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The expression of genes involved in the luminescence (lux genes) of many light-emitting bacteria, including the marine bacterium Vibrio harveyi, is regulated by a phenomenon called quorum sensing. The expression of the lux genes, and thus the efficiency of light emission, depends on the concentration of cells in the environment. Bacterial luminescence is effective when cells occur at high density, whereas light emission is negligible in diluted cultures. Quorum sensing regulation is a complex process which requires the functioning of many genes. The current paper describes the recently isolated novel V. harveyi mutant, which now appears to be impaired in quorum sensing. The mutant produces autoinducers normally, but it is partially defective in responding to these molecules, thus its quorum sensing reaction is delayed relative to wild-type bacteria.
Słowa kluczowe
Czasopismo
Rocznik
Tom
Strony
55--61
Opis fizyczny
Bibliogr. 19 poz., wykr.
Twórcy
autor
- Laboratory of Molecular Biology (affiliated with the University of Gdańsk), Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Kładki 24, 80-822 Gdańsk, Poland
autor
- Department of Molecular Biology, University of Gdańsk, Kładki 24, 80-822 Gdańsk, Poland
autor
- Department of Molecular Biology, University of Gdańsk, Kładki 24, 80-822 Gdańsk, Poland
Bibliografia
- [1]. Bassler B. L., Wright M., Silverman M. R., 1994, Sequence and function of luxO, a negative regulator of luminescence in Vibrio harveyi, Mol. Microbiol, 12, 403-412
- [2]. Belas R, Mileham A, Cohn D, Hilmen M, Simon M, Silverman M, 1982, Bacterial luminescence: isolation and expression of the luciferase genes from Vibrio harveyi, Science, 218, 791-793
- [3]. Chatterjee J, Miyamoto M, Meighen E.A, 1996, Autoregulation of luxR: the Vibrio harveyi lux-operon activator functions as a represor, Mol. Microbiol, 20, 415-425
- [4]. Czyż A, Zielke R, Konopa G, Węgrzyn G, 2001, A Vibrio harveyi insertional mutant in the cgtA (obg, yhbZ) gene, whose homologues are present in diverse organisms ranging from bacteria to humans and are essential genes in many bacterial species, Microbiology, 147, 183-191
- [5]. Dutkiewicz R, Słomińska M, Węgrzyn G, Czyż A. (2002) Overexpression of the cgtA (yhbZ, obgE) gene, coding for an essential GTP-binding protein, impairs the regulation of chromosomalfunctions in Escherichia coli. Curr. Microbiol, 45, 440-145
- [6]. Freeman J. A, Bassler B. L, 1999, A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi, Mol. Microbiol, 31, 665-677
- [7]. Freeman J. A. Lilley B. N., Bassler B. L., 2000, A genetic analysis of the functions of LuxN: a two-component hybrid sensor kinase that regulates quorum sensing in Vibrio harveyi, Mol. Microbiol., 35, 139-149
- [8]. Klein G., Żmijewski M., Krzewska J., Czeczatka M., Lipińska, В., 1998, Cloning and characterization of the dnaK heat shock operon of the marine bacterium Vibrio harveyi, Mol. Gen. Genet., 259, 179-189
- [9]. Kobayashi G., Moriya S., Wada C., 2001, Deficiency of essential GTP-binding protein ObgE in Escherichia coli inhibits chromosome partition, Mol. Microbiol., 41, 1037-1051
- [10]. Kok J., Trach K. A., Hoch J. A., 1994, Effects on Bacillus subtilis of a conditional lethal mutation in the essential GTP-binding protein Obg. J. Bacteriol., 176, 7155-7160
- [11]. Meighen E. A., 1994, Genetics of bacterial bioluminescence, Annu. Rev. Genet., 28, 117-139
- [12]. Miyamoto C. M., Chatterjee J., Swartzman E., Szittner R., Meighen E. A., 1996, The role of lux autoinducer in regulating luminescence in Vibrio harveyi; control of luxR expression, Mol. Microbiol., 19, 767-775
- [13]. Scott J. M., Haldenwang W. G., 1999, Obg, an essential GTP binding protein of Bacillus subtilis, is necessary for stress activation of transcription factor cP. J. Bacteriol., 181, 4653-4660
- [14]. Scott J. M., Ju J., Mitchell Т., Haldenwang W. G., 2000, The Bacillus subtilis GTP binding protein Obg and regulators of the cr® stress response transcription factor cofractionate with ribosomes. J. Bacteriol., 182, 2771-2777
- [15]. Swift S., Throup J., Bycroft B., Willimas P., Stewart G., 1998, Quorum sensing: bacterial cell-cell signaling from bioluminescence to pathogenicity, [in:] Molecular Microbiology, Busby S. J. W., Thomas C. M., Brown N. L. (eds.), Springer-Verlag, Berlin-Heidelberg, 185-207
- [16]. Ulanowska K., Sikora A., Węgrzyn G., Czyż A., 2003, Role of the cgtA gene function in DNA replication of extrachromosomal elements in Escherichia coli. Plasmid, 50, 45-52
- [17]. Vidwans S. J., Ireton K., Grossman A. D., 1995, Possible role for the essential GTP-binding protein Obg in regulating the initiation of sporulation in Bacillus subtilis. J. Bacteriol, 177, 3308-3311.
- [18]. Winans S. C., Bassler B. L., 2002, Mob psychology, J. Bacteriol., 184, 873-883
- [19]. Zielke R., Sikora A., Dutkiewicz R., Węgrzyn G., Czyż A., 2003, Involvement of the cgtA gene function in stimulation of DNA repair in Escherichia coli and Vibrio harveyi, Microbiology, 149, 1763-1770
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BUS5-0012-0028