Cadmium and copper toxicity assessment in activated sludge using TTC bioassay

• Autorzy: Małachowska-Jutsz A. , Matyja K. , Ziembińska A.

Warianty tytułu

PL

Wykorzystanie testu TTC do oceny toksyczności kadmu i miedzi w osadzie czynnym

• Języki publikacji: EN

Abstrakty

EN

The aim of this work was to determine the effect of various cadmium and copper concentrations on the activated sludge dehydrogenase activity. The investigations were carried out in six aerated chambers with activated sludge, volume of 1L each, by the continuous culture method (one control chamber, not contaminated with heavy metals and five with 0.5; 1; 2; 4; 8 mg L-1 Cu+2 and 0.1; 0.3; 0.9; 2.7; 8.1 mg L-1 Cd2+). Cadmium sulfate and copper sulfate as a source of heavy metals were used. The concentrations of these metal ions, causing 50% dehydrogenase activity inhibition were determined. The particular attention was paid to the toxic effect of metal ions, as well as the variations of the microbial respiration activity proceeded during toxins exposition. The investigation showed that even the lowest concentration of the investigated metal ions caused significant changes of the activated sludge dehydrogenases activity. Copper ions showed to be more toxic than cadmium ions.

PL

Celem niniejszej pracy było określenie wpływu różnych stężeń kadmu i miedzi na aktywność dehydrogenaz mikroorganizmów osadu czynnego. Badania przeprowadzono z wykorzystaniem sześciu komór z napowietrzanym osadem czynnym, każda o objętości 1L (niezanieczyszczona próba stanowiła kontrolę, a pozostałe zawierały: 0,5; 1; 2; 4; 8 mg L-1 Cu+2 oraz 0,1; 0,3; 0,9; 2,7; 8,1 mg L-1 Cd2+). Metale wprowadzano do zawiesiny w postaci siarczanu kadmu i siarczanu miedzi. Wyznaczono stężenia jonów powodujące 50% zahamowanie aktywności dehydrogenaz. Szczególną uwagę zwrócono na porównanie toksyczności jonów obu metali oraz na zachodzące w czasie pod ich wpływem zmiany aktywności oddechowej mikroorganizmów. Badania wykazały, że nawet najniższe z zastosowanych stężeń badanych związków powodowały istotne zmiany w aktywności dehydrogenaz osadu. Jony miedzi okazały się być bardziej toksyczne niż jony kadmu.

Słowa kluczowe

EN

cadmium; copper; heavy metal; toxicity; dehydrogenase; TTC

PL

miedź; kadm; metale ciężkie; toksyczność; dehydrogenaza; test TTC

• Wydawca: Institute of Environmental Engineering, Polish Academy of Sciences
• Czasopismo: Archives of Environmental Protection
• Rocznik: 2011
• Tom: Vol. 37, no. 4
• Strony: 85--94
• Opis fizyczny: Bibliogr. 33 poz., tab., wykr.

Twórcy

autor

Małachowska-Jutsz A.

autor

Matyja K.

autor

Ziembińska A.

• The Silesian University of Technology, Environmental Biotechnology Department, Akademicka 2, 44-100 Gliwice,

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