How do marine bacteria produce light, why are they luminescent, and can we employ bacterial bioluminescence in aquatic biotechnology?

• Autorzy: Węgrzyn G. , Czyż A.
• Języki publikacji: EN

Abstrakty

EN

Bioluminescence, the phenomenon of light production by living organisms, occurs in forms of life as various as bacteria, fungi and animals. Nevertheless, light-emitting bacteria are the most abundant and widespread of luminescent organisms. Interestingly, most species of such bacteria live in marine environments. In this article, the biochemical mechanism of bacterial luminescence and its genetic regulation are summarized. Although the biochemistry and genetics of light emission by cells have been investigated in detail, the biological role of bacterial luminescence has remained obscure. Here, we discuss recent discoveries that shed new light on this problem. Finally, we provide examples of how bacterial luminescence can be employed in marine biotechnology, especially in the detection of toxic and mutagenic pollution in aquatic environments.

Słowa kluczowe

EN

bioluminescence; luminescent bacteria; quorum sensing; DNA repair; detection of mutagenic pollution in marine environments

• Wydawca: Polish Academy of Sciences, Institute of Oceanology ; Elsevier
• Czasopismo: Oceanologia
• Rocznik: 2002
• Tom: No. 44 (3)
• Strony: 291--305
• Opis fizyczny: Bibliogr. 58 poz.

Twórcy

autor

Węgrzyn G.

• Department of Molecular Biology, University of Gdańsk, Kładki 24, PL-80-822 Gdańsk, Poland
• Institute of Oceanology, Polish Academy of Sciences, Św. Wojciecha 5, PL-81-347 Gdynia, Poland

autor

Czyż A.

• Laboratory of Molecular Biology (affiliated with the University of Gdańsk), Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Kładki 24, PL-80-822 Gdańsk, Poland

Bibliografia

• [1] Bar R., Ulitzur S., 1994, Bacterial toxicity of cyclodextrins: luminous Escherichia coli as a model, Appl. Microbiol. Biotechnol., 41, 574-577.
• [2] Bassler B. L., Silverman M. R., 1995, Intercellular communication in marine Vibrio species: density-dependent regulation of the expression of bioluminescence, [in:] Two-component signal transduction, J. A. Hoch & T. J. Silhavy (eds.), Amer. Soc. Microbiol., Washington D. C., 431-445.
• [3] Bassler B. L., Wright M., Silverman M. R., 1994, Sequence and function of luxO, a negative regulator of luminescence in Vibrio harveyi, Mol. Microbiol., 12, 403-412.
• [4] Baumann P., Baumann L., Reichelt J. L., 1973, Taxonomy of marine bacteria: Beneckea parahaemolytica and Beneckea alginolytica, J. Bacteriol., 113, 1144-1155.
• [5] Belas R., Mileham A., Cohn D., Hilmen M., Simon M., Silverman M., 1982, Bacterial luminescence: isolation and expression of the luciferase genes from Vibrio harveyi, Science, 218, 791-793.
• [6] Belkin S., Steiber M., Tiehm A., Frimmel F. H., Abeliovich A., Werner P., Ulitzur S., 1994, Toxicity and genotoxicity enhancement during polycyclic aromatic hydrocarbons biodegradation, Environ. Tox. Wat. Qual., 9, 303-309.
• [7] Ben-Israel O., Ben-Israel H., Ulitzur S., 1998, Identification and quantification of toxic chemicals by the use of Escherichia coli carrying lux genes fused to stress promoters, Appl. Environ. Microbiol., 64, 4346-52.
• [8] Ben-Itzhak J., Levi B. Z., Shor R., Lanir A., Bassan H. M., Ulitzur S., 1985, The formation of genotoxic metabolites of benzo[a]pyrene by isolated perfused rat liver, as detected by bioluminescent assay, Mutat. Res., 147, 107-112.
• [9] Brenner A., Belkin S., Ulitzur S., Abeliovich A., 1993a, Fast assessment of toxicant adsorption on activated carbon using a luminous bacteria bioassay, Wat. Sci. Technol., 27, 113-120.
• [10] Brenner A., Belkin S., Ulitzur S., Abeliovich A., 1993b, Evaluation of activated carbon adsorption capacity by a toxicity bioassay, Wat. Sci. Technol., 27, 1577-1583.
• [11] Brenner A., Belkin S., Ulitzur S., Abeliovich A., 1994, Utilization of a bioluminescence toxicity assay for optimal design of biological and physico-chemical wastewater treatment processes, Environ. Tox. Wat. Qual., 9, 311-316.
• [12] Bulich A. A., Isenberg D. L., 1981, Use of the bioluminescent bacterial system for the rapid assessment of aquatic toxicity, ISA Trans., 20, 29-33.
• [13] Chatterjee J., Miyamoto M., Meighen E. A., 1996, Autoregulation of luxR: the Vibrio harveyi lux-operon activator functions as a repressor, Mol. Microbiol., 20, 415-425.
• [14] Czyż A., Jasiecki J., Bogdan A., Szpilewska H., Węgrzyn G., 2000a, Genetically modified Vibrio harveyi strains as potential bioindicators of mutagenic pollution of marine environments, Appl. Environ. Microbiol., 66, 599-605.
• [15] Czyż A., Plata K., Węgrzyn G., 2002a, Induction of light emission by luminescent bacteria treated with UV light and chemical mutagens, J. Appl. Genet., 43, 377-389.
• [16] Czyż A., Szpilewska H., Dutkiewicz R., Kowalska W., Biniewska-Godlewska A., Węgrzyn G., 2002b, Comparison of the Ames test and newly developed assay for detection of mutagenic pollution of marine environments, Mutat. Res., 519, 67-74.
• [17] Czyż A., Węgrzyn G., 2001a, On the function and evolution of bacterial luminescence, [in:] Bioluminescence and chemiluminescence, J. F. Case, P. J. Herring, B. H. Robinson, S. H. D. Haddock, L. J. Kricka & P. E. Stanley (eds.), World Sci. Publ. Company, Singapore, 31-34.
• [18] Czyż A., Wróbel B., Węgrzyn G., 2000b, Vibrio harveyi bioluminescence plays a role in stimulation of DNA repair, Microbiology, 146, 283-288.
• [19] Czyż A., Zielke R., Konopa G., Węgrzyn G., 2001b, A Vibrio harveyi insertional mutant in the cgtA (obg, yhbZ) gene, whose homologues are present in diverse organisms ranging from bacteria to humans and are essential genes in many bacterial species, Microbiology, 147, 183-191.
• [20] Dutkiewicz R., Słomińska M., Węgrzyn G., Czyż A., 2002, Overexpression of the cgtA (yhbZ, obgE) gene coding for an essential GTP-binding protein impairs the regulation of chromosomal functions in Escherichia coli, Curr. Microbiol., (in press).
• [21] Fitzgerald J. M., 1977, Classification of luminous bacteria from the light organ of the Australian pinecone fish Cleidophus glorimaris, Arch. Microbiol., 112, 153-156.
• [22] Freeman J. A., Bassler B. L., 1999, A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi, Mol. Microbiol., 31, 665-677.
• [23] Freeman J. A., Lilley B. N., Bassler B. L., 2000, A genetic analysis of the functions of LuxN: a two-component hybrid sensor kinase that regulates quorum sensing in Vibrio harveyi, Mol. Microbiol., 35, 139-149.
• [24] Jabłoński E., DeLuca M., 1978, Studies of control of luminescence in Beneckea harveyi: properties of the NADH and NADPH:FMN oxidoreductases, Biochemistry, 17, 672-678.
• [25] Klein G., Walczak R., Krasnowska E., Błaszczak A., Lipińska B., 1995, Characterization of heat-shock response of the marine bacterium Vibrio harveyi, Mol. Microbiol., 16, 801-811.
• [26] Klein G., Żmijewski M., Krzewska J., Czeczatka M., Lipińska B., 1998, Cloning and characterization of the dnaK heat shock operon of the marine bacterium Vibrio harveyi, Mol. Gen. Genet., 259, 179-189.
• [27] Labas Y. A., Matz M. V., Zakhartchenko V. A., 2001, On the origin of bioluminescent systems, [in:] Bioluminescence and chemiluminescence, J. F. Case, P. J. Herring, B. H. Robinson, S. H. D. Haddock, L. J. Kricka & P. E. Stanley (eds.), World Sci. Publ. Company, Singapore, 91-94.
• [28] Lange J. H., Thomulka K. W., 1997, Use of the Vibrio harveyi toxicity test for evaluating mixture interactions of nitrobenzene and dinitrobenzene, Ecotoxicol. Environ. Safety, 38, 2-12.
• [29] Levi B. Z., Kuhn J. C., Ulitzur S., 1986, Determination of the activity of 16 hydrazine derivatives in the bioluminescence test for genotoxic agents, Mutat. Res., 173, 233-237.
• [30] Makemson J. C., 1986, Luciferase-dependent oxygen consumption by bioluminescent Vibrio, J. Bacteriol., 165, 461-466.
• [31] Meighen E. A., 1994, Genetics of bacterial bioluminescence, Ann. Rev. Genet., 28, 117-139.
• [32] Min J., Kim E. J., la Rossa R. A., Gu M. B., 1999, Distinct responses of a recA::luxCDABE Escherichia coli strain to direct and indirect DNA damaging agents, Mutat. Res., 442, 61-68.
• [33] Miyamoto C. M., Chatterjee J., Swartzman E., Szittner R., Meighen E. A., 1996, The role of lux autoinducer in regulating luminescence in Vibrio harveyi; control of luxR expression, Mol. Microbiol., 19, 767-775.
• [34] Miyamoto C. M., Lin Y. H., Meighen E. A., 2000, Control of bioluminescence in Vibrio fischeri by the LuxO signal response regulator, Mol. Microbiol., 36, 594-607.
• [35] Morin J. G., Harrington A., Nealson K., Krieger N., Baldwin T. O., Hastings J. W., 1975, Light for all reasons: versatility in the behavioral repertoire of the flashlight fish, Science, 190, 74-76.
• [36] Nealson K. H., 1978, Isolation, identification and manipulation of luminous bacteria, Meth. Enzymol., 57, 153-166.
• [37] Nealson K. H., Hastings J. W., 1979, Bacterial bioluminescence: its control and ecological significance, Microbiol. Rev., 43, 496-518.
• [38] Ptitsyn L. R., Horneck G., Komova O., Kozubek S., Krasavin E. A., Bonev M., Rettberg P., 1997, A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells, Appl. Environ. Microbiol., 63, 4377-4384.
• [39] Rees J.-P., De Wergifosse B., Noiset O., Dubuisson M., Jansens B., Thompson E. M., 1998, The origins of marine bioluminescence: turning oxygen defense mechanisms into deep-sea communication tools, J. Exp. Biol., 201, 1211-1221.
• [40] Ruby E. G., 1996, Lessons from a cooperative, bacterial-animal association: the Vibrio fischeri-Euprymna scolopes light organ symbiosis, Ann. Rev. Microbiol., 50, 591-624.
• [41] Ruby E. G., Morin J. G., 1979, Luminous enteric bacteria of marine fishes: a study of their distribution, densities and dispersion, Appl. Environ. Microbiol., 38, 406-411.
• [42] Shadel G. S., Devine J. H., Baldwin T. O., 1990, Control of the lux regulon of Vibrio fischeri, J. Biolumin. Chemilumin., 5, 99-106.
• [43] Sikora-Borgula A., Słomińska M., Trzonkowski P., Zielke R., Myśliwski A., Węgrzyn G., Czyż A., 2002, A role for the common GTP-binding protein in coupling of chromosome replication to cell growth and cell division, Biochem. Biophys. Res. Commun., 292, 333-338.
• [44] Słomińska M., Konopa G., Węgrzyn G., Czyż A., 2002, Impaired chromosome partitioning and synchronization of DNA replication initiation in an insertional mutant in the Vibrio harveyi cgtA gene coding for a common GTP-binding protein, Biochem. J., 362, 579-584.
• [45] Sun T. S., Stahr H. M., 1993, Evaluation and application of a bioluminescent bacterial genotoxicity test, J. AOAC Int., 76, 893-898.
• [46] Swift S., Throup J., Bycroft B., Willimas P., Stewart G., 1998, Quorum sensing: bacterial cell-cell signaling from bioluminescence to pathogenicity, [in:] Molecular microbiology, S. J. W. Busby, C. M. Thomas & N. L. Brown (eds.), Springer-Verlag, Berlin-Heidelberg, 185-207.
• [47] Thomulka K. W., Lange J. H., 1995, Use of the bioluminescent bacterium Vibrio harveyi to detect biohazardous chemicals in soil and water extractions with and without acid, Ecotoxicol. Environ. Safety, 32, 201-204.
• [48] Thomulka K. W., Lange J. H., 1996, A mixture toxicity study employing combinations of tributyltin chloride, dibutyltin dichloride, and tin chloride using the marine bacterium Vibrio harveyi as the test organism, Ecotoxicol. Environ. Safety, 34, 76-84.
• [49] Ulitzur S., 1989, The regulatory control of the bacterial luminescence system – a new view, J. Biolumin. Chemilumin., 4, 317-325.
• [50] Ulitzur S., 1998a, H-NS controls the transcription of three promoters of Vibrio fischeri lux cloned in Escherichia coli, J. Biolumin. Chemilumin., 13, 185-188.
• [51] Ulitzur S., 1998b, LuxR controls the expression of Vibrio fischeri luxCDABE clone in Escherichia coli in the absence of luxI gene, J. Biolumin. Chemilumin., 13, 365-369.
• [52] Ulitzur S., Barak M., 1988, Detection of genotoxicity and metallic compounds by the bacterial bioluminescence test, J. Biolumin. Chemilumin., 2, 95-99.
• [53] Ulitzur S., Martin A., Fraley C., Meighen E. A., 1997, H-NS protein represses transcription of the lux systems of Vibrio fischeri and other luminous bacteria cloned into Escherichia coli, Curr. Microbiol., 35, 336-342.
• [54] Ulitzur S., Weiser I., Yannani S., 1980, A new, sensitive and simple bioluminescence test for mutagenic compounds, Mutat. Res., 74, 113-24.
• [55] Van der Lelie D., Regniers L., Borremans B., Provoost A., Verschaeve L., 1997, The VITOTOX test and SOS bioluminescence Salmonella typhimurium test to measure genotoxicity kinetics, Mutat. Res., 389, 279-290.
• [56] Verschaeve L., Van Gompel J., Thilemans L., Regniers L., Vanparys P., Van der Lelie D., 1999, VITOTOX bacterial genotoxicity and toxicity test for the rapid screening of chemicals, Environ. Mol. Mutagen., 33, 240-248.
• [57] Winans S. C., Bassler B. L., 2002, Mob psychology, J. Bacteriol., 184, 873-883.
• [58] Ziegler M. M., Baldwin T. O., 1981, Biochemistry of bacterial bioluminescence, Curr. Topics. Bioeneng., 12, 65-113.