are compared. A method of improving of amperometric biosensors by applying electroconducting polypyrrole doped with electroactive mediator (ferrocyanide) is presented.

Słowa kluczowe

PL

bioczujniki; dehydrogenazy; regeneracja koenzymu; immobilizacja; polimery przewodzące

EN

biosensors; dehydrogenases; coenzyme regeneration; mediators; immobilization; conducting polymers

• Wydawca: Wydawnictwo SIGMA-NOT
• Czasopismo: Elektronika : konstrukcje, technologie, zastosowania
• Rocznik: 2008
• Tom: Vol. 49, nr 5
• Strony: 113--118
• Opis fizyczny: Bibliogr. 33 poz., rys., wykr.

Twórcy

autor

Kossakowska A.

autor

Pijanowska D.

autor

Torbicz W.

• Instytut Biocybernetyki i Inżynierii Biomedycznej PAN, Warszawa

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