Low cost lasers challenge ultrafast systems in two-photon excitation applications

• Autorzy: Tirri M. , Vaarno J. , Soini J. , Hanninen P.
• Języki publikacji: EN

Abstrakty

EN

Although excellent research tools, the ultrafast laser systems are poorly suited for routine laboratory instrumentation, simply due to their high price. Our research group has shown tat application of two-photon excitation need not to be limited to those laboratories that can afford the price and maintenance of ultrafast laser systems. With certain compromises and well-designed experiments, low-cost lasers can be used in utilizing the positive properties of two-photon excitation. The purpose of this publication is to show examples of bioanalytical applications that are possible using low-cost lasers. A bioaffinity assay using microparticles as bioactive carriers and assay for characterization of cell surface antigens are presented. By direct comparison of the example assays in two instrumental set-ups, a quantity is given to the 'compromises' that are made by replacing an ultrafast system with low cost equipment.

Słowa kluczowe

EN

two-photon excitation; bioanalytics; bioaffinity assays; in-vitro diagnostics

• Wydawca: Stowarzyszenie Elektryków Polskich ; Polska Akademia Nauk ; Wojskowa Akademia Techniczna im. Jarosława Dąbrowskiego
• Czasopismo: Opto-Electronics Review
• Rocznik: 2003
• Tom: Vol. 11, No. 1
• Strony: 39--44
• Opis fizyczny: Bibliogr. 15 poz.

Twórcy

autor

Tirri M.

• Laboratory of Biophysics, Institute of Biomedicine, University of Turku Po Box 123, FIN-20521 Turku, Finland

autor

Vaarno J.

• Laboratory of Biophysics, Institute of Biomedicine, University of Turku Po Box 123, FIN-20521 Turku, Finland

autor

Soini J.

• Laboratory of Biophysics, Institute of Biomedicine, University of Turku Po Box 123, FIN-20521 Turku, Finland

autor

Hanninen P.

• Laboratory of Biophysics, Institute of Biomedicine, University of Turku Po Box 123, FIN-20521 Turku, Finland

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