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Chlorophyll fluorimetry as a method for studying light absorption by photosynthetic pigments in marine algae

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Using laboratory cultures of algae and natural phytoplankton populations from Nhatrang Bay (South China Sea), the relationship between the chlorophyll fluorescence F0, the chlorophyll a concentration Ca and light absorption capacities of algae cells was studied. It is shown that the ratio F0/Ca depends mainly on the species composition of the algae population; hence, the concentration Ca can be measured with the fluorescence method with acceptable accuracy only when the species composition of algae populations varies over a rather narrow range. The fluorescence F0 can, however, be a good index of the total absorption capacities of different phytoplankton species, because the intensity of F0 depends on the sum total of light absorbed by all photosynthetic pigments in a plant cell. Thus, the fluorescence F0 measures not only the concentration of chlorophyll a, but that of all photosynthetic pigment concentrations.
Czasopismo
Rocznik
Strony
519--531
Opis fizyczny
Bibliogr. 36 poz., tab., wykr.
Twórcy
  • Department of Biophysics, Faculty of Biology, Moscow State University, Moscow, 119899 Russia
autor
  • Department of Biophysics, Faculty of Biology, Moscow State University, Moscow, 119899 Russia
autor
  • Institute of Oceanology, Polish Academy of Sciences, Powstańców Warszawy 55, PL-81-712 Sopot, Poland
autor
  • Department of Biophysics, Faculty of Biology, Moscow State University, Moscow, 119899 Russia
autor
  • Pomeranian Pedagogical University, Arciszewskiego 22b, PL-76-200 Słupsk, Poland
  • Pomeranian Pedagogical University, Arciszewskiego 22b, PL-76-200 Słupsk, Poland
Bibliografia
  • [1] Antal T. K., Venediktov P. S., Konev Yu. N., Matorin D. N., Hapter R., Rubin A. B., 1999, Assessment of vertical profiles of phytoplankton photosynthetic activity by the fluorescence method, Engl. Okeanologiya, 39 (2), 287-292.
  • [2] Antal T. K., Venediktov P. S., Matorin D. N., Ostrowska M., Woźniak B., Rubin A. B., 2001, Measurement of phytoplankton photosynthesis rate using a pump-and-probe fluorometer, Oceanologia 43 (3), 291-313.
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  • [25] Ostrowska M., Matorin D. N., Ficek D., 2000b, Variability of the specific fluorescence of chlorophyll in the ocean. Part 2. Fluorometric method of chlorophyll a determination, Oceanologia, 42 (2), 221-229.
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  • [29] Tamiya H. K., Morimura K., Yokota M., Kunieda R., 1961, The mode of nuclear division in synchronous culture of Chlorella: comparison of various methods of synchronization, Plant Cell Physiol., 2, 383-403.
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  • [32] Woźniak B., Dera J., Ficek D., Majchrowski R., Kaczmarek S., Ostrowska M., Koblentz-Mishke O. I., 1999, Modelling the influence of acclimation on the absorption properties of marine phytoplankton, Oceanologia, 41 (2), 187-210.
  • [33] Woźniak B., Dera J., Ficek D., Majchrowski R., Kaczmarek S., Ostrowska M., Koblentz-Mishke O. I., 2000, Model of the ‘in vivo’ spectral absorption of algal pigments. Part 1. Mathematical apparatus, Oceanologia, 42 (2), 177-190.
  • [34] Woźniak B. , Dera J., Ficek D., Majchrowski R., Ostrowska M., Kaczmarek S., 2003, Modelling light and photosynthesis in the marine environment, Oceanologia, 45 (2), 171-245.
  • [35] Woźniak B., Dera J., Ficek D., Ostrowska M., Majchrowski R., Kaczmarek S., Kuzio M., 2002, The current bio-optical study of marine phytoplankton, Opt. Appl., 32 (1), 731-747.
  • [36] Yentsch C. S., Menzel D. W., 1963, A method for the determination of phytoplankton, chlorophyll, and phaeophytin by fluorescence, Deep Sea Res., 10, 221-231.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BUS5-0011-0077
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