Simultaneous separation of chlorophylls and carotenoids by RP-HPLC in some algae and cyanobacteria from the southern Baltic

• Autorzy: Jodłowska S. , Latała A.
• Języki publikacji: EN

Abstrakty

EN

RP-HPLC (reversed-phase high-performance liquid chromatography) was used to analyse chlorophyll and carotenoid pigments in cyanobacteria and algae from the Baltic Sea, belonging to different taxonomic groups. The following species were used: Cyclotella meneghiniana - diatom, Oocystis submarina - green alga and Phormidium amphibium - cyanobacterium. Investigations on a favourable method of chlorophyll and carotenoid pigment separation have been carried out. This method allowed to separate the following pigments: lutein and zeaxanthin; diadinoxanthin and fucoxanthin; chlorophyll c1 and c2, into sharp peaks. It could be presumed that the method would be useful and universal in the identification of pigments in other algal and cyanobacteria species and natural phytoplankton assemblages.

Słowa kluczowe

EN

carotenoids; Xantophyll; chlorophyll; HPLC method; Cyclotella meneghiniana; oocystis submarina; Phormidium amphibium

• Wydawca: Institute of Oceanography University of Gdańsk
• Czasopismo: Oceanological and Hydrobiological Studies
• Rocznik: 2003
• Tom: Vol. 32, No. 2
• Strony: 81--89
• Opis fizyczny: Bibliogr. 17 poz., tab., wykr.

Twórcy

autor

Jodłowska S.

• Institute of Oceanography, University of Gdańsk, Al. Piłsudskiego 46 81-378 Gdynia, Poland

autor

Latała A.

• Institute of Oceanography, University of Gdańsk, Al. Piłsudskiego 46 81-378 Gdynia, Poland

Bibliografia

• [1]. Epler, K.S. & Sander, L.C. (1992), Evaluation of reversed-phase liquid chroma-tographic columns for recovery and selectivity of selected carotenoids. -J. Chromatogr. 595, 89-101.
• [2]. Garrido, J.L. & Zapata, M. (1997), Occurrence of phytylated chlorophyll c in Isochrysis galbana and Isochrysis sp. (clone T-ISO) (Prymnesiophyceae). - J. Phycol. 33, 209-214.
• [3]. Garrido, J.L. & Zapata, M. (1998), Detection of new pigments from Emiliania huxleyi (Prymnesiophyceae) by high-performance, liquid chromatography, liquid chromatography-mass spectrophotometry, visible spectros-copy, and fast atom bombardment mass spectrometry. - J. Phycol. 34, 70-78.
• [4]. Guillard, R.R.L. (1975), Culture of phytoplankton for feeding marine inverte-brates. In: Smith, W.L. & Chanle M.N. (eds): Culture of marine inverte-brate animals. - pp. 29-60, Plenum Press, New York.
• [5]. Jeffrey, S.W.; Mantoura, R.F.C. & Wright, S.W. (1997), Phytoplankton pig-ments in oceanography. - 661 pp.UNESCO Publishing, Paris.
• [6]. Kirk, J.T.O. (1994), Light and photosynthesis in aquatic ecosystems. - 509 pp., Cambridge University Press, Cambridge.
• [7]. Latała, A. & Misiewicz, S. (2002), Culture Collection of Baltic Algae (CCBA) at the Institute of Oceanography, University of Gdańsk. - Nova Hedwigia (in press).
• [8]. Mantoura, R.F.C. & Llewellyn, C.A. (1983), The rapid determination of algal chlorophyll and carotenoid pigments and t/wir breakdown products in natural waters by reverse-phase high-performance liquid chromatography. - Anal. Chem. Acta 151, 297-314.
• [9]. Rowan, K.S. (1989), Photosynthetic Pigments of Algae. - pp. 334. Cambridge Univ. Press, Cambridge.
• [10]. Roy, S. (1987), High-performance liquid chromatographic analysis of chloropigments. - J. Chromatogr. 391, 19-34.
• [11]. Sander, L.C. & Wise, S.A. (1993), Shape selectivity in reversed-phase liquid chromatography for the seperation of polar and non polar solutes. - J. Chromatogr. 656, 335-351.
• [12]. Sander, L.C.; Sharpless, K.E.; Craft, N.C. & Wise, S.A. (1994), Development of engineered stationary phase for the separation of carotenoid isomers. - Analyt. Chem. 66, 1667-1674.
• [13]. Schmid, H. & Stich, H.B. (1995), HPLC-analysis of algal pigments: compari-son of columns, column properties and eluents. - J. Appl. Phycol. 7, 487-494.
• [14]. Stanier, R.Y.; Kunisawa, R.; Mandel, M. & Cohen-Bazire, G. (1971), Purifica-tion and properties of unicellular blue-green algae (order Chroococ-cales). -Bac. Rev. June, 171-205.
• [15]. Strickland, I.D.H. & Parsons, T.R. (1972), A practical handbook of seawater analysis. -Bull. Fish. Res. Bd. Can. 167, 1-310.
• [16]. Van Heukelem, L.; Lewitus, A.J. & Kana, T.M. (1992), High-Performance Liquid Chromatography of phytoplankton pigments using a polymeric re-versed-phase C18 column. - J. Phycol. 28, 867-872.
• [17]. Wright, S.W.; Jeffrey, S.W.; Mantoura, R.F.C.; Llewellyn, C.A.; Bjornland, T.; Repeta, D. & Welschmeyer, N. (1991), Improved HPLC method for tlw analysis of chlorophylls and carotenoids from marine phytoplankton. -Mar. Ecol. Prog. Ser. 77, 183-196.