Abundance and morphotype diversity of surface bacterioplankton along the Gdynia - brest transect

• Autorzy: Żmuda M. J.
• Języki publikacji: EN

Abstrakty

EN

Abundance, biomass and morphotype diversity of bacterioplankton were analyzed in surface samples collected on a transect from Gdynia (Poland) to Brest (France) between 5 and 17 October, 2002. The total bacterial number ranged from 1.52 x 105 š 7.0 x 103 ml-1 (SE) to 4.08 x 106 š 3.1 x 105 ml-1. Samples from the Baltic Sea showed both the highest number of bacteria and the largest variability. Total bacterial biomass ranged from 5.45 š 0.99 to 128.17 š 13.58 ěg C l-1. The dominant morphotype were rods (82.4% to 95.7%) especially those with average cell volume below 0.1 ěm3. Regional differences in the average cell volume ranged from 0.099 to 0.382 ěm3. Autotrophic bacteria comprised on average 13.5 % of total bacterioplankton biomass, but increased to 48% at the west side of the English Channel. The high bacterial abundances discussed here shed light on the importance of the ocean surface as a habitat for prokaryotes and as a reservoir of bacterial biomass.

Słowa kluczowe

EN

marine bacteria; horizontal distribution; image analysis; number; biomass

• Wydawca: Institute of Oceanography University of Gdańsk
• Czasopismo: Oceanological and Hydrobiological Studies
• Rocznik: 2005
• Tom: Vol. 34, No.4
• Strony: 3--17
• Opis fizyczny: Bibliogr. 38 poz., tab., wykr.

Twórcy

autor

Żmuda M. J.

• Department of Antarctic Biology, Polish Academy of Sciences, Ustrzycka 10, 02-141 Warsaw, Poland,

Bibliografia

• [1]. Ameryk A., Mudryk Z., Podgórska В., 1999, The abundance, biomass and production of bacterioplankton in the Pomeranian Bay, Oceanologia 41, 389-401.
• [2]. Azam F., Fenchel Т., Field J. G., Gray J. S., Meyer-Reil L. A., Thingstad F., 1983, The ecological role of water-column microbes in the sea, Mar. Ecol. Prog. Ser., 10, 257-263.
• [3]. Barquero S., Botas J. A., Bode A. 1998, Abundance and production of pelagic bacteria in the southern Bay of Biscay during the summer, Sci. Mar., 62, 83-90.
• [4]. Blackburn N., Hagström A., Wikner J., Cuadros-Hansson R., and Bjørnsen P. K., 1998, Rapid determination of bacterial abundance, biovolume, morphology, and growth by neural network-based image analysis, Appl. Environ. Microbiol., 64,.3246-3255.
• [5]. Bölter, M., 1998. Structure of bacterial communities in Arctic permafrost soils (Tajmyr Penisinsula, Siberia), [in:] Polish Polar Studies 25th International Polar Symposium, Warszawa, 1998, Głowacki, P. and Bednarek, J. (eds.), Institute of Geophysics of the Polish Academy of Sciences, Warszawa, 61-66.
• [6]. Cho, B. C. and Azam, F. 1990, Biogeochemical significance of bacterial biomass in the ocean’s euphotic zone, Mar. Ecol. Prog. Ser., 63, 253-259.
• [7]. Donachie, S. P., 1995, Ecophysiological description of marine bacteria from Admiralty Bay (Antarctica), and the digestive tracts of selected Euphausiidae. [in:] Microbiology of antarctic marine environments and krill intestine, its decomposition and digestive enzymes, Rakusa-Suszczewski S. and Donachie S. P. (eds.), Polish Academy of Sciences. Department of Antarctic Biology, Warsaw, 101-196.
• [8]. Donachie, S. P., Christian J. C. and Karl D. M. (2001) Nutrient regulation of bacterial production and ectoenzyme activities in the subtropical North Pacific Ocean. Deep-Sea Research II48:1719-1732.
• [9]. Ducklow, H. W. 2000, Bacterioplankton production and biomass in the oceans. [in:] Microbial Ecology of the Oceans, Kirchman D.L. (ed.), Wiley-Liss, New York, 85-120.
• [10]. Ducklow, H.W. and Shiah, F. K. 1993, Estuarine bacterial production, [in:] Aquatic Microbiology, An Ecological Approach, Ford, Т., (ed.), Blackwell, London, 261-284.
• [11]. Eilers, H., Pernthaler J., Peplies J., Glöckner F. O., Gerdts G. and Amann R., 2001, Isolation of novel pelagic bacteria from the German Bight and their seasonal contribution to surface picoplankton, Appl. Environ. Microbiol., 67,5134-5142.
• [12]. Fuchs, M. B., Zubkov M. V., Sahm K., Burkill P. H., Amann R., 2000, Changes in community composition during dilution cultures of marine bacterioplankton as assessed by flow cytometric and molecular biological techniques. Environ. Microb., 2, 191-201.
• [13]. Fukuda R., Ogawa H., Nagata T. and Koike I., 1998, Direct determination of carbon and nitrogen contents of natural bacterial assemblages in marine environments, Appl. Environ. Microbiol., 64, 3352-3358.
• [14]. del Giorgio P.A. and Cole J. J., 2000, Bacterial energetics and growth efficiency, [in:] Microbial Ecology of the Oceans, Kirchman D.L. (ed.), Wiley-Liss, New York, 289-325.
• [15]. Giovannoni S. and Rappé M., 2000. Evolution, diversity, and molecular ecology of marine prokaryotes, [in:] Microbial Ecology of the Oceans, Kirchman D. L. (ed.), Wiley-Liss, New-York, 47-84.
• [16]. Gławdel M., Mackiewicz Т., Witek Z., 1999, Composition and abundance of pikoplankton in the coastal zone of the Gulf of Gdańsk, Oceanol. Stud. 28, 17-30.
• [17]. Gurienovich Т., 1995, Osobiennosti razvitia bakterioplanktona i bakteriobentosa raznotipnych rybohoziastviennyh vodojemov (Characteristic and development of bacterioplankton and benthic bacterial communities in different types of fishpond) PhD thesis, The State Scientific Institute for Fisheries (GosNIORH), Sankt-Peterburg (in Russian)
• [18]. Hara S., Terauchi K., Koike I., 1991, Abundance of viruses in marine waters: Assessment by epifluorescence and transmission electron microscopy, Appl. Environ. Microbiol., 57, 2731-2734.
• [19]. Jochem, F.J., 2001. Morphology and DNA content of bacterioplankton in the northern Gulf of Mexico: analysis by epifluorescence microscopy and flow cytometry, Aquat. Microb. Ecol., 25, 179-194.
• [20]. Jürgens K., Pernthaler J., Schalla S., Amann, R., 1999, Morphological and compositional changes in a planktonic bacterial community in response to enhanced protozoan grazing, Appl. Environ. Microbiol., 65, 1241-1250. Kirchman D. L., 2000. Uptake and regeneration of inorganic nutrients by marine heterotrophic bacteria, [in:] Microbial Ecology of the Oceans, Kirchman D.L. (ed.), NewYork, Wiley-Liss. pp. 261-288.
• [21]. Loferer-Krößbacher M., Klima J., and Psenner R., 1998, Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis, Appl. Environ. Microbiol., 64, 688-694.
• [22]. Mock Т., Meiners M. K., Giesenhagen H. C., 1997, Bacteria in sea ice and underlying brackish water at 54°26'5"N (Baltic Sea, Kiel Bight), Mar. Ecol. Prog. Ser., 158, 23-40.
• [23]. Porter K. G. and Feig Y. S., 1980, The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr., 25, 943-948.
• [24]. Putland, J.N. and Rivkin R.B., 1999, Influence of storage mode and duration on the microscopic enumeration of Synechococcus from cold coastal environment, Aquat. Microb. Ecol., 17, 191-199.
• [25]. Robarts R. D., Zohary Т., Waiser M. J., Yacobi Y. Z., 1996, Bacterial abundance, biomass, and production in relation to phytoplankton biomass in the Levantine Basin of the southeastern Mediterranean Sea, Mar. Ecol. Prog. Ser., 137, 273-281.
• [26]. Shiah F. and Ducklow H. W., 1994, Temperature regulation of heterotrophic bacterioplankton abundance, production, and specific growth rate in Chesapeake Bay, Limnol. Oceanogr., 39, 1243-1258.
• [27]. Sherr B. F., Sherr E. B., del Giorgio P., 2001, Enumeration of total and highly active bacteria, Paul J. (ed.), [in:] Methods in Microbiology, Marine Microbiology, Academic Press., London, Vol. 30, 129-159.
• [28]. Sieburth J. McN, 1979, Exposed Microbial Habitats, [in:] Sea Microbes, Oxford University Press, New York, 14-38.
• [29]. Sieracki M. E., Johnson W., Sieburth J., 1985, Detection, enumeration, and sizing of planktonic bacteria by image-analyzed epifluorescence microscopy, Appl. Environ. Microbiol., 49, 799-810.
• [30]. Simon M. and Azam F., 1989, Protein content and protein synthesis rates of planktonic marine bacteria, Mar. Ecol. Prog. Ser., 51, 201-213.
• [31]. Świątecki A., 1997, Zastosowanie wskaźników bakteriologicznych w ocenie jakości wód powierzchniowych (Aplication of bacteriological indexes in estimation of surface water quality), Studia i Materiały WSP Nr 118, Olsztyn (In Polish)
• [32]. Tuomi , P., Fagerbakke, K. M., Bratbak, G., Heldal, M. 1995, Nutritional enrichment of a microbial community: the effects on activity, elementary composition, community structure, and virus production, FEMS Microbiol. Ecol., 16, 123-134.
• [33]. Vosjan J. H. and Zdanowski M. K., 2001, Inter specific variability in UV-B sensitivity of Antarctic bacteria, CPJ, 16, 1-15.
• [34]. Whitman W. B., Coleman D. C. and Wiebe W. J., 1998, Prokaryotes: The unseen majority, Proc. Natl. Acad. Sci. USA, 95, 6578-6583.
• [35]. Zdanowski M. K. and Donachie S. P. 1993, Bacteria in the sea-ice zone between Elephant Island and South Orkneys during the Polish Sea-ice Zone Expedition, December 1988 to January 1989, Polar Biol., 13, 245-254.
• [36]. Zdanowski M. K. and Figueiras F.G., 1997, Relationships between bacterial abundance, other biota, and hydrographic variability in Ria de Vigo, Spain, Mar. Ecol. Prog. Ser„ 147, 257-267.
• [37]. Zimmermann R., 1977, Estimation of bacterial number and biomass by epifluorescence microscopy and scanning electron microscopy, [in:] Microbial ecology of a brackish water environment, Rheinheimer G. (ed.), Springer-Verlag, New York, 103-120.
• [38]. Zweifel U. L. and Hagström A., 1995, Total counts of marine bacteria include a large fraction of non-nucleoid-containing bacteria (ghosts), Appl. Environ. Microbiol., 61, 2180-2185.