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Induction of stress proteins in the presence of cadmium in the Baltic blue mussel Mytilus trossulus [commun.]

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The exposure of organisms to environmental stressors affects the expression levels of certain 'stress proteins' that play an important role in protein homeostasis and stress tolerance. Examining the protein profiles by SDS-PAGE and Western blotting analysis, mussels Mytilus trossulus were exposed to cadmium, which induced a number of Hsp70 proteins in accordance with the metal concentrations. In immunodetection two commercial monoclonal antibodies were used to monitor this response in gill tissue. It follows that Hsp70, which is typically induced by moderate heat-shock treatment, is in most cases also induced in the presence of cadmium.
Czasopismo
Rocznik
Strony
153--156
Opis fizyczny
Bibliogr. 7 poz., wykr.
Twórcy
  • Institute of Oceanology, Polish Academy of Sciences, Sopot
Bibliografia
  • 1. Bradford M., 1976, A rapid and simple method for quantitation of microgram quantities of protein using the principle of dye binding, Anal. Biochem., 72, 248-254.
  • 2. Depledge M. H., 1994, The rational basis for the use of biomarkers as ecotoxicological tools, [in:] Non-destructive biomarkers in vertebrates, M. C. Fossi and C. Leonzio (eds.), Lewis Publ., Boca Raton, Florida, 271-295.
  • 3. Laemmli U. L., 1970, Cleavage of structure proteins during the assembly of bacteriofage T4, Nature, 277, 680-685.
  • 4. McCarthy J. F., Shugart L. R., (eds.), 1990, Biomarkers of environmental contamination, Lewis Publ., Boca Raton, Florida, 335 pp.
  • 5. Radłowska M., Pempkowiak J., 1996, Qualitative analysis of stress proteins induced by cadmium in the blue mussel Mytilus trossulus, Oceanol. Stud., 3, 133-137.
  • 6. Sanders B. M., Martin L. S., 1993, Stress proteins as biomarkers of contaminant exposure in archived environmental samples, Sci. Total Environm., 139/140, 459-470.
  • 7. Smerdon G. R., Chapple J. P., Hawkins J. S., 1995, The simultaneous immunological detection of four stress-70 protein isoforms in Mytilus edulis, Mar. Environm. Res., 40, 399-407.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BUS8-0015-0047
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