Molecular Analysis of Microorganisms Responsible for the First Phase of Nitrification in an Anoxic Environment

• Autorzy: Ziembińska A. , Ciesielski S. , Raszka A. , Miksch K.

Warianty tytułu

PL

Analiza molekularna mikroorganizmów odpowiedzialnych za prowadzenie pierwszej fazy nitryfikacji w środowisku anoksycznym

• Języki publikacji: EN

Abstrakty

EN

Ammonia-oxidizing bacteria communities were evaluated in a completely mixed, laboratory scale membrane reactor (MBR) working under anoxic conditions for 5 months. The microorganisms in activated sludge were fed a synthetic medium containing 66-150 mg NH4 +-N/l. The age of the activated sludge in MBR was 50 days and the hydraulic retention time (HRT) was 3.3 days. The estimation of the diversity and complexity of the AOB community together with the identification of the dominant bacteria in the activated sludge under anoxic conditions were performed using denaturing gradient gel electrophoresis (DGGE) and DNA sequencing. Molecular analysis of the microbial community carried out with two microbial molecular markers, 16S rRNA gene and amoA gene, suggested that nitrification was led by a Nitrosomonas-like species. In the biocenosis of the investigated bioreactor, oxygen was the crucial selective parameter. The results obtained in this work showed that amoA gene research is more suitable to study the stability and effectiveness of ammonia oxidation. This information emphasizes the necessity of the usage of molecular markers based on functional genes instead of ribosomal ones in order to present the actual state of the process performed in bioreactors. It was also stated that Nitrosomonas -like bacteria are able to perform nitritation even in anoxic environment, that is probably the reason why these bacteria are the most common AOB in different bioreactors.

PL

W eksperymencie badano grupę bakterii utleniających amoniak w bioreaktorze membranowym całkowitego wymieszania (MBR), pracującym w warunkach anoksycznych przez 5 miesięcy. Osad czynny zasilano pożywką syntetyczną, zawierającą 66-150 mg N-NH4 +/l. Wiek osadu wynosił 50 dni, a hydrauliczny czas zatrzymania - 3,3 dnia. Oszacowanie różnorodności i złożoności zbiorowiska bakterii utleniających amoniak oraz identyfikacja mikroorganizmów dominujących w badanym osadzie czynnym w warunkach anoksycznych została przeprowadzona metodą elektroforezy w gradiencie denaturacji (DGGE) i sekwencjonowania DNA. Analiza molekularna biocenozy, przeprowadzona z użyciem dwóch markerów molekularnych: genu kodującego 16S rRNA i genu kodującego monooksygenazę amonową (amoA), wykazała, że proces nitritacji był prowadzony przez gatunki bakterii z rodzaju Nitrosomonas. W biocenozie badanego bioreaktora stężenie tlenu było głównym parametrem selekcyjnym dla nitritatorów. W badaniach wykazano, że markerem molekularnym sprawdzającym się lepiej w monitoringu efektywności procesu nitryfikacji pierwszej fazy jest gen amoA. Te dane podkreślają, że w badaniach efektywności prowadzonego procesu zachodzącego w bioreaktorach, informacje uzyskiwane z markerów funkcjonalnych mają większą wartość niż uzyskane z markerów rybosomalnych. Stwierdzono również, że bakterie Nitrosomonas sp. są zdolne do prowadzenia nitritacji w warunkach anoksycznych i prawdopodobnie z tego powodu są najczęściej spotykaną bakterią utleniającą amoniak.

Słowa kluczowe

EN

16S rRNA gene; amoA gene; beta-proteobacterial ammonia oxidizers; DGGE; nested-PCR; Nitrosomonas-like bacteria

PL

analiza molekularna; gen amoA; gen16S rRNA; bakteria utleniająca amoniak; Nitrosomonas

• Wydawca: Institute of Environmental Engineering, Polish Academy of Sciences
• Czasopismo: Archives of Environmental Protection
• Rocznik: 2011
• Tom: Vol. 37, no. 1
• Strony: 89--98
• Opis fizyczny: Bibliogr. 25 poz., tab., wykr.

Twórcy

autor

Ziembińska A.

autor

Ciesielski S.

autor

Raszka A.

autor

Miksch K.

• The Silesian University of Technology, Faculty of Power and Environmental Engineering, Environmental Biotechnology Department,

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