Variability of the specific fluorescence of chlorophyll in the ocean. Part 2. Fluorometric method of chlorophyll a determination

• Autorzy: Ostrowska M. , Matorin D.N. , Ficek D.
• Języki publikacji: EN

Abstrakty

EN

Two methods of determining the chlorophyll a concentration in the sea have been formulated on the basis of artificially induced fluorescence measured with the aid of submersible fluorometers. The method of statistical correlation is founded on the empirical relationship between fluorescence and chlorophyll concentration. The theoretical model of fluorescence described in Part 1 of this paper (see Ostrowska et al. 2000, this volume) provides the basis of the other method, the physical method. This describes the dependence of the specific fluorescence of phytoplankton on the chlorophyll concentration, a diversity of photophysiological properties of phytoplankton and the optical characteristics of the fluorometer. In order to assess their practicability, the methods were subjected to empirical verification. This showed that the physical method yielded chlorophyll concentrations of far greater accuracy. The respective error factors of the estimated chlorophyll concentration were x = 2.07 for the correlation method and x = 1.5 for the physical method. This means that the statistical logarithmic error varies from -52 to +107% in the case of the former method but only from -33 to +51% in the case of the latter. Thus, modifying the methodology has much improved the accuracy of chlorophyll determinations.

Słowa kluczowe

EN

plant luminescence; chlorophyll adetermination; fluorometric method

• Wydawca: Polish Academy of Sciences, Institute of Oceanology ; Elsevier
• Czasopismo: Oceanologia
• Rocznik: 2000
• Tom: No. 42 (2)
• Strony: 221--229
• Opis fizyczny: Bibliogr. 6 poz., tab., wykr.

Twórcy

autor

Ostrowska M.

• Institute of Oceanology, Polish Academy of Sciences

autor

Matorin D.N.

• Department of Biophysics, Faculty of Biology, Moscow State University

autor

Ficek D.

• Institute of Physics, Pedagogical University

• Institute of Oceanology, Polish Academy of Sciences, Powstańców Warszawy 55, 81-712 Sopot, Pola,

Bibliografia

• 1. Karabashev G. S., 1987, Fluorescence in the ocean, Gidrometeoizdat, Leningrad, 200 pp., (in Russian).
• 2. Kolber Z., Falkowski P. G., 1993, Use of active fluorescence to estimate phytoplankton photosynthesis ‘in situ’, Limnol. Oceanogr., 38 (8), 1646-1665.
• 3. Ostrowska M., 1990, Fluorescence ‘in situ’ method for the determination of chlorophyll a concentration in sea, Oceanologia, 29, 175-202.
• 4. Ostrowska M., Majchrowski R., Matorin D. N., Woźniak B., 2000, Variability of the specific fluorescence of chlorophyll in the ocean. Part 1. Theory of classical ‘in situ’ chlorophyll fluorometry, Oceanologia, 42 (2), 203-219.
• 5. Woźniak B., Dera J., Koblentz-Mishke O. I., 1992a, Bio-optical relationships for estimating primary production in the Ocean, Oceanologia, 33, 5-38.
• 6. Woźniak B., Dera J., Koblentz-Mishke O. I., 1992b, Modelling the relationship between primary production, optical properties, and nutrients in the sea, Ocean Optics 11, Proc. SPIE, 1750, 246-275