Optimization research on biodegradation of hydrocarbon pollutions in weathering soil samples from manufactured gas plant (MGP)

• Autorzy: Steliga T.

Warianty tytułu

PL

Badania optymalizacyjne biodegradacji zanieczyszczeń węglowodorowych w zestarzałych glebach z gazowni klasycznej

• Języki publikacji: EN

Abstrakty

EN

The aim of this work is purification of contaminated soil from manufactured gas plants MGP, which arc not used. Prepared chromatographic methodologies, which allow qualitative identification and quantitative determination of individual aliphatic and aromatic (TAH, PAH) hydrocarbons in the soil from the selected MGP, arc presented. The results of the research on remediation of the soil polluted with petroleum hydrocarbons (TAH, PAHs) in semi-field conditions arc discussed. Application of basic biorcmediation and bioaugmcntation with indigenous microorganisms, enriched with PAH biodegradable fungi, resulted in reduction in amount of 5- and 6-polycyclic aromatic hydrocarbons. The research enables control of remediation progress, selection of optimal doses of biogenic compounds and determines the time of the process. The entire cycle of soil remediation was monitored with the use of gas chromatography (GC). Estimation of biodegradation degree of individual aliphatic hydrocarbons (alkancs) was based on changes in their concentration in the soil and determined by biodegradation indicators: C_l7/pristanc and C_18/phylane ratios. Attention was paid particularly to biodegradation of polycyclic aromatic hydrocarbons (PAH), which were observed in substantial concentrations in the polluted soil. The elaborated chromatographic methodology of PAH determination in the soil enabled estimation of a biodegradation rate referring to individual compounds. Moreover, attempts to create a TAH biodegradation model with the use of C_30_17&alpfa:(H),21β(H)-hopane were undertaken.

PL

Artykuł podejmuje zagadnienia związane z problemem oczyszczania gruntu skażonego w wyniku działalności klasycznych gazowni, które obecnie zostały wyłączone z eksploatacji. Przedstawiono opracowane metodyki chromatograficzne umożliwiające jakościową identyfikację i ilościowe oznaczenie poszczególnych węglowodorów alifatycznych i aromatycznych oraz oznaczenie ich sumarycznej zawartości (TAH, WWAs) w gruncie z terenu wytypowanej do badań gazowni. Omówiono wyniki badań w skali półtechnicznej oczyszczania gruntu skażonego zanieczyszczeniami węglowodorowymi (TAH, WWA) z wykorzystaniem biorcmediacji podstawowej i bioaugmentacji poprzez inokulację mikroorganizmami autochtonicznymi wzbogaconymi o grzyby zdolne do biodegradacji WWA, co uwidacznia się w obniżeniu zawartości 5- i 6-picrścicniowych WWA. Prowadzone badania pozwalają prześledzić przebieg procesu oczyszczania gruntu, dobrać optymalne dawki substancji biogennych i określić ramy czasowe prowadzonego procesu. Cały cykl oczyszczania gruntu monitorowano za pomocą chromatografii gazowej (GC). Oceny stopnia biodegradacji poszczególnych węglowodorów alifatycznych (n-alkanów) dokonano na podstawie zmian zawartości oznaczonych chromatograficznie oraz za pomocą przyjętych wskaźników stopnia biodegradacji: C_17/Pr i C_18/F. Zastosowanie biomarkera C-30_17α(H),21 β(H)-ho-panu do normalizacji stężeń TAH i WWA w trakcie przebiegu poszczególnych etapów biodegradacji pozwoliło na opracowanie modelu matematycznego opisującego kinetykę biodegradacji TAH i WWA.

Słowa kluczowe

EN

bioremediation; bioaugmentation; contaminated soil; manufactured gas plant; polycyclic aromatic hydrocarbons; indigenous microorganisms; fungi

PL

bioremediacja; bioaugmentacja; zanieczyszczenia gleby; wielopierścieniowe węglowodory aromatyczne; grzyby; oczyszczanie gleby

• Wydawca: Institute of Environmental Engineering, Polish Academy of Sciences
• Czasopismo: Archives of Environmental Protection
• Rocznik: 2008
• Tom: Vol. 34, no. 1
• Strony: 51--70
• Opis fizyczny: bibliogr. 78 poz., tab., wykr.

Twórcy

autor

Steliga T.

• Oil and Gas Institute ul. Lubicz 25A, 31-503 Kraków, Poland

Bibliografia

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