Porównanie technik spektroskopii FTIR stosowanych do identyfikacji materiału biologicznego

• Autorzy: Mularczyk-Oliwa M. , Kwaśny M. , Włodarski M. , Kaliszewski M.

Warianty tytułu

EN

Comparison of FTIR spectroscopy techniques used for biological material identification

• Języki publikacji: PL

Abstrakty

PL

Spektroskopia podczerwieni FTIR była wielokrotnie demonstrowana przez liczne zespoły badawcze jako jedna z głównych metod identyfikacji i klasyfikacji materiału biologicznego, jakim są żywe bakterie oraz ich spory. W niniejszej pracy porównano techniki, którymi dysponuje spektroskopia FTIR: klasyczną technikę transmisyjną, technikę wielokrotnego wewnętrznego odbicia - Horizontal Attenuated Total Reflectance (HATR) oraz technikę dyfuzyjnego rozproszenia - Diffuse Reflectance Infrared Fourier Transform (DRIFT). Powyższe techniki zastosowano do analizy materiału biologicznego: bakterii i ich sporów, podłoża dla bakterii, pyłków, aminokwasów i białek. Stwierdzono powtarzalność wyników badań tej samej substancji wykonanych trzema technikami. Techniki HATR i DRIFT mogą stanowić alternatywę do tradycyjnej techniki transmisyjnej ze względu na możliwość bezpośredniego pomiaru materiału biologicznego bez wcześniejszej obróbki. Na podstawie analizy widm uzyskanych za pomocą HATR i DRIFT popartej dodatkowo analizą statystyczną - analizą głównych składowych (PCA), stwierdzono przydatność technik w rozróżnianiu żywych bakterii od ich form przetrwalnikowych oraz podłoża.

EN

Abstract. as spores and vegetative cells has been demonstrated by several laboratories using FTIR spectroscopy. In the present work three techniques of FTIR spectroscopy have been discussed: classical transmission techniques using transmission mode, Diffuse Reflectance Infrared Fourier Transform (DRIFT), and Horizontal Attenuated Total Reflectance (HATR). Different groups of biological material have been measured: vegetative cells of bacteria, bacterial spores, background materials, pollens, aminoacids, and proteins. Spectra of the same material achieved using three different FTIR techniques were similar to each other. Main advantage of HATR and DRIFT in comparison with a transmission mode is minimum sample preparation of biological material. These results also demonstrate that FTIR techniques in combination with statistical Principal Component Analysis (PCA) provide rapid and reliable distinction between vegetative bacteria, bacterial spores, and background particles.

Słowa kluczowe

PL

spektroskopia FTIR; HATR; DRIFT; bakteria żywa; spory

EN

FTIR; HATR; DRIFT; vegetative bacteria; spores

• Wydawca: Wojskowa Akademia Techniczna im. Jarosława Dąbrowskiego
• Czasopismo: Biuletyn Wojskowej Akademii Technicznej
• Rocznik: 2009
• Tom: Vol. 58, nr 3
• Strony: 239--257
• Opis fizyczny: Bibliogr. 41 poz., tab., wykr.

Twórcy

autor

Mularczyk-Oliwa M.

autor

Kwaśny M.

autor

Włodarski M.

autor

Kaliszewski M.

• Wojskowa Akademia Techniczna, Instytut Optoelektroniki, 00-908 Warszawa, ul. S. Kaliskiego 2

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