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Antioxidant enzyme activities and lipid peroxidation in Mytilus galloprovincialis from the French Mediterranean coast

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EN
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EN
In the present work we have studied some of the indicators of oxidative damage of the digestive gland tissue of two populations of mussels Mytilus galloprovincialis: native mussels cultured in an aquaculture farm and contaminated mussels collected from the polluted marine area of the French Mediterranean coast located nearby Tamaris and La Seyne-sur-mer - small towns in close proximity to Toulon. Activities of antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPx) were analyzed, as well as the amounts of glutathione (GSH) and malonyldialdehyde (MDA). Glutathione is the most abundant low molecular cellular antioxidant, while malonyldialdehyde is the main product of lipid peroxidation. Additionally, the activity of lactate dehydrogenase-LDH, a marker of cell membrane damage, was determined. We have found an elevated activity of CAT and GPx in mussels from the contaminated areas -161.76 and 45.36 U mg^-1 protein, respectively vs. 98.68 and 29.84 U mg^-1 protein in the control mussels. The contaminated mussels also showed an elevated content of MDA -9.32 nmol mg-1 protein) vs. 7.57 nmol mg-1 protein in the control glands. The concentration of heavy metals (Fe, Mn, Zn, Cu, Ni, Pb, Cd) in the digestive gland, except lead, was significantly higher in mussels from the polluted area. Contrarily, the level of GSH in contaminated mussels was about two-fold lower than that in the control mussels -2.85 μmol GSH mg^-1 protein vs. 5.81 μmol GSH mg^-1 protein. At the same time, no significant differences in LDH activity were observed between the two mussel populations.
Rocznik
Strony
33--43
Opis fizyczny
Bibliogr. 31 poz., mapki, wykr.
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autor
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autor
Bibliografia
  • 1.Ardizzone G.D., Belluscio A., Gravina M.F., Somaschini A., 1996, Colonization and Disappearance of Mytilus galloprovincialis Lam. On an Artificial Habitat in the Mediterranean Sea Estuarine, Coast. Shelf Sci., 43: 665-676
  • 2.Beers R.F., Sizer I.W., 1952, Spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase, J. Biol.Chem., 195: 133-140
  • 3.Borg D.C., Schaich K.M., 1984, Cytotoxicity from coupled redox cycling of autoxidizing xenobiotics and metals. Israel J. Chem. 24: 38-53
  • 4.Canesi L. and Viarengo A., 1997, Age-related differences in glutathione metabolism in mussel tissues (Mytilus edulis L.), Comp. Biochem. Physiol., 116B: 217-221
  • 5.Charmasson S., Barker E., Calmet D., Pruchon A.S., Thebault H., 1999 Long-term variations of man-made radionuclide concentrations in a bio-indicator Mytilus galloprovincialis from the French Mediterranean coast. Sci. Total Environ., 237: 93-103
  • 6.Christie N.T., Costa M., 1984, In vitro assessment of the toxicity of metal compounds. IV. Disposition of metals in cells: interactions with membranes, glutathione, metallothioneins and DNA, Biol. Trace Elem. Res., 6: 462-473
  • 7.Ellman G.L., Courtney K.D., Andres K.J., Featherstone R.M., 1961, A new and rapid colorimetric determination of acetylcholinesterase activity, Biochem. Pharmacol., 7: 88-94
  • 8.Gamble S.C., Goldfarb P.S., Porte C., Livingstone D.R., 1995, Glutathione peroxidase and other antioxidant enzyme function in marine invertebrates (Mytilus edulis, Pacten maximus, Carcinus meanas and Asterias rubens), Mar. Environ. Res., 39: 191-195
  • 9.Gomez-Mendikute A., Cajaraville M.P., 2003, Comparative effects of cadmium, copper, paraquat and benzo[a]pyrene on the actin cytoskeleton and production of reactive oxygen species (ROS) in mussel haemocytes. Toxicol. in Vitro 17: 539-546
  • 10.Halliwell B, Gutteridge J.M., 1986, Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Arch Biochem Biophys. 246:501-514
  • 11.Halliwell B., Gutteridge J.M.C., 1989, Free Radicals in Biology and Medicine, 2nd ed. Clarendon Press, Oxford, pp. 128-130
  • 12.Lionetto M.G., Caricato R., Giordano M.E., Pascariello M.F., Marinosci L., Schettino T., 2003, Integrated use of biomarkers (acetylocholinesterase and antioxidant enzymes activities) in Mytilus galloprovincialis and Mullus barbatus in an Italian coastal marine area, Mar. Pollut. Bull.,46: 324-330
  • 13.Livingstone D.R., Garcia Martinez P., Michel X., Narbonne J.F., O'Hara S., et al., 1990, Oxyradical production as a pollutant-mediated mechanism of toxicity in the common mussels, Mytilus edulis L., and other mollusks. Funct. Ecol. 4: 415-424
  • 14.Lowry O.H., Rosenbrough N.J., Farr A.L., Randal R.J., 1951, Protein measurement with Folin phenol reagent, J. Biol. Chem., 193: 265-275
  • 15.Meister A., 1989, On the biochemistry of glutathione [In:[ Higashi N., Sakamoto T., Meister S. Glutathione Centennial. Molecular Perspectives and Clinical Implications edited by Taniguchi,. Academic Press, San Diego, CA, pp.3-22
  • 16.Porte C., Sole M., Albaiges J., Livingstone, D.R., 1991, Responses of mixed-function oxygenase and antioxidase enzyme system of Mytilus sp. to organic pollution, Comp. Biochem. Physiol., 77C: 211-219
  • 17.Power A., Sheehan D., 1996, Seasonal variation in the antioxidant defence systems of gill and digestive gland of the blue mussels, Mytilus edulis, Comp. Biochem. Physiol., 114C: 99-103
  • 18.Rabestein D.L., Guevremont R., Evans C.A., 1985, Glutathione and its metal complexes. [In:] Siegel, H. (Ed.), Metal Ions in Biological Systems. Marcel Dekker, New York, pp. 104-141
  • 19.Regoli F., Principato G., 1995, Glutathione, glutathione-dependent and antioxidant enzymes in mussel, Mytilus galloprovincialis, exposed to metals under field and laboratory conditions: implications for the use of biochemical biomarkers. Aquat. Toxicol., 31: 143-164
  • 20.Sara G., Manganaro A., Cortese G., Pusceddu A., Mazzola A., 1998, The relationship between food availability and growth in Mytilus galloprovincialis in the open sea (southern Mediterranean), Aquaculture, 167: 1-15
  • 21.Santos F., Gilson Z., Rocha J., Weis S., Fachinetto J., Favero A., Nogueira C., 2005, Diphenyl diselenide reverses cadmium-induced oxidative damage on mice tissue. Chem. Biol. Interact. 151, 159-165
  • 22.Santovito G., Piccinni E., Cassino A., Irato P., Albergoni V., 2005, Antioxidant responses of the Mediterranean mussel, Mytilus galloprovincialis, to environmental variability of dissolved oxygen. Comp. Biochem. Physiol. 140: 321-329
  • 23.Sedlak J., Lindsay R., 1968, Estimation of total protein-bound and nonprotein sulfhydryl groups in tissue with Ellman's reagent, Anal. Biochem., 26: 192-205
  • 24.Sole M., Lobera G., Lima D., Reis-Henriques M.A., Santos M.M., 2008, Esterases activities and lipid peroxidation levels in mussel tissue of the shanny Lipophrys pholis along several sites from the Portuguese Coast. Mar. Pollut. Bull. 56: 999-1007
  • 25.Stocks R., Dormandy, T.L., 1971, The autooxidation of human red cell lipids induced by hydrogen peroxide. Br. J. Hem. 20: 95-111.
  • 26.Torres M.A., Testa, C.P., Gaspari, C., Masutti, M.B., Panitz, C.M., Curi-Pedrosa, R., Alves de Almeida, E., Di Mascio, P., Filho, W. 2002. Oxidative stress in the mussel Mytella guyanensis from polluted mangroves on Santa Catarina Island, Brazil. Mar. Pollut. Bull. 44: 923-932
  • 27.Viarengo A., Canesi L., Pertica M., Poli G., Moore M.N., Orunesu M., 1990, Heavy metal effects on lipid peroxidation in the tissues of Mytilus galloprovincialis, Comp. Biochem. Physiol., 97C: 37-42
  • 28.Viarengo A., Nott J., 1993, Mechanisms of heavy metal cation homeostasis in marine invertebrates. Comp. Biochem. Physiol. C Comp. Pharmacol. Toxicol. 104: 355-372
  • 29.Wenning R.J., Di Giulio R.T., Gallagher E.P., 1988, Oxidant-mediated biochemical effects of paraquat in the ribbed mussel, Geukensia demissa, Aquat. Toxicol., 12: 157-170
  • 30.Winston G.W., Livingstone D.R., Lips F., 1990, Oxygen reduction metabolism by the digestive gland of the common mussel, Mytilus edulis L. J. Exp. Zool. 255: 296-308
  • 31.Wroblewski F., La Due J.S., 1955. Lactic dehydrogenase activity in blood. Proc. Soc. Exp. Biol. Med. 90: 210-213
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BUS8-0005-0044
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